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Showing 1 to 20 of 54 for “"Pulse-Chase"”.
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Turnover Rate of The Neuronal Connexin Cx36 In Hela Cells
… half-lives measured to be 1.5 to 3.5 hours in pulse-chase analyses of connexins (Cx26 and Cx43) in tissue culture cells and whole organs. We utilized HaloTag technology to study the turnover rate of Cx36 in transiently transfected HeLa cells. The HaloTag protein forms irreversible covalent …
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Die Funktionelle Rolle der Palmitilierung des 5-HT 1A Rezeptor
… abhängig von der Proteinsynthese ist . Die pulse-chase Experimente demonstrieren dass die Rezeptormodifikation Stabil ist. Die zwei konservierten Cysteine 417 und 420 im C-terminal des Rezeptor wurden als potentiale Palmitoylierungsstellen identifiziert. Dies wurde durch die ortspezifische …
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STUDIES ON A CALCIUM-DEPENDENT ACTIVATOR PROTEIN, STRUCTURALLY AND FUNCTIONALLY HOMOLOGOUS TO CALMODULIN, FROM THE WATER MOLD ACHLYA AMBISEXUALIS RAPER.
… of the Saprolegniaceae studied. Calcofluor pulse-chase experiments indicate that sporangiogenesis involves a transition from tip growth to overall growth of walls. Anti-calmodulin drugs such as trifluoperazine (TFP) inhibit Achlya CaM-dependent activation of bovine PDE, spore germination, …
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Studies on the regulation of aspartokinase II from Bacillus subtilis
… and glucose limitation (t$\sb{1/2}$ = 120 min). Pulse-chase experiments demonstrated that aspartokinase II was stable during exponential growth; the synthesis of the enzyme rapidly declined in response to nutrient exhaustion. The degradation of aspartokinase II was interrupted by inhibitors of …
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Notch activation and clone fate in intestinal homeostasis and inflammation
… Using double nucleotide labelling and pulse-chase lineage tracing experiments along with immunofluorescence and immunohistochemistry methods with a transgenic Notch active mutant mouse model (N1ICD<sup>ΔIEC</sup>), cell and clonal dynamics were determined in the normal and inflamed …
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The Nuclear Pore Complex: Novel Glycoprotein Constituents
… screen for antibodies against the pore complex. Pulse chase analysis of labelled tissue culture cells showed that p62 is synthesized as a soluble cytoplasmic precursor, which is incorporated into the nuclear fraction with an unusually long half time of about six hours. Additionally, p62 is …
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Migration of Nucleocapsids in Vesicular Stomatitis Virus-Infected Cells is Dependent on Microtubules and Actin Filaments
… by which nucleocapsids migrate during infection, pulse-chase analysis of protein translation, plaque assays, and live cell imaging were used in conjunction with the BTBDM. In the work presented here, we show a necessity of both microtubules and actin filaments on nucleocapsid migration during …
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Vps1's Role From the Early Endosome to the Golgi
… Golgi proteins. We recently observed in FM4-64 pulse-chase experiment with vps1 null cells that the levels of accumulated FM4-64-carrying transport vesicles increased significantly, suggesting the involvement of Vps1 on both the endocytic traffic to the vacuole, and the recycling traffic passing …
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Vps1's Implication on Late Endosome-to-Vacuole Traffic
… sites and endosomal compartments. In a FM4-64 pulse-chase experiment, I found that the extent of colocalization of FM4-64 with Vps1-GFP drastically increased after 20 min chase, indicating Vps1-carrying vesicles are among the population of late endosomal compartments. Consistently, I observed …
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ΣΥΓΚΡΙΤΙΚΗ ΜΕΛΕΤΗ ΤΗΣ ΧΟΡΙΟΓΕΝΕΣΗΣ ΣΕ ΕΝΤΟΜΑ ΟΙΚΟΝΟΜΙΚΗΣ ΣΗΜΑΣΙΑΣ
… A RADIOACTIVE AMINOACID, AUTORADIOGRAPHY WITH PULSE-CHASE EXPERIMENTS, ONE AND TWO DIMENTIONAL ELECTROPHORESIS,AUTOFLUOROGRAPHY, SILVER STAINING METHOD OF GELS, IMMUNOCALIZATION OF PEROXIDASE WITH WESTERN BLOTTING AND DOUBLE ANTIBODY STAINING, PEROXIDASE LOCALIZATION IN THE LIGHT AND ELECTRON …
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Studies on the Teichoic Acids of the Pneumococcus
… to the wall teichoic acid is ruled out by pulse-chase studies.</p>
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Targeted 177 Lu antisense radiotherapy of B-cell non-Hodgkin's lymphoma
… PNA peptide. A comparison of the efficacy of a pulse/chase injection versus a single injection of the compound is also discussed.
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Caractérisation de la dégradation du récepteur des hormones thyroïdiennes TRB[bêta]1 et effet de RanBPM sur ce processus
… l'effet de RanBPM sur ce processus. Nos analyses pulse chase confirment que TR[bêta]1 est dégradé par la voie Ub-Pr et mettent en évidence une dégradation atypique de TR[bêta]1. En effet, en absence de T[indice inférieur 3], la dégradation de TR[bêta]1 débute plus de 30 minutes après le début de …
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FSP1/S100A4-expressing stem/progenitor cells are essential for TMJ growth and homeostasis
… GLI1 ja Scleraxis, kanssa. BrdU Pulse Chase -kokeet osoittivat FSP1-syyruston osajoukon säilyttävän merkkiaineen, mikä viittaa FSP1:n leimaavan uuden kanta- ja esisolupopulaation kondyylissä. FSP1:tä ilmentävien solujen tuhoaminen ehdollisen difteriatoksiiniaktiivisuuden avulla …
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Mechanismus der Neuentstehung von Peroxisomen in Saccharomyces cerevisiae
… Um diese Frage zu klären, wurden Pulse-Chase-Experimente mit verschiedenen Hefestämmen mit steuerbaren Genen für Pex3-Markerproteine etabliert.Die Untersuchung mit dem Hefestamm, der in der Anwesenheit von Galactose CFP-Pex3p und in der Anwesenheit von Kupfer YFP-Pex3p induzierte, …
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Analyzing large-scale chromatin fiber via studying cytology of DNA replication and in vivo immunogold labeling
… chromatin fiber during replication. A novel pulse-chase condition was applied to track the structural change of newly replicated DNA. Minuscule sized nanobody was labeled with nanogold particle via an innovated labeling method, and then integrated into several in vivo loading techniques to …
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Estrogen Regulates Vitellogenin Gene Transcription and Messenger-Rna Degradation in Xenopus Laevis Liver
… were measured in Xenopus laevis liver using pulse labeling and pulse chase techniques. Estrogen regulates the accumulation of vitellogenin mRNA through three processes: (1) There is a selective transcriptional activation of the vitellogenin genes from an essentially off mode to a completely …
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Studies on Translation Speed and Protein Folding Efficiency in Bacteria and Eukaryotes
… or both types of interactions was developed. By pulse-chase analyses in living E. coli cells it was demonstrated that sequence engineering based on these concepts predictably modulate translation rates due to changes in polypeptide elongation and show that such alterations significantly impact …
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A Transmembrane Precursor of Secretory Component
… of human SC in a cell-free translations and pulse labelling of cells, SC is made as a single larger precursor with an approximately 15 kilodalton cytoplasmic domain. In pulse-chase experiments, the carbohydrate moieties of the precursor are first converted to the complex type and the …
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Antibiotics that Inhibit 30S or 50S Ribosomal Subunit Formation: Hygromycin B, Quinupristin-Dalfopristin and XRP 2868.
… and <em>Streptococcus pneumoniae</em>.</p><p>Pulse chase experiments were performed which verified slower rates of ribosomal subunit formation in drug treated cells. Hygromycin B exhibited a concentration dependent inhibitory effect on viable cell number, growth rate, protein synthesis and 30S …
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