Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 69 for “"RNA expression"”.
-
Dicer deletion and short RNA expression analysis in mouse embryonic stem cells
RNA interference (RNAi) manages many aspects of eukaryotic gene expression through sequence-specific interactions with RNA. Short RNAs, 20-30 nucleotides in length, guide the various effector proteins of RNAi to silence fully or partially complementary targets. The sequencing of endogenously …
-
Enhanced evaluation of RNA expression in Esophageal Cancer tissues: Combining Microarray and RNA-Sequence approaches
… EAC, and ESCC. To do this, microarray and bulk RNA-sequencing (RNA-seq) datasets were chosen from many independent studies and evaluated utilising, platform-specific techniques. For the microarray component, Affymetrix-based datasets were harmonised through rigorous preprocessing and subjected …
-
CHARACTERIZATION OF INFLUENZA NUCLEOPROTEIN BODY DOMAIN AS ANTIVIRAL TARGET
<p>Influenza is a segmented negative strand RNA virus. Each RNA segment is encapsulated by viral nucleoprotein (NP) and bound by the viral RNA dependent RNA polymerase (RdRP) to form viral ribonucleoproteins (vRNPs) responsible for RNA synthesis. NP is a structural component of the vRNP but also …
-
Vergleich der Genexpression im entzündlichen Kolonepithel und im kolorektalen Karzinom im Hinblick auf das erhöhte Tumorrisiko bei chronisch entzündlichen Darmerkrankungen
… Time PCR und der Immunfluoreszenzfärbung die RNA- und die Proteinexpression bestimmter Gene untersucht. Das Probenmaterial ist normales Kolonepithel von Menschen ohne CED, Kolonepithel von Patienten mit einer CED, Kolonpolyp- und Kolontumorgewebe von Menschen ohne CED. Die Ergebnisse der …
-
Statistical methods for the integrative analysis of single-cell multi-omics data
… single-cell studies are focused on quantifying RNA expression, transcriptomic readouts provide only a single dimension of cellular heterogeneity. Recently, technological advances have enabled multiple biological layers to be probed in parallel one cell at a time, unveiling a powerful approach …
-
Understanding the dynamics of rhythmic gene expression in mammalian cells
… core-clock mechanism also drives the rhythmic expression of downstream genes called clock-controlled genes, which are thought to be important for driving rhythmic biochemical and physiological processes. Mathematical models predict that for a gene to be rhythmically expressed, synthesis, …
-
Statistical methods for modeling RNA-Seq short-read data
… using the next-generation sequencing technology, RNA-Seq. Two methods are developed which attempt to accurately calculate RNA expression, the first using a penalized regression approach to remove bias based on nucleotide composition, as well as a second which demonstrates the use of variation as …
-
Nachweis von EGFR und ErbB2 Genamplifikationen in humanen Tumormodellen mittels Fluoreszenz-in-situ-Hybridisierung auf Gewebe- und Zelllinien-Mikroarrays
… die durch Genamplifikation oder Überexpression der Genprodukte aktiviert werden können. In der vorliegenden Arbeit wurden ca. 150 Xenografts und 30 Zellinien der Freiburger Xenograft Sammlung mittels Fluoreszenz-in-situ-Hybridisierung (FISH) auf das Vorliegen von EGFR- und ErbB2 …
-
Exploring Risk Factors of Alzheimer’s Disease Using Mouse Models
… to study whether they can mimic protein/RNA expression discrepancies, molecular changes, and enriched pathways found in human AD cases. The mouse models especially 5xFAD and APPNL-G-F show proteomic signatures similar to human AD but lack human-specific AD progressions, such as …
-
Expression der Gene ß-Globin, y-Globin und Glycophorin A in myeloischen Kolonien bei Kindern mit juveniler myelomonozytärer Leukämie
… mit RT-PCR und Southern-Blot auf eine Genexpression von beta-Globin, gamma-Globin und Glycophorin A untersucht. Tatsächlich läßt sich in myeloischen Kolonien von Patienten mit JMML eine Expression der RNA dieser Gene nachweisen. Allerdings läßt sich eine solche Expression auch in …
-
Epigenetic silencing of BRCA1 and maspin in sporadic breast cancer
The RNA expression of the tumor suppressor genes BRCA1 and maspin are frequently decreased or lost in sporadic breast cancer. We hypothesized that inactivation of these genes was due to aberrant epigenetic silencing at the level of gene transcription. In this study we show that aberrant cytosine …
-
Genetic and gene expression analysis of nasopharyngeal carcinoma (NPC)
… chromosomal copy number changes and differential RNA expression profiles in Nasopharyngeal carcinoma (NPC). Gene expression profiles identified a large number of differentially regulated genes involved in diverse functional processes, while genetic analysis detected extensive genomic abnormalities …
-
The spatial and temporal regulation of the transcriptome during plant development
… We detected specific light induced gene expression for the root phototropism gene RPT2 in the apical hook, and also phenotypes in Arabidopsis rpt2 mutants demonstrating that the gene is necessary for normal photomorphogenesis in the seedling apex. Significantly, expression of the …
-
Tracing RNA biography: in situ transcriptome profiling by novel spatial omics technologies
… shaped by the spatiotemporal regulation of gene expression. This intricate pattern of gene expression is, in part, attained through the precise regulation of mRNA: its metabolism, transport, and translation within individual cells across spatial and temporal dimensions. Therefore, it is critical …
-
Escherichia Coli ST131 Collected From Companion Animals Can Cause Cystitis and Pyelonephritis in a Murine Model of Urinary Tract Infection
… Isolates were assessed for motility via RNA expression of two flagellar genes, soft agar and trans-well assays. Adhesion was assessed via mannose sensitive hemagglutination, and T24 bladder epithelia adhesion assay. Finally, the percentage of a population of each isolate with type 1 …
-
Predicting Response to Neoadjuvant Chemotherapy in Breast Cancer with Gene Expression and Computational Pathology
… patient’s response to treatment based on gene expression and computational pathology. Firstly, I attempted to develop a clinically practical approach for classifying breast tumours using RNA from routine formalin fixed paraffin embedded (FFPE) histopathological samples. Breast cancers can be …
-
Molecular Characterization of the Heat Shock-Regulatedgroesl Operon in Lactobacillus Zeae Strain 102S
… promoter for use in food-grade heterologous gene expression systems. A plasmid-free derivative of Lactobacillus zeae ATCC 393, for years the type strain Lactobacillus casei, was studied because it is well characterized. PCR amplification with degenerate primers designed to conserved regions and …
-
Intranuclear strain measured by iterative warping in cells under mechanical and osmotic stress
… bound organelle and regulation center for gene expression in the cell. Mechanical forces transfer to the nucleus directly and indirectly through specific cellular cytoskeletal structures and pathways. There is increasing evidence that the transferred forces to the nucleus orchestrate gene …
-
Host and pathogen genomics: the molecular repertoire of soybean and Sclerotinia interactions
… screenings were carried out to identify soybean mRNAs and small RNAs that change in expression during early infection stages with S. sclerotiorum or its primary virulence factor, oxalic acid. Microarray expression data was obtained from a transgenic soybean line expressing oxalate oxidase (OxO), …
-
Functional Characterization of Metallothioneins in Arabidopsis and Barley
… quadruple mutant was also accompanied by altered RNA expression of several genes involved in Cu homeostasis including <em>FSD1, CSD1, COPT2, YSL2,</em> and <em>HMA5</em>.</p> <p>Although MTs are important for mobilization of Cu out of senescing leaves, this does not make a major contribution to …
Page 1 of 4