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Showing 1 to 20 of 23 for “"Pellicle"”.
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Physical and Molecular Processes Controlling the Formation, Structure and Breakdown of the Salivary Pellicle
Objectives: Despite the importance of the pellicle in oral physiological and pathological processes, an understanding of the fundamental physical and molecular mechanisms underlying the structural formation and breakdown of this protein film remains unresolved. Therefore the work carried out herein …
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Effect of irradiation of head and neck tumours on the proteins' composition of the enamel salivary pellicle
… in vivo enamel salivary pellice. For this reason pellicle was collected from irradiated and not irradiated tumour patients. The samples were analyzed by electrophoretical separation and silver-staining as well as immunodetection of alpha-amylase, IgA and acidic-PRPs. Conclusively to our results, …
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Discovery and functional analyses of new components of the apical complex and the pellicle of Toxoplasma gondii
The pellicle of the apicomplexan parasite Toxoplasma gondii is a shared structure among the supergroup Alveolata, which consists of a patchwork of flattened vesicles underneath the plasma membrane. The apical complex, from which the phylum obtains its name, is the defining feature of apicomplexans. …
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Role of salivary films in assessing astringent compounds
… proteins bound to the epithelial layer (mucosal pellicle).<br/>Studies of oral epithelial cells have been used to confirm which proteins form the mucosal pellicle. Model particles with different surface chemistries and tissue cultured cell lines have been used to determine how this pellicle …
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Investigations of the assembly and function of the Toxoplasma micropore
… characteristic features of this parasite is its pellicle, a complex three-layer membrane that includes flattened vesicles that run the entire parasite. *Toxoplasma*’s pellicle has conferred the parasite protection, cell shape, and motility, which is important for the parasite’s host invasion and …
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Characterization of a Novel Fis1 Interactor Required for Peripheral Distribution of the Mitochondrion of Toxoplasma Gondii
… periphery and is in close proximity to the pellicle‚ suggesting the presence of membrane contact sites. Upon egress‚ these contact sites disappear‚ and the mitochondrion retracts and collapses towards the apical end of the parasite. Once reinvaded‚ the lasso shape is quickly reformed‚ …
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Interaction between tin/flouride-containing solutions and artificially created dental pellicles on erosion prevetion in vitro
… their interaction with the acquired dental pellicle (ADP), a clinically relevant erosion protective factor, is not well known and needs to be investigated. OBJECTIVES: To investigate the anti-erosive properties of fluoride-containing solutions and stannous solutions on enamel and dentin …
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Characterization and control of biofilm and biofluid microstructure and rheology
… medium. This biofilm produces a thick cellulose pellicle, but instead of being harmful, its properties are actually useful for many applications for example, as a scaffold in tissue engineering. Addition of alginate changes the pellicle microstructure, making it denser in an analogous effect to …
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Ethylene response and phytohormone-mediated regulation of gene expression in Komagataeibacter xylinus ATCC 53582
… cmcAx, ccpAx and bglAx) were determined using pellicle assays and reverse transcription quantitative polymerase chain reaction (RT-qPCR), respectively. Ethylene enhanced cellulose yield by upregulating bcsA and bcsB expression, while IAA decreased cellulose yield by downregulating bcsA. …
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In vitro evaluation of equine bone-marrow derived mesenchymal stromal cells to combat orthopedic biofilm infections
… orthopedic infections, could reduce biomass, pellicle size, and live bacteria of biofilms of orthopedic infectious agents S. aureus and E. coli. Next, we investigated whether MSC could modulate immune response to S. aureus biofilms by reducing secretion of pro-inflammatory cytokines by …
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Assessment of Salmonella enterica Biofilms and Expression Differences Among Serovars
… 3); the third detailing our exploration of the pellicle forming properties of Salmonella with a focus on the lesser studied Kentucky serovar (Chapter 4); before ending with an evaluation of transcriptional dynamics of poultry isolates of different Salmonella serovars during biofilm formation …
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Immunological Characterization of Membrane Skeletal Proteins in "Tetrahymena pyriformis" GL
The cell cortex (or pellicle) of "Tetrahymena" is composed of three closely associated layers: the cell membrane, the alveolar sacs, and the epiplasm. The epiplasm is the innermost layer of the cortex and is thought to be analogous to the membrane skeleton found in eukaryotic cells, since it …
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Characterisation of the localisation and function of the Bacillus subtilis YuaB protein during biofilm formation
… in two “subtypes” of biofilm, a biofilm pellicle floating on the air-liquid interface and complex colonies formed on solid surfaces. This is achieved using a combination of biofilm fractionation combined with Western blotting and a newly developed method for immuno-fluorescent labelling …
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Basidiosporogenesis and developmental anatomy of spore release in the Russulales: a systematic interpretation
… wall layers are associated with an evanescent pellicle and four wall layers are derived from the sterigma and young spore. The amyloid portion of the spore wall in the Russulales is an electron-translucent wall layer covered by an electron-dense surface layer. Ontogeny of spore-wall …
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Poliovirus Replication: Interactions with Cytoplasmic Membranes and with Compounds Which Affect Virus Biosynthesis
… rough microsomal membranes which form a thick pellicle in the lower third of the centrifuge tube. These membranes probably constitute the intracellular location for the synthesis and incorporation of phospholipids into membranes. Uninfected cells still show this distribution of …
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The Role of the Extracellular Matrix components of Salmonella enterica serovar Typhimurium Biofilms in Biofilm Formation and Architecture via in vitro analysis
… red-Coomassie blue staining and the biomass in pellicle biofilms by crystal violet assay. Next, we utilized a computational 4D approach that was developed in collaboration with the Quiesser and Buttaro Labs to identify the contribution of each matrix component into the material properties of the …
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Cell-walls of growing plant cells
… When hydration of A. xylinus cellulose composite pellicles was reduced using PEG 6000 solution and re-hydrated in buffer solution, followed by treatment with α-expansin or snail acetone powder extract, it was found that expansin and snail powder extracts caused a rapid rehydration of the …
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Detection of energetic neutral flux emanating from extreme ultraviolet light lithography sources
… materials prevents the traditional use of a pellicle to protect the collection optics, which reflect the produced light to an intermediate focus location. The debris limits the lifetime of the collector optics, and leads to a critical increase in cost of ownership. As such there is a …
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Fabrication and characterization of a magnetic bacterial nanocellulose for neurovascular reconstruction of cerebral aneurysms
… Acetobacter xylinum, which is extruded as a pellicle in the interface liquid/air to protect the bacteria from dehydration and UV radiation. BNC possesses a multiple of desirable physical and chemical properties for tissue engineering applications such as biocompatibility, high swell ratio, …
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Biochemische Zusammensetzung der in vivo gebildeten Pellikel in verschiedenen Lokalisationen der Mundhöhle
Ziel dieser Studie war die biochemische Charakterisierung der in vivo gebildeten Pellikel in acht verschiedenen Lokalisationen der Mundhöhle. Die Pellikelgewinnung erfolgte mittels intraoral fixierter Rinderschmelzplättchen, die von fünf gesunden Probanden über Zeiten von 3, 30 und 120 min getragen …
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