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Showing 1 to 20 of 34 for “"Mutational studies"”.
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Kinetic and mutational studies of two RNA-interacting enzymes
Cellular enzymes interact with RNA substrates at several key stages in an organism's life cycle, particularly during the processes of transcription and translation. A more detailed description of enzyme mechanistic features and kinetic parameters is important both for understanding molecular …
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Mutational studies of the cytochrome bc(1) complex of Rhodobacter sphaeroides
The cytochrome $bc\sb1$ complex is a multisubunit integral membrane protein. This enzyme is a central component in electron transport where it oxidizes quinol while forming a transmembrane proton gradient. The cytochrome b subunit is the locus of two distinct quinol binding sites as well as the …
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Mutational studies in the dNTP binding pocket of human immunodeficiency virus type 1 reverse transcriptase
It was earlier postulated that Gln 91 of HIV-1 RT stabilizes the side chain of Tyr 183 via hydrogen bonding interaction between 0(H) of Y1 83 and CO of Q91 (Harris et al., BIOCHEMISTRY 37: 9630, 1998). In an attempt to understand the function of Gln 91 in the catalytic mechanism, mutants of this …
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Structure and Function of Colicin E5 Ribonuclease Domain
… emissions. The isothermal titration calorimetry studies showed a -10 kcal/mol enthalpy change upon the formation of ColE5•ImmE5 complex. The mutational studies of ImmE5 indicated that ImmE5 K4 and D95 contributes a larger inhibitory effect toward ColE5. The structure comparison of ColE5 in …
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Structural Studies On The Rubella Virus Capsid Protein And Its Organization In The Virion
… tomograms of rubella virus particles and mutational studies on the capsid protein, provides a low resolution structure of the virus particle. Nucleocapsid assembly studies on the rubella capsid protein has given additional information on the factors affecting formation of the virion cores. …
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Characterization of the expression patterns of <i>CELLULOSE SYNTHASE-LIKE A (CSLA)</i> genes in <i>arabidopsis thaliana</i> using reporter promoter-fusions and immunolocalization
… insights into possible focal regions for future mutational studies.</p>
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Biochemical and Structural Studies of Pseudouridine 55 Synthase
… information from structural, biochemical and mutational studies allows us to propose the likely role of the conserved tyrosine as a general base for proton abstraction in PsiS-catalyzed reaction as well as structural role of the hydrophobic phenol ring in the active site.
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Towards Establishing Methods of Synthesis, Purification, and Structural Determination for Human Fibrinogen
… link in these various pathophysiological states. Studies focused on improving expression systems for recombinant fibrinogen, and developing more rapid and robust techniques for purifying fibrinogen from complex media would provide a means by which to progress mutational studies of fibrinogen. …
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The role of buried stack residues in the folding of the beta-helix domain of P22 tailspike
… a [beta]-helical fold, we performed systematic mutational studies using the complete P22 tailspike protein, which contains a 13 rung [beta]-helix domain. The in vivo folding and assembly of 150 single mutant polypeptide chains were characterized at multiple temperatures by SDS-PAGE, which …
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EPIGENETIC MODIFICATIONS REGULATING LIVER HOMEOSTASIS AND TUMORIGENESIS
… remodeling complex have been commonly reported. Mutational studies clearly indicated a role for epigenetic modifications in HCC development. However, it is still unclear whether SWI/SNF mutations have a role in tumor formation. Our data suggest a new role of Arid1A as one of the key players in …
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Characterization of The Agrobacterium Tumefaciens Virb2 Pilin of The Virb/D4 Type Iv Secretion System
… pathway involving the VirB2 pilin. Topology studies demonstrated that VirB2 integrates into the inner membrane with two transmembrane regions, a small cytoplasmic loop, and a long periplasmic loop comprised of covalently linked N and C termini. VirB2 was shown by the substituted cysteine …
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Investigation of the Lacticin 481 Synthetase and Its Substrate LctA in the Production of the Antibiotic Lacticin 481
… of the lantibiotic lacticin 481. Previous studies in our laboratory revealed the protein responsible for the creation of the intricate polycyclic antimicrobial agent was tolerant of several point mutants within its peptide substrate LctA. In an effort to better understand the substrate …
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Genetic analysis of RNA polymerase/transcriptional activator interactions at thepepT promoter of S. typhimurium
… we have characterized the pepT promoter region. Mutational studies and primer extension analysis demonstrate that pepT is transcribed from two promoters, an FNR-dependent, anaerobically induced promoter (P1), and a constitutive promoter (P2) conferring low level expression. A mutant P1 promoter …
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Unraveling the Molecular Mechanisms of Munc18 and Munc13 in Mast Cell Exocytosis
… an essential role in mast cell degranulation; mutational studies revealed that point mutations in C2 domains of Munc13-4 dramatically alter Ca2+-sensitivity of degranulation, whereas absence of Munc13-4 or multiple mutations in C2 domains result in almost complete loss of exocytosis from mast …
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Biochemical Characterization of the Activity and Specificity of ULP1 Family Members
… is unable to process mammalian SUMOs. Enzymatic studies further demonstrated that XopD functions as a cysteine protease. Isopeptide bond cleavage was demonstrated by the ability of XopD to cleave the plant SUMO moiety from in vitro SUMOylated RanGAP. These studies showed that XopD has a rigid …
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Mechanistic studies of nitrile hydratases and their activator proteins
… role in the Co-type and Fe-type NHases. Mutational studies of this residue provide the first evidence of the active site Ser's involvement in metalation and maturation, as well as its impact on the catalytic mechanism. Further, this dissertation presents the first characterization of a …
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Olfactory-related receptors : methods towards enabling structural and functional studies
… expression can be used for GPCR production and mutational studies. To demonstrate this, six variants of mOR103-15 with single amino acid substitutions were expressed. Ligand-binding measurements indicated which residues were involved in ligand recognition. The choice of detergent used in the …
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Laser-assisted single-molecule refolding
… cooperativity, and the enthalpy change. Mutational studies have allowed dissection of the contribution of base pairs to kissing complex stability. And the ratio of the competing reaction pathways was determined. </p> <p>LASR experiments designed to the study of the origin of the memory …
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Electron gating and mechanism of proton exit in quinol oxidation in cyt bc1 complex from R. sphaeroides
… of quinol oxidation at the Qo-site. Previous studies showed that mutation at this position resulted to a substantially inhibited electron transfer, while the bypass rates were equal to or less than the wild-type strain. In addition, these strains showed resistance to stigmatellin and a modest …
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The Influenza A Virus NS1 Protein and Vriral mRNA Nuclear Export
… required for efficient segment 7 nuclear export. Mutational studies on NS1 were carried out to unveil the mechanistic role of this protein in viral mRNA nuclear export, by plasmid transfection as well as in the context of recombinant viruses. These approaches indicated that both functional domains …
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