Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 106 for “"PNA"”.
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Synthesis of Peptide Nucleic Acid (PNA) and Entrapment of PNA into Liposomes
Peptide Nucleic Acid (PNA) is a synthetic pseudopeptide nucleic acid analogue in which the deoxyribose phosphodiester backbone of DNA is replaced by a pseudo-peptide composed of N-(2 aminoethyl)glycine units, resulting in an achiral and uncharged DNA mimic. PNA is chemically stable and resistant to …
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PNA-protein conjugates for nano-scale modeling of protein aggregates
… In this research, the unique characteristics of PNA were applied to create controllable protein assemblies directed by precise PNA-DNA hybridization. The signatures of assembly were studied via FRET, providing a powerful tool which should be effective in live system imaging. Two model systems …
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Induktion von Sekundärstrukturen durch den Einbau von Alanyl-PNA in Peptide und Proteine
… von Aktivität nach sich ziehen. Alanyl-PNA ist ein Oligopeptid alternierender Konfiguration mit Nukleobasen in β-Position der Alanyl-Einheiten, das durch Wasserstoffbrückenbildung und π-Stacking mit einem komplementären Strang Paarungsduplexe mit β-faltblattartiger linearer Struktur …
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Neue DNA-Biosensoren mit direkter elektrochemischer Detektion auf Basis von Metall-PNA-Konjugaten
… der Basis von Konjugaten aus Peptid-Nukleinsäure(PNA) mit kovalent gebundenen Metallocenen (Ferrocen und Cobaltocenium) beschrieben. Kommerziell erhältliche DNA-Arrays sind hinsichtlich der Erkennung von Punktmutationen in den Targets limitiert, da DNA-Oligomere als Sonden über keine ausreichend …
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Design, Synthesis And Characterization Of Lipidated Pna-Peptide Conjugates As Potentialtherapeutic And Diagnostic Reagents
Peptide Nucleic Acids: PNAs), are an ideal choice for antisense and antigene tools and probes because of their stability and high binding affinity, but have limited application as therapeutic and diagnostic agents because of poor membrane permeability. To increase membrane permeability, we …
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PNA-Ligand Bioconjugates as Potential Building Blocks for Sequence-Specific, Metal-Mediated DNA-/RNA-Cleavage
… of new nuclease mimics on the basis of PNA-metal bioconjugates is described. The design of artificial enzymes for sequence-specific DNA and RNA cleavage is one of the most challenging problems in modern biotechnology, since the commercially available biotools are restricted to a limited …
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Inibizione selettiva del gene MYCN mediante PNA (acidi peptido nucleici) anti-gene nel rabdomiosarcoma umano
… it is an ideal target for an effective therapy. PNAs belong to the latest class of nucleic acid-based therapeutics, and they can bind chromosomal DNA and block gene transcription (anti-gene activity). We have developed an anti-gene PNA that targets specifically the MYCN gene to block its …
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Variation in chromosomal aberration induction in differing ATM and ASPM genotypic backgrounds detected via a modified PNA FISH technique
A modified PNA (peptide nucleic acid) FISH (fluorescence in situ hybridization) method has been developed for efficient quantitative and qualitative analysis of chromosomal aberration formation. PNA FISH was used to detect a heightened sensitivity in mouse fibroblasts in response to ionizing …
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Molecular Dynamics Study of Single Stranded Peptide Nucleic Acids
<p>A PNA molecule is a DNA strand where the sugar-phosphate backbone has been replaced by a structurally homomorphous pseudopeptide chain consisting of <em>N</em>(2-aminoethyl)-glycine units. PNA binds strongly to both DNA and RNA. However, an analysis of the X-ray and NMR data show that the …
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Part I. A Stacked Intercalator Approach to Selectively Target Mismatches Within CTG and CUG Trinucleotide Repeats. Part II. Aromatic Stacking of Naphthoyl Modified Cytosines Within PNA·DNA Duplexes
… cytosines were synthesized and incorporated into PNA monomers followed by preparation of PNA oligomers containing multiple substitutions of the modified cytosine. Thermal denaturation studies and thermodynamic analyses of resulting PNA·DNA duplexes were performed to determine the …
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Functionalization of Peptide Nucleic Acids via post-synthetic click chemistry
Peptide Nucleic Acid (PNA) has shown great potential in molecular diagnostics, antisense/antigene therapy and nanotechnology. Like other synthetic nucleic acids and artificial analogues, PNA has been extensively modified to achieve better performance in these applications. To efficiently develop …
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Synthese modifizierter Peptidnukleinsäuren und deren Anwendung zur DNA-Analyse
… wurde die katalytische Hydrolyse einer Ester-PNA durch ein Cu2+-Komplex-PNA-Konjugat untersucht, die sowohl durch Einzelstrang-DNA als auch durch Doppelstrang-DNA gesteuert werden kann. Die Hydrolyse einer Ester-PNA am Einzelstrang-DNA-Templat, die durch den Kupferkomplex einer Katalysator-PNA …
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Evaluation of the Protein Recognition Properties of Peptide Nucleic Acids
… to recognize the non-standard nucleic acid, PNA (peptide nucleic acid). PNA has immense potential in biomedical applications due to its increased thermostability and nuclease resistance over natural nucleic acids. PNA represents a superior alternative to natural nucleic acids in many …
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Characterization, toxicity, and biological activities of organometallic compounds and peptide nucleic acids for potential use as antimicrobials
… compounds (OMC) and peptide nucleic acids (PNA). Organometallic compounds show promise as antimicrobial drugs because of their structural difference from conventional antibiotics and antimicrobials, and because of the ability to "tune" their chemical and biological properties by varying …
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The characterisation of the peanut agglutinin an evolved plant lectin, with improved specificity to the Thompson Freidenriech antigen
Peanut agglutinin (PNA), a carbohydrate binding protein, is able to recognise and bind a number of distinct carbohydrate structures that have been implicated in a number of disease pathologies in humans. In vitro studies of PNA have previously been shown to have some specificity for the Thomson …
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EMISSIVE PEPTIDE NUCLEIC ACIDS (PNAS): POTENTIAL TOOLS FOR INNOVATIVE DIAGNOSTICS AND THERAPEUTICS
… and aim of the thesis Peptide nucleic acids (PNAs)1 are synthetic mimics of natural nucleic acids, in which the chiral and negatively charged sugar-phosphate backbone is replaced by achiral and neutral N-(2-aminoethyl)-glycine (aeg) units, where the four nucleobases (thymine, adenine, …
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Modifizierte Peptidnucleinsäuren für die sequenzselektive DNA-Spaltung und DNA-Nachweis
… Metallkomplex-Konjugate von Peptidnucleinsäuren (PNAs) synthetisiert und in zwei Projekten angewendet: als “künstliche Restriktionsenzyme“ für die sequenzselektive Spaltung von Einzelstrang-DNA sowie als Komponenten einer DNA-templatvermittelten Metallkatalyse. Im ersten Projekt konnte durch …
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DEVELOPMENT OF A RAPID DIAGNOSTIC ASSAY FOR THE COLORIMETRIC DETECTION OF BACTERIAL AND VIRAL PATHOGENS USING LOOP-MEDIATED ISOTHERMAL AMPLIFICATION COUPLED WITH A PEPTIDE NUCLEIC ACID AND GOLD NANOPARTICLE REPORTER SYSTEM
… reaction followed by a peptide nucleic acid (PNA) probe and gold nanoparticle (AuNP) detection system that results in a colorimetric assay. By employing modified LAMP primers, the target-dependent, co-amplification of a novel, sequence is generated, which serves as the target for PNA/AuNP …
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Pilot Scale Application of Partial Nitritation Anammox and Partial Denitrification Anammox Treating Industrial Fertilizer Waste with High Ammonia and Nitrate
… The first method, partial nitritation anammox (PNA) is very effective at removing high strength NH4 from wastewater. Although PNA is effective in removing NH4, it does not remove NO3, which is why the second method of shortcut removal incorporated in this study is partial denitrification anammox …
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Discovery of PSMA-Specific Peptide Ligands and Development of A Noncovalent Method to Attach Chemical Moieties to siRNA
… clinical applications of peptide nucleic acids (PNAs). In Chapter 3, a PSMA specific peptide ligand was identified using phage display. A novel combinatorial phage biopanning strategy was employed to identify peptide ligands, which can specifically bind to PSMA. Among all candidate peptides, GTI …
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