Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Quantitative real-time RT-PCR"”.
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The Effect of Gonadotropin Releasing Hormone on Opsin Gene Expression and Spectral Sensitivity in Zebra Cichlid Fish (Metriaclima zebra).
… in spectral sensitivity of cichlids has been partially attributed to differences in opsin gene expression, with each species preferentially expressing a subset of seven possible genes. Hormones such as gonadotropin releasing hormone (GnRH) can mediate changes in gene expression and the presence …
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Screening for kinases regulating subcellular localization of clock proteins using human kinome library
… transfection 9 D. Fluorescence microscopy 11 E. Quantitative real-time RT-PCR 12 F. Cell lysis 2 G. Western blotting 13 H. Immunoprecipitation (IP) and λphosphatase treatment 14 I. Fly genetics 15 J. Locomotor activity assays 16 Ⅲ. 결과 17 A. 인간 인산화 효소 라이브러리 구축 17 B. 인간 인산화 효소 스크리닝 20 C. …
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Classification of Neuronal Nicotinic Acetylcholine Receptors in Rat CA1 Hippocampal Interneuron Subpopulations Defined by Calcium-Binding Protein mRNA Expression
<p>In this study, the single-cell relative quantitative mRNA expression of three Calcium-binding proteins (CaBPs; calbindin, calretinin, parvalbumin) and eight nicotinic acetylcholine receptor (nAChR) subunits (alpha2-alpha5, alpha7, beta2-beta4) from interneurons from the stratum radiatum or …
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Nidogen-1 and Nidogen-2 in healthy human cartilage and in late-stage osteoarthritis cartilage
… Knorpel und in den späten Stadien der Osteoarthritis. Methoden: Die Lokalisierung und Quantifizierung beider Nidogene im Knorpel, sowohl auf Protein als auch auf mRNA-Ebene, wurde mittels gesunden Spendern und osteoarthritischem Knorpel von Patienten durchgeführt. Dabei wurden sowohl die …
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Identification of Anti-Beta2 Glycoprotein I Auto-antibody Regulated Gene Targets in the Primary Antiphospholipid Syndrome Using Gene Microarray Analysis
… CX3CL1, FGF18, ID2, SOD2, Tenascin C) using quantitative real-time RT-PCR analysis. This study revealed a complex anti-2GPI-regulated gene expression profile in HUVEC in vitro. The novel chemokines and pro-inflammatory cytokines identified in this study may contribute to the vasculopathy …
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MxA-Genexpression in peripheren mononukleären Blutzellen: ein sensitiver Marker für die Aktivität von endogenem und exogenem Typ-I-Interferon
In dieser Arbeit wurde ein Testsystem zum quantitativen Nachweis von Typ-I-IFN entwickelt, um die Aktivierung des IFN-Systems bei Patienten mit viralen Hepatitiden zu untersuchen. Es wurde eine quantitative Real-Time-RT-PCR etabliert, um die Menge an MxA mRNA in peripheren mononukleären Blutzellen …
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Functional Analysis of AtVAMP714 gene in Arabidopsis
… of the seedling axis. Sequencing the insertion locus revealed that the activation tag was positioned between ATP-binding protein and vesicle associated membrane protein genes. Cloned mutant locus and expression studies indicated that the vesicle associated membrane protein gene is …
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Prenatal and postnatal nutritional influences on leptin sensitivity and susceptibility to diet-induced obesity in the rat
… were then characterised by custom microarray and quantitative real-time RT-PCR (QPCR) analysis. Adult female offspring exposed to prenatal undernutrition (UN offspring) were found to exhibit leptin resistance in adulthood, independent of postnatal DIO. This result demonstrates for the first time …
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IL-23 and IL-27 expression in dendritic cells and the effects of HIV-1
… the production of IL-23 and IL-27, cytokines important in the generation of cellular immune responses. Dendritic cells were isolated from the epidermal and dermal skin layers resulting from elective abdominoplasties by CD1a and CD1c microbeads. Circulating DCs were isolated from peripheral blood …
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Evaluation of therapeutic effect of IKK epsilon SiRNA on breast cancer cells and development of a peptide-based SiRNA delivery system
The objective of this dissertation is to develop siRNA-based cancer therapeutics. In chapters 1 and 2, we introduced the mechanism, challenge and promise of RNAi-based therapy, as well as various siRNA delivery systems. In Chapter 3, we examined the therapeutic potential of IKKε siRNA on human …
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The Role of Annexin A6 in Osteoporosis
… for effective osteoporosis treatment and to further clarify its molecular mechanism of action. At first, ethanol or methanol extracts of 68 edible Korean native plants were screened and Poncirus trifoliata (PT) was selected as a final candidate because of its high inhibitory activity on …
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Untersuchungen zum molekularen Wirkmechanismus der antiinflammatorischen Aktivität von Sesquiterpenlactonen
… (SLs) auf molekularer Ebene durchgeführt, um die Kenntnisse für die Wirksamkeit dieser Substanzklasse zu erweitern und dem Modell, Sesquiterpenlactone als Leitstrukturen für neue entzündungshemmende Arzneistoffe zu verwenden, näher zu kommen. <br>1. 5 Furanoheliangolide aus Viguiera …
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Identifying and characterizing genes and transcription factors involved in coloration of apple fruit
… increasing over the years. One of the most important determinants of fruit quality is skin color. Red coloration in apple fruit is attributed to anthocyanin accumulation. Anthocyanins are encoded by structural genes, in the anthocyanin biosynthetic pathway, which are highly regulated by …
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Characterization of alternative NADH dehydrogenases in the respiratory chain of Toxoplasma gondii as a novel drug targets
… Tachyzoiten von Toxoplasma gondii generiert ein Membranpotential, über dessen Beitrag zum Energiestoffwechsel des Bradyzoiten- oder Ruhestadiums des Parasiten bisher wenig bekannt ist.Die steady state mRNA-Konzentration von 11 nukleär-kodierten Enzymen der Atmungskette wurde durch …
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Immune function alterations in Asian elephants (Elephas maximus) infected with Mycobacterium species
Tuberculosis is an important health concern for Asian elephant (Elephas maximus) populations worldwide. Most infections are due to Mycobacterium tuberculosis, the cause of human tuberculosis, though mechanisms underlying elephant susceptibility are unknown. In humans and other studied species, …
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The effects of honey on the inflammatory response of cells with respect to wound healing
… T cell proliferation, as demonstrated by use of artificial honey (a syrup of sugars as in honey). The ability for honey to induce messenger RNA expression for key cytokines involved in wound healing was investigated. Conventional reverse transcriptase-PCR was used to detect the production of mRNA …