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Showing 1 to 14 of 14 for “"Pepck"”.
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Conformational Entropy in PEPCK Catalysis: Dynamic Motions Critical to Function
… of interest, phosphoenolpyruvate carboxykinase (PEPCK), has multiple dynamic motions that work in concert during the catalytic cycle. One of these mobile elements, termed the omega-loop due to its architecture, acts like a lid that closes over the active site and has been hypothesized to serve …
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Proteins and instability elements involved in phosphoenolpyruvate carboxykinase mRNA turnover
… by mechanisms that affect the level of the PEPCK m RNA and subsequently determine the level of the PEPCK protein. In the liver, various factors including glucocorticoids, thyroid hormone, and glucagon regulate the transcription of the PCK-1 gene; while in the renal proximal tubule, it is …
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Non-Canonical Roles of Cytosolic Phosphoenolpyruvate Carboxykinase in Small Intestine Metabolism
Phosphoenolpyruvate carboxykinase (PEPCK) is considered by many to be the quintessential regulatory enzyme of gluconeogenesis. This common perspective of PEPCK solely functioning in gluconeogenesis is too narrow and does not do justice to the complexity and number of pathways in which this enzyme …
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The effect of magnesium deficiency on the gluconeogenic enzymes with emphasis on the effects of fasting and anorexia
… (FDPase), and phosphoenolpyruvate carboxykinase (PEPCK). In Experiments II and III rats were meal-fed diets adequate or deficient in magnesium; in addition, a group of rats was pair-fed to the magnesium-deficient group to test for the effects of anorexia. After 17 days the rats were fasted for 20 …
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Structure-Function Studies on Two Phosphoenolpyruvate Carboxylases
Phosphoenolpyruvate carboxykinase (PEPCK) and phosphoenolpyruvate carboxylase (Pepc) are two important CO₂-fixation enzymes which share a similar reaction mechanism. Both operate through a lid-gated active site and have a hypothesized enol-pyruvate intermediate in their catalytic pathway. While …
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Development and validation of a cell based model of insulin resistance and investigation into the intracellular molecular defects induced by diabetes and obesity
… gene, phosphoenolpyruvatecarboxykinase (PEPCK). Cells cultured in serum from obese diabetic human subjects exhibited defective PEPCK mRNA suppression by 0.1 and 0.5 nM insulin compared to cells cultured in control serum (p<0.0001), representing a shift to the right of the insulin dose …
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Linking Environmental Toxicant Exposure To Diabetes Susceptibility
… of the key hepatic gluconeogenic genes PEPCK and G6Pase. However, this suppression was not solely mediated by the dioxin (AhR) receptor as we observed that dioxin treatment stimulated the AMPK signaling pathway, which is known to have a suppressive effect on PEPCK gene expression.</p> …
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Engineering a Reverse C4 Photosynthetic Pathway in C3 species Arabidopsis thaliana
… Two subtypes of the C4 biochemical pathway, the PEPCK and NAD-ME subtypes were tested. A. thaliana genes most suitable for assembling these subtypes were selected based on homology to C4 genes and relative transcript abundance. Transgenic plants carrying a reverse PEPCK C4 pathway displayed no …
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Enhanced methylglyoxal formation in cystathionine γ-lyase knockout mice
… and -2, phosphoenolpyruvate carboxykinase (PEPCK), and estrogen-related receptor (ERR)α mRNA expression levels in renal extracts from 6-22 week-old CSE⁻/⁻ mice. Likewise, FBPase-1 and -2 mRNA levels were also significantly down-regulated in aorta tissues from 14-16 week-old CSE⁻/⁻ mice. …
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Impact of Dietary Carbohydrate and Protein Levels on Carbohydrate Metabolism
… decreased GNG capacity (decreased expression of PEPCK) with increased lipogenic activity (increased FAS expression) in addition to the corresponding SREBP1c expression in liver. This increase in dietary CARB then exceeded skeletal muscle utilization of glucose, becoming reliant on adipose uptake …
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Tissue enzyme changes in air-exposed American eels.
… and gluconeogenesis (GDH, AspAT, AlaAT, and PEPCK). In addition, kinetic analysis was undertaken for PFK and PK in harvested posterior kidney. Blood collected at the time of harvest was analyzed for glucose, lactate and cortisol to determine circulating levels. Muscle samples were also …
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The Search for Novel Anthelmintic Targets: Characterizing Alternative Metabolic Pathways in Caenorhabditis elegans
Around a quarter of the human population is infected by parasitic helminths which also infect and place a large economic burden on the agricultural industry. Unfortunately anthelmintic resistance is a growing problem. Helminths survive long periods of low to no oxygen in their hosts, but their …
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REGULATION OF CIRCADIAN CLOCKS AND METABOLISM BY ARYL HYDROCARBON RECEPTOR
… (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK) and key fatty acid oxidation enzymes, carnitine palmitoyl transferase1b (CPT1b) and acyl-CoA oxidase (ACO). Conversely, AhR agonists increase PPARa; expression in a BMAL1-dependent manner. In addition, AhRKO mice display altered rhythm for …
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The effect of maternal nutrition on offspring gene regulation via epigenetic modulation
… downstream phosphoenolpyruvate carboxykinase (PEPCK) gene transcription was also up-regulated in female LP pups through the increased binding of C/EBPβ at its promoter. On the other hand, GLUT4 is an insulin-regulated glucose transporter involved in insulin sensitivity and carbohydrate …