Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 22 for “"Phospholamban"”.
-
Thermodynamic effects of phospholamban on Ca-ATPase kinetics
… In the heart, the Ca-ATPase is regulated by phospholamban, which inhibits the Ca-ATPase by decreasing Ca-ATPase calcium sensitivity. However, the kinetic and thermodynamic mechanisms of inhibition are not understood. The purpose of this research was to test the hypothesis that phospholamban …
-
Examining the Role of Phospholamban Phosphorylation on Interaction with SERCA Using Fluorescence Microscopy
… Reticulum Ca2+-ATPase (SERCA) by Phospholamban (PLB) plays a crucial role in normal cardiomyocyte function through controlling the speed and extent of myocyte relaxation. The interaction between PLB and SERCA is altered in many forms of heart failure (HF), making these proteins …
-
An Investigation of the Phospholamban-Serca Regulatory Interaction with Fluorescence Resonance Energy Transfer
<p>With Fluorescence Resonance Energy Transfer (FRET), we are able to detect changes in the structure and affinity of the PLB-SERCA regulatory complex in live cells. Using this approach, we have detected a high level of PLB-SERCA interaction even at Ca2+ concentrations known to fully relieve PLB …
-
Mutations in Phospholamban Alter the Structure and Function of the Calcium ATPase Regulatory Complex
<p>Phospholamban (PLB) is an integral membrane protein that plays an important role in regulation of cardiac calcium handling and contractility. PLB exists as a homopentamer in the membrane, which upon deoligomerization into active monomers reversibly inhibits sarco/endoplasmic reticulum calcium …
-
The Role of Phospholamban Cysteines in the Activation of the Cardiac Sarcoplasmic Reticulum Ca2+ Pump by Nitroxyl (HNO)
Phospholamban (PLN) is an integral membrane protein that regulates the sarco(endo)plasmic Ca2+-ATPase (SERCA2a) within the cardiac sarcoplasmic reticulum (CSR). SERCA2a regulates intracellular Ca2+- handling and thus plays a critical role in initiating cardiac contraction and relaxation. It is …
-
Identifizierung und Funktion von Effektorproteinen der NO/cGMP Signalkaskade
… isoliert. Dabei ergab sich die Assoziation von Phospholamban, einem Substrat der cGMP-Kinase, das den Calcium Rücktransport durch die Ca2+-ATPase in Speichervesikel des sarkoplasmatischen Retikulums moduliert. Die Interaktion von Phospholamban mit der cGMP-Kinase I β und dem IP3-Rezeptor Typ I …
-
The Function Of Sarcoplasmic Reticulum In The Aged Rat Heart
… phosphorylation of phospholamban in situ was significantly compromised with aging (38%), which was associated with an age-related reduction in the isoproterenol-stimulated accumulation of tissue cyclic AMP (18%). However, the cyclic AMP-dependent phosphorylation of …
-
Sex differences in the cardiac beta-adrenergic receptor contractile response
… or 100 nM ISO, while females displayed enhanced phospholamban phosphorylation only at 100 nM ISO. Females appear to show compensatory increases in adenylyl cyclase expression, cAMP accumulation and phospholamban phosphorylation. The mechanism(s) for the reduced &beta-adrenergic contractile …
-
Towards the full molecular investigation of protein kinase a mediated catalysis by NMR spectroscopy.
… reticulum Ca2+-ATPase (SERCA), and phospholamban (PLN). Phosphorylation of PLN by PKA is associated with an augmented rate of SR Ca2+ uptake and relaxation of the myocardium. Mutants of PLN (R9C-PLN and R14Del-PLN) have previously been shown to be linked with forms of the fatal …
-
Die Hemmung der PI3K verstärkt die Kontraktilität in alpha1-adrenerg stimuliertem Myokard
… dass in WM-inkubierten Kerzmuskelzellen PE die Phospholamban (PLN) Phosphorylierung und die intrazelluläre Ca2 + Konzentration im Vergleich mit den Kontrollezellen signifikant verstärkt. Zusammengefasst konnte diese Studie erstmalig zeigen, dass es durch Hemmung der PI3K, durch einen Anstieg der …
-
Properties And Regulation Of The Calcium Ion Pump In Heart And Skeletal Muscle Sarcoplasmic Reticulum
… inhibitor phospholamban. This thesis examines the Ca{dollar}sp{lcub}2+{rcub}{dollar} pump and its regulation in different tissues (cardiac, slow-twitch and fast-twitch muscle SR: CMSR, SMSR and FMSR) and different stages in development (fetal, newborn and …
-
Candidate Gene Expression and SNP Analyses of Toxin-Induced Dilated Cardiomyopathy in the Turkey(Meleagris gallopavo)
… candidate genes including troponin T (cTnT) and phospholamban (PLN) using quantitative reverse transcription polymerase chain reaction (RT-PCR) in toxin-induced DCM affected and unaffected turkeys. Cardiac TnT and PLN were chosen because their spontaneous expression has been reported to be …
-
Etablierung einer Zellkultur aus unterschiedlichen humanen Herzmuskelgeweben sowie die Simulation von verschiedenen Mangelzuständen in vitro
… Gene hANP, hBNP, GATA-4, Serca 2, Ryanodin 2, Phospholamban und NCX in der RT-PCR bestimmt. Im Western-Blot konnte zusätzlich Serca 2a nachgewiesen werden. <br>Bei den einzelnen Induktionsversuchen fiel auf, dass für ein optimales Wachstum der Zellen der Zusatz von Serum nötig war. Bei …
-
Effects of magnesium and calcium upon excitation-contraction coupling in the heart
… in the activity of an SR membrane protein, phospholamban (PLB), were investigated. Phospholamban phosphorylation increases the rate of Ca 2 uptake into the SR and it was observed that PLB phosphorylation in all 3 strains also increases with postnatal age. There seems then to be a coordinate …
-
Analysis of genetic variations associated with arrhythmogenic right ventricular cardiomyopathy
… performed candidate gene screening of the phospholamban (PLN) gene, genome-wide copy number variant (CNV) analysis and whole exome sequencing to identify the causal genetic variant. The ACM2 family harboured no disease-causing PLN variants. However, on screening all cardiomyopathy cases in …
-
Probing cardiac calcium regulation using time-resolved optical spectroscopy.
… heart, SERCA activity is partially inhibited by phospholamban, another integral membrane protein. This inhibition can be relieved by high Ca2+ concentration or phosphorylation of PLB. Misregulation of Ca2+ by the SERCA-PLB complex is associated with heart failure. The goal of this thesis is to …
-
Physical Mechanisms of Ca-ATPase Regulation in the Heart
… allosteric regulation by the phosphoprotein phospholamban (PLN). The Ca-ATPase is thought to be a target for nitrative stress and is affected by several chronic diseases of the heart. In the heart, age-based nitration of the Ca-ATPase inhibits Ca²⁺ transport activity but the physical …
-
Energy Landscapes of Hybrid Potentials Based on NMR Data
… of transmembrane proteins sarcolipin and phospholamban, achieved by introducing ssNMR orientational constraints in the hybrid potentials. Together, the structural and orientational constraints in the hybrid potential setup proved to be sufficient to represent the environment for membrane …
-
Veränderungen der Calcium-Homöostase als mögliche Ursache einer linksventrikulären Funktionsstörung im humanen "hibernating myocardium"
… die sarkoplasmatische Calcium-ATPase (SERCA2a), Phospholamban (PLN), Natrium-Calcium Austauscher (NCX) und Ryanodin-Rezeptor (RyR2) waren daher Gegenstand der Untersuchungen und sollten im Einzelnen quantifiziert werden. Zusätzlich wurde das Hitzeschockprotein hsp70 untersucht, welches eine …
-
The Novel Role of Hematopoietic Lyn Substrate-1 Associated Protein X-1 in Cardiac Contractility and Cardioprotection
… of sarcoplasmic reticulum (SR) calcium uptake, phospholamban (PLN) and sarco(endo)plasmic reticulum calcium ATPase (SERCA), suggesting its role in regulating cardiomyocyte calcium homeostasis. Moreover, HAX-1 level is reduced in cardiac ischemia/reperfusion injury, implicating the participation …
Page 1 of 2