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Showing 1 to 20 of 38 for “"Mutase"”.
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Studies on Methylmalonyl-CoA Mutase from Escherichia coli
Methylmalonyl-CoA mutase (MCM, E.C. 5.4.99.2), a coenzyme B12-dependent enzyme, catalyses the inter conversion of succinyl-CoA and methylmalonyl-CoA. The gene (sbm) encoding this enzyme is found in Escherichia coli (E. coli) at 62.3min on the E. coli chromosome. However, the metabolic role of this …
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Yeast Chorismate Mutase: Molecular Evolution of an Allosteric Enzyme
Die Chorismatmutase (CM, EC 5.4.99.5), kodiert durch ARO7, katalysiert die Claisen-Umlagerung von Chorismat zu Prephenat in der Biosynthese von Tyrosin und Phenylalanin. Das relativ kleine, dimere Enzym der Hefe Saccharomyces cerevisiae wird allosterisch durch Tryptophan aktiviert und allosterisch …
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Mechanistic studies on chorismate mutase-prephenate dehydrogenase from E. coli
Chorismate mutase-prephenate dehydrogenase is a bifunctional enzyme that catalyzes two sequential reactions in the biosynthesis of tyrosine in E. coli and other enteric bacteria. Chorismate mutase catalyzes the pericyclic rearrangement of chorismate to prephenate which is subsequently converted to …
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Syntheses of chorismate analogs for investigation of structural requirements for chorismate mutase
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1994.
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An Investigation of Acetobacter aceti N5-Carboxyaminoimidazole Ribonucleotide Mutase and Its purE-purK Operon
… enzyme N5-carboxyaminoimidazole ribonucleotide mutase: PurE) catalyzes the reversible intramolecular transfer of the carboxylate of N5-carboxyaminoimidazole ribonucleotide: N5-CAIR) to the C-4 position of the imidazole ring to form 4-carboxyaminoimidazole ribonucleotide: CAIR). A series of …
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Yeast cell wall proteomics: a tale of two proteins.
… Two such proteins were found, phosphoglycerate mutase and Hsp 12. Studies on these proteins are reported in chapters 2 (phosphoglycerate mutase) and chapter 3 (Hsp 12).
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Investigating cofactor transfer for a B₁₂-dependent enzyme
… one cognate enzyme in humans: methylmalonyl-CoA mutase (MCM), which is involved in the catabolism of several amino acids, cholesterol, and odd-chain fatty acids. MCM relies on two other proteins, a G-protein metallochaperone called methylmalonic aciduria type A protein (MMAA) and a protein called …
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Structural investigations of adenosylcobalamin-dependent enzyme maturation
… focuses on the maturation of methylmalonyl-CoA mutase (MCM). In humans, MCM is the only known adenosylcobalamin (AdoCbl)- dependent enzyme; mutations or deletions of MCM or any metallochaperones involved in its maturation lead to methylmalonic aciduria, an inborn error of metabolism. The final …
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Mutationsanalyse bei 21 mut°-Patienten mit Methylmalonylazidurie (MMA)
… durch einen Defekt der Methylmalonyl-CoA Mutase (MCM). In dieser Arbeit wurden 21 Patienten mit der mut°-Form auf Mutationen im MCM-Gen untersucht. Es wurden elf neue und zwei bereits beschriebene Mutationen nachgewieden und Rückschlüsse auf die Auswirkungen in der Enzymstruktur der MCM …
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Molecular characterization of phosphonate biosynthesis in nature
… is isomerized to phosphonopyruvate (PnPy) by PEP mutase (PepM). By using the pepM gene as a molecular marker for phosphonate biosynthetic capacity, I showed that phosphonate biosynthesis is both common and diverse across a wide range of environments, with pepM homologs detected in ~5% of sequenced …
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Metabolic control of the ethylmalonyl-CoA pathway in Methylobacterium extorquens AM1
… From these experiments, ethylmalonyl-CoA mutase was identified as a metabolic control point. To confirm the role of this enzyme as a metabolic control point, over-expression of ethylmalonyl-CoA mutase overrode the control and caused a change in growth phenotype. In this thesis, I …
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An old cofactor in a new light : how nature handles and repurposes adenosylcobalamin
… between AdoCbl-dependent isobutyryl-coenzyme A mutase and a G-protein metallochaperone that mediates AdoCbl delivery. Structures determined in the absence and presence of AdoCbl depict large-scale conformational changes mediated by the metallochaperone that expose the mutase active site and …
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Methods for the Study of Galactofuranose in Mycobacteria
… the mycobacterial galactan utilizing the sugar mutase UDP-galactopyranose mutase. We used this method to generate up to 10 milligrams of polymer and demonstrated that it could be selectively functionalized. Second, we leveraged the rapidly expanding set of biosynthetic probes of the …
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Konstruktion eines bakteriellen Systems zum Export von Coenzym B<sub>12</sub>
… Untereinheit (MutB) der Methylmalonyl-CoA-Mutase aus E. coli fusioniert. Zur Detektion der gebildeten Fusionsproteine wurden an das 3`-Ende der Gene die Sequenz für einen His6- oder Strep-Tag angefügt. Western-Blot-Analysen zeigten nur eine geringe Produktion des sigTorA-IF-Fusionsproteins, …
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Investigations into the biosynthesis and mode of action of the phosphonate antibiotic dehydrophos
… for the presence of the phosphoenolpyruvate mutase gene, which is required for biosynthesis of most phosphonates. Integration of one such fosmid clone into the chromosome of Streptomyces lividans led to heterologous production of dehydrophos. Deletion analysis of this clone allowed …
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Roles of the Methylcitrate and Methylmalonyl-COA Pathways in Mycobacterial Metabolism and Pathogenesis
… vitamin B12 cofactor of the methylmalonyl-CoA mutase. This route may buffer the loss of the methylcitrate cycle in vivo, depending on B12 availability or production in the host. The work here examines the relative use of the methylcitrate cycle and methylmalonyl-CoA pathways in M. tuberculosis …
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The tyrosine regulated DAHP synthase and the biosynthetic pathway of aromatic amino acids in Saccharomyces cerevisiae
… unregulierten und konstitutiv aktiven Chorismat-Mutase Aktivität, der zusätzlich defekt im Transkriptionsnetzwerk der Allgemeinen Kontrolle der Aminosäurebiosynthese ist (ARO7c, gcn4), ist im Wachstum beeinträchtigt, wenn Phenylalanin ins Kultivierungsmedium gegeben worden ist. Dieser sogenannte …
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