Global ETD Search
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Showing 1 to 20 of 29 for “"Polyketide synthase"”.
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STRUCTURE DETERMINATION, BIOCHEMICAL CHARACTERIZATION, AND PROTEIN ENGINEERING OF THE ACYL CARRIER PROTEIN DOMAIN FROM 6-DEOXYERYTHRONOLIDE B SYNTHASE
Polyketides are an important class of natural products. 6-Deoxyerythronolide B Synthase (DEBS) is a type 1 polyketide synthase that produces the Polyketide antibiotic drug erythromycin. DEBS is a large multi-domain enzyme complex containing multiple enzymatic domains, including acyl carrier protein …
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Novel Mechanisms in Zebrafish Otolith Nucleation, Tethering, and Dysfunction
… in three zebrafish mutants with mutations in polyketide synthase, 2) otolin-1b is a putative component of the cuticular plate and involved with otolith growth and maintenance, and 3) otolin-1a, a component of the otolithic membrane, could be used to monitor risk for vestibular dysfunction …
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The design of a molecular assembly line based on biological molecules
… on the principles of the biological molecules polyketide synthase and kinesin, and in some embodiments, may employ biomolecules like DNA as components of the system. The scheme entails the construction of a polymeric chain that moves a shuttle along the chain by controlling the interactions …
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Protein and Pathway Engineering for Biosynthesis or Aromatic Compounds
A type I fatty acid synthase, FAS-B from Brevibacterium ammoniagenes, and a type I polyketide synthase, 6-MSAS from Penicillium patulum, were successfully engineered via rational pathway design to synthesize TAL in vivo with a maximal yield of 1.7 g/L. The direct biosynthesis of phloroglucinol from …
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Dual-omics approach for 3,4-dihydroisocoumarin characterisation from Hydrangea serrata and isolation of polyketide synthases for heterologous expression in Pseudomonas putida KT2440
… mechanism is well known from the chalcone synthase superfamily, a group of plant specific Type III polyketide synthases. Chalcone synthases and their homologues, the stilbene synthases produce a huge amount of specialised plant metabolites including phenylpropanoids. The dihydroisocoumarin …
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Untersuchungen auf Sekundärstoffproduktion und physiologische Charakterisierung von marinen heterotrophen Bakterien aus dem deutschen Wattenmeer
… was applied, in which bacteria were screened for polyketide synthase-, FADH2-halogenase- and non-ribosomal peptide synthetase genes. Additionally agar diffusion assays were applied to investigate the antagonistic activity of the strains, and some bacteria were tested for acylated homoserine …
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Investigating intermediates in 6-methylsalicylic acid biosynthesis
… acid (6-MSA) is one of the oldest known polyketides. It is synthesised in vivo by the polyketide synthase 6-methylsalicylic acid synthase (6-MSAS), a multifunctional enzyme which uses its active sites iteratively. The stereochemistry of the hydroxyl produced from the single ketoreduction, …
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THE ROLE OF PKS1 MEDIATED SECONDARY METABOLISM IN HOST PENETRATION AND COLONIZATION DURING RICE BLAST DISEASE
… ELUSIVE. IN THIS STUDY, WE DESCRIBE A PKS1 (POLYKETIDE SYNTHASE) CONTAINING GENE CLUSTER, WHICH IS ESSENTIAL FOR THE APPRESSORIAL FUNCTION. PKS1 DELETION MUTANT GENERATES NON-FUNCTIONAL APPRESSORIA THAT FAIL TO ELABORATE A PROPER PENETRATION PORE OR CONSEQUENTLY FAIL TO PENETRATE THE HOST …
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Biosynthetic characterisation of secondary metabolites from stromatolite cyanobacteria Symploca sp. 1013
… metabolites are formed due to the presence of polyketide synthase (PKS) or non-ribosomal peptide synthetise (NRPS). Bioactivities in areas such as antibacterial, antifungal and anticancer have been implicated in many of these metabolites. In the present study, various marine cyanobacterial …
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Fatty acid metabolism of marine microalgae
… of Schizochytrium which was shown to employ a polyketide synthase (PKS) complex for primary DHA biosynthesis. Additionally, genes of the Schizochytrium PUFA-PKS were successfully expressed in transgenic yeast, which produced DHA. Candidates for genes encoding C. cohnii PUFA-PKS components and …
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Investigation of Specialized Metabolites in Apicomplexan Parasite Life Cycles
… parasites, we have begun to characterize a polyketide synthase (PKS) with unknown resultant products. Together, this work provides the basis for understanding how specialized metabolites within the mosquito microbiome affect the Plasmodium parasite transmission capacity while also …
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Studies on the biosynthesis of oudenone and verucopeptin
… ( 89 ) is structurally characterized by a polyketide-derived tetrahydropyranyl side chain and a 19-membered cyclodepsipeptide. A DNA fragment of 72.5 kb was isolated from a cosmid library of Actinomadura verrucosospora chromosomal DNA. Partial analysis and sequencing of this gene cluster …
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Characterizing and Engineering Thioesterases as Biocatalysts for Macrocyclization
… Finally, the first extensive engineering of a polyketide synthase (PKS) TE was undertaken via a rational protein design approach and an LCMS-based screening method. Four variants were identified with improved macrocyclization activity over wild-type (WT), highlighting the utility and success of …
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A Search for the Counter-Immune Mechanisms of Mycobacterium Tuberculosis
… nitric oxide by inducible nitric oxide synthase (NOS2) and is essential for mammalian control of Mtb infection. Mice lacking NOS2 are unable to control replication of Mtb and rapidly succumb to infection. The persistent nature of TB infection suggests that Mtb has evolved counter-immune …
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A genomics-led approach to deciphering heterocyclic natural product biosynthesis
… A-D are a group of oxazole trienes produced by a polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) pathway in Streptomyces sp. KO-7888 and Streptomyces sp. OM-5714. The phthoxazolin pathway was used as a model to study 5-oxazole and primary amide formation in PKS-NRPS pathways. An …
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Identification and characterization of ice nucleation active bacteria isolated from precipitation
… for the activity may lie within a type-1 polyketide synthase/non-ribosomal peptide synthase gene cluster. This gene cluster is absent from the genomes of all non-ice+ strains of Lysinibacillus, and contains mutations in five of the nine ice nucleation inactive mutants generated from the …
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Estudio genómico, transcriptómico y proteómico del proceso de infección del hongo fitopatógeno botrytis cinerea
… BcPKS7 and BcPKS20, responsible for encoding polyketide synthase (PKS) enzymes in B. cinerea, were examined, with an initial focus on the production of botrycinereic acid. However, the generation of knock-out mutants for these genes did not reveal significant changes in growth, sporulation, …
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Genomics and transcriptomics of the industrial acarbose producer Actinoplanes sp. SE50/110
… several new non-ribosomal peptide synthetase-, polyketide synthase and hybrid-clusters were identified on the Actinoplanes genome. Another key finding represents the discovery of a functional actinomycete integrative and conjugative element, which might pose an elegant way of genetically …
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