Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"Retroviral vector"”.
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Insertional mutagenesis in zebrafish using a pseudotyped retroviral vector
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1997.
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Characterization of Genetically Modified Mesenchymal Stem Cells with a Retroviral Vector Expressing a Suicide Gene
… Here, we assessed whether transduction with a retroviral vector encoding cytosine deaminase gene might alter the stem cell property of mesenchymal stem cells. Mesenchymal stem cells were transduced at passage 1 and cultivated up to passage 11. We found that proliferative and differentiation …
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The Role of T Cell Receptor: Peptide MHC Affinity in T Cell Activation
… TCRs on the surface of T cells using a retroviral vector.
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Development of a gene therapy approach for the treatment of human mammary carcinoma using tissue specific retroviral vectors
… treatment for metastatic breast cancer. Retroviral vectors are attractive candidates for such in vivo gene therapy applications. Most of these vectors contain viral promoters that are not tissue specific. In order to specifically target malignant cells while at the same time sparing …
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Cellular Signaling in Development of the Cochlear Tonotopic Gradient
… the expression of DNER and PTP, as well as an retroviral vector to overexpress DNER, we observed disturbances of hair-bundle morphology and orientation. Our results suggest a role for DNER and PTP in hair-bundle formation and potentially specification of tonotopy.</p>
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Making In Utero Gene Therapy Safer and More Efficient: A First Step Towards Clinical Realization
… to improve the efficacy and safety of viral vectors. A two-vector delivery system was constructed to perform cell-specific in utero gene therapy, and I showed proof-of-principle that the system functions in vitro in human and sheep cells. An Ad5 serotype adenoviral vector (Ad5) acts as the …
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The Role of Pyruvate Dehydrogenase in Cell Growth
… encoding the PDHE1α protein were cloned into a retroviral vector which allowed doxycycline-inducible control of expression. Metabolism of cancer cells was studied in vitro using a combination of metabolomics and metabolic flux analysis. Growth in monolayer culture was performed in medium …
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The effect of nfa1 accination against Naeglearia fowleri infection
… pathogenesis of N. fowleri. In this study, the retroviral vector (pQCXIN) and the lentiviral vector (pCDH) cloned with the egfp-nfa1 gene was constructed and characterized for nfa1 DNA vaccination in mice. The expression of nfa1 gene in Chinese hamster ovary cell and human primary nasal …
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Structural determinants of murine leukemia virus (MLV) reverse transcriptase (RT) important for fidelity and drug resistance in vivo
Error-prone DNA synthesis by retroviral reverse transcriptases (RTs) is a major contributor to variation in retroviral populations generating mutants that exhibit altered host tropisms, resistance to antiviral drugs, and the ability to escape the host's immune system. To identify structural …
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Retroviral-mediated gene transfer of Il-1β?cDNA into human activated T cells
… (IL-Iβ) cDNA inserted into the MFG retroviral vector served as an indicator gene in these studies. Optimized methodologies resulted in T cell transduction frequencies of approximately 50-75%. An important consideration for the success of T lymphocyte-based gene therapy is the …
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Defining the biological role of FOXP3 in human CD4+ T cells
… constitutive expression mediated by a lentiviral vector, but not fluctuating expression driven by a retroviral vector, was sufficient to generate suppressive cells. Over-expression strategies were also used to characterize a novel splice isoform unique to human cells, FOXP3Δ2 (FOXP3b). 3) To …
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Manipulation of the Moloney Murine Leukemia Virus Envelope Protein in an Effort to Develop Directly and Indirectly Targeted Retroviral Vectors for Use in Human Gene Therapy
… lends itself to the use of replication deficient retroviral gene therapy vectors with interchangeable receptor binding sites. These vectors may be used separately or in combination with each other to ensure maximum delivery of a suicide gene only to cancerous cells involved with the primary tumor …
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Persistant GATA2 Expression Promotes Self-Renewal of Myeloid Progenitors and Blocks Lymphoid Differentiation.
… enforced expression of GATA2 using a MSCV GATA2 retroviral vector blocked differentiation of HSC and progenitors without inducing leukemia. We hypothesized that a lower dose of GATA2 can relieve the HSC block and eventually transform myeloid progenitors into leukemia stem cells. To test this …
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Generation of Transgenic Mice to Evaluate Promoter Activity and Specificity of Two Human Endogenous Retrovirus Long Terminal Repeats = Untersuchungen zur Promotor-Aktivität und -Spezifität von zwei Long Terminal Repeats humaner endogener Retroviren in transgenen Mäusen
… expression of therapeutic genes, delivered by retroviral vector systems, in a safe and efficient manner. The HERV-H-H6 LTR and the HERV-L LTR were chosen for the generation of transgenic mice. Their promoter activity and specificity had prior been tested in a luciferase expression vector in …
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Ex vivo Gentherapie der murinen Mukopolysaccharidose Typ VI durch retroviralen Transfer der humanen Arylsulfatase B cDNA
… des MPS VI Mausmodells durch einen ex vivo retroviralen Transfer der humanen ASB cDNA eine Verbesserung der Krankheitssymptome erreicht. Knochenmark von ASB -/- 129 Sv/j Mäusen wurde durch den retroviralen Vektor MSCV- ASB auf DNA-Ebene korrigiert. Vier Wochen nach Injektion des mit ASB cDNA …
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IGPR-1 promotes colorectal cancer tumor cell survival and modifies the response of cancer cells to chemotherapeutics
… lines, HCT116 and HT29, were transduced via a retroviral system to express IGPR-1 or empty retroviral vector pQCXIP. The effect of overexpression of IGPR-1 in HCT116 and HT29 cells was measured by MTT assay in non-adherent 24-well plates. In addition, cells were viewed under a light microscope, …
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The Role of Multi-Drug Resistance Associated Protein 4 and P-glycoprotein in Resistance of Neuroblastoma to Topotecan and Irinotecan
… in NB1691. Long term, stable expression of retroviral vector mediated short hairpin RNA (shRNA) reduced MRP4 and Pgp expression. Isogenic cell lines with reduced expression of MRP4 and Pgp exhibited an increase in sensitivity to both topotecan and SN-38, the active moiety of the prodrug …
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Identification of ITGBL1: A Novel Regulator of Adipogenesis
… C3H 10T1/2 mesenchymal stem cell line using a retroviral vector (pMSCV-puro), and assessed whether the cells were then more or less likely to differentiate into mature adipocytes when treated with an adipogenic hormonal cocktail. The overexpression did not result in any clear, repeatable …
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Etablierung stabiler Transfektanten für funktionelle Analysen des MALT1-Gens in t(11;18)(q21;q21)-positiven MALT-Typ-Lymphomen
Marginalzonen-B-Zell-Lymphome vom MALT-Typ stellen die häufigste Form primär extranodaler Non-Hodgkin-Lymphome dar. Sie entstehen auf dem Boden einer chronischen infektiösen oder autoimmunen Entzündung in hierdurch sekundär erworbenem lymphatischen Gewebe. Eine charakteristische und die häufigste …
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Analysis of DOCK2 and SENP2 mutations on the immune system and CD8 T cell survival
… setting, mutant OT-I T cells transduced with a retroviral vector expressing wildtype SENP2 preferentially survived, further confirming that SENP2 deficiency was responsible. Competitive bone marrow transplantation also showed a cell autonomous requirement for normal splicing of Senp2 to maintain …
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