Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 66 for “"PDX"”.
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Emergenza clonale di resistenza al metotrexato nelle cellule PDX con codice a barre dell'osteosarcoma
… modelli di xenotrapianto derivato dal paziente (PDX), quindi abbiamo generato cloni resistenti trattando le linee cellulari PDX con codice a barre con singolo agente Metotrexato (MTX). Per ogni clone è stato estratto il DNA al fine di eseguire il sequenziamento del DNA codificato a barre per …
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Developing canine osteosarcoma and human non-small cell lung cancer PDX models to study immunotherapies targeting canine and human T cells
In the last decade, therapies targeting one's own immune system (immunotherapies) have proven to be effective in extending the quality of life and lifespan for a variety of patients with lung, breast, and prostate cancer. However, patients where their cancer is either unresponsive or become …
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Dysfunction of Pancreatic INS-1 Beta-Cells Induced by Chronic Exposure to High Glucose
… 노출되었을 때 인슐린 유전자 발현과 그의 전사에 중요한 전사인자들 중의 하나인 pdx-1 유전자 발현이 억제되는 데, 이와 관련된 기작을 연구하고자 하였다. 대상 및 방법: 고농도 포도당의 지속적인 노출에 의하여 베타세포의 중요한 기능 중 하나인 인슐린 유전자 발현이 감소하는 지를 확인하기 위해, 백서의 소도세포와 세포주, 마우스 세포주를 대상으로 RT-PCR 방법을 이용하여 인슐린 및 주요 전사인자의 유전자 발현을 확인하였다. 이후 고농도 포도당에 의한 전사인자, 특히 pdx-1 의 발현 감소가 어떤 단계의 …
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Phenotypic analysis of human stem cells and their differentiation potential towards pancreatic cells
… dedifferentiated into PCMOs expressed SOX-17 and PDX-1, in addition to the monocytic markers CD14 and CD68. A subset of monocytes dedifferentiated into f-MOs expressed PDX-1, while the remaining cells expressed CD14 indicating the presence of two different cell populations. Therefore it appeared …
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A Context-Forward In Vivo Functional Genomics Platform For Target Discovery and Establishing Vulnerability Context In Pancreatic Cancer
… expand on PDAC surface protein dependencies in PDX cell lines. In parallel, leveraging our internally established set of over 50 PDX models, we generated a PDAC co-expression network (PCEN) of dynamically expressed genes to inform on transcriptional signatures underlying disease diversity. Upon …
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An Investigation into the Vascular Phenotypes of Breast Patient- Derived Xenografts
… use of breast cancer patient-derived xenografts (PDXs) in PAI, to begin to answer the aforementioned questions in clinically-relevant models. However, whether PDXs are good vascular models themselves remains unknown. This thesis conducts a careful evaluation of whether vascular phenotypes differ …
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Modulation of Pi3K-Akt Pathway In Colorectal Cancer
… patient derived xenografts (PDX) treated with MK2206 or carrier, and (3) MK2206-treated cell lines. PD inhibition was deep in cell lines, less but significant in PDX, and only modest in patients. Expression of several receptor tyrosine kinases (RTKs) increased after AKT …
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Investigation of metabolism in glioblastoma patient-derived xenografts using deuterium metabolic imaging
… metabolism in patient-derived xenografts (PDX) of glioblastoma in mice and rats respectively. Using 2H-labelled glucose, I showed that despite exhibiting the same concentration of glucose within the tumour, glycolytic PDX subtypes produced more labelled lactate and mitochondrial PDX …
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Bioorganic activation of cytochrome P-450cam
… electron transport partner, putidaredoxin (Pdx), is determined using a combination of site-directed mutagenesis and EPR spectroscopy. The identity of the rapidly reacting cysteine in P-450cam, which has been used as an attachment site for probes of Pdx-P450cam interaction, is identified. The …
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Investigating the Genomics and Heterogeneity of Childhood Non-Hodgkin Lymphoma
… a novel relapsed BL patient-derived xenograft (PDX) was established---to our knowledge the first described in the literature---and the maintenance of genetic ITH through engraftment in immune-compromised mice was demonstrated through whole genome sequencing (WES). In addition, pervasive genetic …
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Tracking Treatment Response and Resistance to Parp Inhibition (Talazoparib) In Hereditary Pancreatic Cancer.
… in pancreatic patient-derived xenograft (PDX) tumors; and 2. The use of shRNA technology on pancreatic PDX tumors can elucidate synthetic lethal partners to overcome therapeutic resistance to PARP inhibition. To test this hypothesis, I set out to first further characterize the hereditary …
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APPLICATION OF STRUCTURAL, MOLECULAR, AND PROTOEMIC STUDIES TO INVESTIGATE ON-TARGET AND OFF-TARGET MECHANISMS OF LORLATINIB RESISTANCE IN ALK DRIVEN NEUROBLASTOMA
… assays and patient derived xenograft models (PDX) to uncover mechanism of on-target resistance caused by acquired secondary mutations in ALK kinase domain. In the third chapter, I describe how we applied transcriptomic and proteomics methods to elucidate potential mechanism of ALK independent, …
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The role of N-cadherin and S1P/S1P1 in pancreas development
… pancreatic bud due to decreased proliferation of Pdx1+ cells. Other epithelial organs revealed growth defects. The lung epithelium had fewer branches and the stomach was smaller. This suggests that endothelial cells are essential for early development of the pancreatic epithelium. Since N-cadherin …
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MECHANOPROPERTIES, HETEROGENEITY AND CELL MIGRATION IN GLIOBLASTOMA
… white matter tracks: Patient-Derived Xenograft (PDX) has been a standard to reproduce them in order to study GBM invasion. However, PDX presents many disadvantages, including time consumption, hard standardization, high cost and ethical concerns. The present PhD thesis report aims at summarizing …
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Targeting Protein Degradation to Uncover Novel Oncoprotein Drivers of Acute Leukemia
… in a panel of patient derived ALL xenografts (PDX) harboring rearrangement of IGH-CRLF2, EPOR, ATF7IP-JAK2 ex vivo. All the tested tumors were highly sensitive to SJ6986 with IC50 at nanomolar range. PK analyses in mice indicated rapid absorption and over 80% oral bioavailability for SJ6986. PD …
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Developing Targeted Therapies for T-cell Acute Lymphoblastic Leukemia
… & PD) studies using a patient-derived xenograft (PDX) model to predict the achievable drug exposure and lymphocyte-specific protein tyrosine kinase (LCK) inhibition in human. Mouse dasatinib exposure was measured by liquid chromatography–mass spectrometry (LC-MS), and LCK inhibition was quantified …
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Cambios histomorfométricos tardíos del islote pancreático en un modelo murino, sometido a cirugía bariátrica/metabólica experimental bypass gástrico con y de roux
… masa beta y alfa; factores de transcripción (Pdx-1 y ARX), proliferación (Ki67). Además comprobamos los cambios histológicos y funcionales adaptativos en el asa alimentaria, así como transportadores intestinales de glucosa y otros glúcidos. Resultados: Nuestro estudio mostró un incremento …
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Quantitative analysis of signaling networks in proneural glioblastoma
… in GBM, we treated patientderived xenograft (PDX) cell lines with MK-₁₇₇₅. The response was more heterogeneous, but we measured decreased CDK₁ phosphorylation, increased DNA damage, and death by apoptosis. These results were validated in a flank GBM PDX model where treatment with MK-₁₇₇₅ …
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Head-dependent modelling and optimisation of water distribution systems
… pressure-dependent extension of EPANET (EPANET-PDX) that has an embedded logistic nodal head-flow function. The novelty of the proposed PDA is the formulation of a new, more efficient and robust implementation of the line search and backtracking procedure that greatly enhances computational …
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