Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"Quantitative image analysis"”.
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Fluorescence lifetime-resolved imaging microscopy studies: quantitative image analysis, spectral-FLIM, and photosynthesis
Development of instrumentation and image analysis methods for the fluorescence lifetime-resolved imaging microscopy (FLIM) were carried out in this thesis. With the new instrument setup and imaging processing algorithms, valuable information has been provided for studying in vivo photosynthesis …
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A Computer-based, Quantitative Image Analysis Of The Geometry Of Human Cerebral Arterial Bifurcations
… of blood flow. We have undertaken a detailed analysis of the geometry of cerebral bifurcations using a computer-based, video analysis system and frozen section techniques. The luminal surfaces of twenty bifurcations from whole brains obtained at autopsy from three human subjects were digitized …
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Quantitative Image Analysis in the Study of Neutrophil and Neutrophil-Like HL-60 Chemotaxis
… is this maximal concentration determined? With quantitative analysis of immunofluorescent fixed-cell images and live-cell migration videos, I demonstrate the role of microtubules in insulating the front and back modules of chemotactic neutrophils, and the role of ERK in driving neutrophil …
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Quantitative image analysis of in vivo microstructure and growth factor effects in hydroxyapatite bone scaffolds
… specific surface area (SSA). These measures were quantitatively compared by analyzing micro-computed tomography data and used to formally define and assess healing. An accurate custom image segmentation program was developed that utilizes grayscale values, the periodicity of the scaffold, and 3D …
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Ultrahigh Field Magnetic Resonance Imaging – Technical Development and Translational Applications
… imaging (MRI) may be used to provide detailed images of the human body with excellent soft tissue contrast. Alongside its current widespread clinical applications for diagnosis and treatment, MRI allows researchers to measure structure and function of different tissue types in order to advance …
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Epigenetic Changes in Alzheimer’s and Huntington’sS Disease : A semi-quantitative analysis of histone changes
… foundation of this current study. Initially semi-quantitative image analysis methods were developed to quantify histone changes. These methods were then used to investigate histone changes in the inferior temporal gyrus and hippocampus of AD and the motor cortex, sensory cortex and cingulate gyrus …
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Mueller-matrix-based analysis for second-harmonic generation imaging
… for imaging of biological structures. Invoking quantitative image analysis techniques facilitates the assessment of tissues in varying states. This thesis presents a quantitative description of the relative structural difference in collagen-based tissues by second harmonic generation (SHG) …
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Single-Cell Image Analysis Enables High-Throughput Phenotypic Drug Screen and Elucidates Cell-Fate Decision Principles
… signals cells experience. Here we applied quantitative image analysis to enable a one-pass multi-class phenotypic drug screen. Our combined experimental and computational approach can functionally annotate large compound libraries across diverse drug classes in a single-pass screen with …
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Single-Cell Image Analysis Enables High-Throughput Phenotypic Drug Screen and Elucidates Cell-Fate Decision Principles
… signals cells experience. Here we applied quantitative image analysis to enable a one-pass multi-class phenotypic drug screen. Our combined experimental and computational approach can functionally annotate large compound libraries across diverse drug classes in a single-pass screen with …
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Influence of Fibroblasts on Metastatic Cancer Cell Drug Resistance in a 3D Microfluidic Cell Array
… and device coculture. Additionally, preliminary quantitative image analysis showed that the coculture condition had lower apoptosis indicated by caspase-3 activity after Doxorubicin introduction compared to the control condition. With these findings, we can gain a better understanding into the …
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The Roles of Focal Adhesion Assembly in Membrane Localization of Ion Channels and Action Potential Shape
… technique with single cell electrophysiology and quantitative image analysis, we characterized local and global membrane distributions of Kir2.1, IK1 amplitude, and FAs. We established that membrane-bound Kir2.1 localizes in proximity to FAs, giving a non-uniform distribution of IK1 in the cell …
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Methods for optical microscopy beyond conventional limits
… seeks to produce accurate and precise enlarged images of an object. It is highly desirable, particularly in live-cell biological microscopy, that the observation process does not perturb the structure, nor interfere with the dynamics, of the sample. These requirements are at odds with the fact …
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Vertical distributions of megafauna on inactive vent sulfide features correspond to their feeding modes
… on megafaunal distributions using highresolution imagery of two inactive sulfide features, Lucky's Mound and Sentry Spire, to determine how taxonomic composition and feeding traits vary with vertical position on the features. A total of 51 morphotypes, each categorized to feeding mode, was …
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Vertical distributions of megafauna on inactive vent sulfide features correspond to their feeding modes
… on megafaunal distributions using highresolution imagery of two inactive sulfide features, Lucky's Mound and Sentry Spire, to determine how taxonomic composition and feeding traits vary with vertical position on the features. A total of 51 morphotypes, each categorized to feeding mode, was …
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Flame synthesis of carbon nanotubes and metallic nanomaterials
… and number density data were extracted from TEM images using a quantitative image analysis technique. The mean particle size for a precursor concentration of 6100 ppm was observed to increase from around 2 to 4 nm between 20 and 75 mm HAB.
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Dynamics of gene expression and signal transduction in single cells
… In this work, we combined time-lapse microscopy, quantitative image analysis and fluorescent protein reporters, which allowed us to directly observe multiple cellular components over time in individual cells. In conjunction with mathematical models, we have investigated three dynamical systems, …
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Automatisches Segmentieren von Mikroarraybildern
… in scientific as well as industrial research. Analysis of microarray images is a bottleneck of array data analysis pipelines, as it is usually performed using interactive computer programs. Apart from practical concerns, automation of microarray gridding and feature segmentation is most …
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Electron microscopy characterisation of size-selected Pd clusters and industrial Pd catalysts
… with a deposition energy of 500 eV. The quantitative image analysis allows one to gain insight into each catalytic Pd particle and, in combination with two-dimensional diameter measurements, evaluate the three dimensional morphology of the particles. The use of the cluster source has also …
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Tracing clonal cell plasticity in the microenvironment of pancreatic ductal adenocarcinoma
… signal, which enables cell sorting, quantitative image analysis, and effective decoding by statistical analysis. Using subcutaneous mouse models of pancreatic cancer, this thesis demonstrates that isogenic epitope labelling can detect epithelial clonal expansion events co-localised …
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Synthetic ground truth of biological shapes — Simulating variable Nuclear Pore Complexes for Microscopy
… Single Molecule Localization Microscopy (SMLM) images NPCs at protein-level resolution, whereupon image analysis methods study NPC variability. However, the true picture of NPC variability is unknown and the biological function of this variability is poorly understood. In quantitative image …
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