Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Neutrophil Migration"”.
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The role of chemokine receptor trafficking in regulating neutrophil migration to inflammatory sites
Neutrophils are the first immune cells to be recruited to sites of tissue injury or infection. Upon detection of an inflammatory stimulus, neutrophils exit the vasculature and migrate directionally through the interstitial tissue towards the target site. Once at the target site, neutrophils may …
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Understanding the Effect of BVDV on Innate Immune Response of Neutrophils
… has been done to determine the effect of BVDV on neutrophils. Neutrophils are one of the most abundant while blood cells (WBC) in the peripheral blood, which play a critical role in the innate as well as adaptive immune response. The current study measured the effect of BVDV infection on viability …
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Molecular signaling programs underlying neutrophil polarity and chemotaxis
Neutrophils adopt a highly polarized morphology and integrate many matrix-specific signals to undergo specialized migratory events to pursue invading pathogens. While these matrix-specific signaling events have emerged as crucial elements for proper migration and recruitment of neutrophil cells, …
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LL-37 and citrullinated LL-37: differential modulation of the oxylipin - chemokine axis
… analysis by qRT-PCR, and impact of peptide on neutrophil migration was examined in a transwell assay using neutrophils isolated from human blood. LL-37 significantly enhanced oxylipins such as PGE2, 11- and 15-HETE, 15-HETrE, and cytochrome P450-derived 9,10- and 12,13-EpOME. Whereas citLL-37 …
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Identification of the Role of P-Rex1 and its Adaptor Functions in Neutrophils
… GTPase Rac, recognised for its involvement in neutrophil migration and ROS generation in response to GPCR activation—functions traditionally attributed to its Rac-GEF activity. Additionally, previous findings from our laboratory demonstrated a GEF-independent role for P-Rex1 in GPCR …
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Quantitative Image Analysis in the Study of Neutrophil and Neutrophil-Like HL-60 Chemotaxis
Neutrophils are fast-moving first responders of the innate immune system. External chemoattractant signals result in neutrophil polarization: the neutrophil forms a leading edge (front) which constantly protrudes and retracts actin-rich pseudopods, and a contractile myosin-enriched trailing edge …
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Characterizing physiological and genetic differences in the early immune response to Haemonchus contortus in resistant and susceptible sheep
… 3, 5, and 7 d after infection with H. contortus. Neutrophil migration to abomasal mucosa and lymph node development were higher in hair sheep than in wool sheep. Gene expression analysis indicated no difference in the abomasal lymph node as both breeds expressed a general T-helper cell type 2 …
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Treatment of Ischemic Equine Jejunum with Topical and Intraluminal Carolina Rinse
… in decreasing vascular permeability and neutrophil infiltration in reperfused equine small intestine. The objective of this study was to show that CRS applied topically and intraluminally could prevent immediate reperfusion injury after low flow ischemia or distention in the equine …
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Analysis of Cellular Transcriptomic Changes Induced by Merkel Cell Polyomavirus miRNA
… the cytokine stimulating gene, SP100, and the neutrophil stimulator chemokine, CXCL8. Moreover, dual luciferase assays revealed that SP100 and MAPK10 (a member of mitogen-activated protein kinases (MAPK) family which is involved in regulation of CXCL8 expression) are directly and specifically …
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Oxidative stress and inflammation as a response to glucose exposure and dialysis
… cytokines. A transwell model was used to analyse neutrophil migration across endothelial cell layer. This thesis also had a clinical approach, looking at inflammation, oxidative stress and AGE formation, in combination with trace elements in diabetic- and non-diabetic dialysis patients. All …
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Aging Effects on Acute Lung Inflammation After Burn Injury
… given a comparable sized wound. We found that neutrophils continue to accumulate in the lungs of aged mice at 24 hours after burn injury, but do not migrate into the alveoli. We also showed that neutrophil migration towards KC--a dominant neutrophil chemokine--in a transwell chemotaxis assay is …
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Investigation of Neutrophil-Like HL-60 Cell Migration in a 3D Collagen Matrix
<p>It is known that cell migration in innate and adaptive immune system plays a fundamental role in human health. Among the immune cells, neutrophils are one of the most essential cells in protecting the body against invading pathogens. In the presence of chemoattractants, these cells are the first …
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Investigating the role of primary cilia loss in intrahepatic cholangiocarcinoma
… Cxcl10 and Cxcl11. However, the expression of neutrophil-specific chemokines such as Cxcl1, Cxcl2 and Cxcl5 was significantly suppressed. In concordance, immunohistochemical analysis revealed that neutrophil infiltration was frequently decreased in the livers of mice with non-ciliated biliary …
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The central role of the neutrophil in ischaemic/reperfusion injury
… the accumulation and inappropriate activation of neutrophils which occurs when the tissue is reperfused. To test this hypothesis a number of in vitro models of the sequential stages of ischaemia/reperfusion injury were examined. Methods were initially developed to examine the adhesion of …
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Microfluidic platforms for studying cell behavior in complex microenvironments
… and applying microfluidic platforms to study (1) neutrophil chemotaxis, (2) intercellular communication, and (3) cell behavior under controlled oxygen conditions. In chapter 1, conventional methods and emerging techniques using microfluidic platforms to study cell behavior in complex …
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Determining the contribution of formylated peptides and formyl peptide receptor 1 to the pathogenesis of acute lung injury
Neutrophils as key effector cells of the innate immune system migrate from the circulation into sites of inflammation and are essential for the containment, killing and clearance of invading pathogens through a variety of highly regulated cell functions. Despite this beneficial role their …
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Targeted Delivery of Surface Modified Nanoparticles: Modulation of Inflammation for Acute Lung Injury
… and endothelial cells and III) leukocyte migration from blood circulation into alveoli. Currently, there is no FDA approved pharmacological treatment available except the supportive mechanical ventilation therapy. Numerous clinical trials involving pharmacological therapies aimed at …
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Investigating the Role of Chemokines in Neutrophil Recruitment and Resolution During Inflammation
Neutrophils are innate immune cells and the first to be recruited to sites of injury, where they are essential for host defence. Defective neutrophil recruitment can inhibit pathogen clearance, while impaired neutrophil resolution can lead to chronic inflammation. Therefore, it is essential that …
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Structural Basis Of Epigenetic Regulation And Protein Scaffolding In Development And Diseases
… in both inflammation and pancreatic cancers. In neutrophils, this NHERF1-mediated macromolecular complex is essential in intracellular calcium mobilization and neutrophil migration. In pancreatic cancer cells, this complex regulates tumor proliferation and invasion. Therefore, targeting this …