Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 9 of 9 for “"MshB"”.
-
Identification of native co-factors of MshB and MCA from Mycobacterium species
… involved in this pathway, such as a deacetylase (MshB) and mycothiol S-conjugate amidase (MCA). MshB catalyzes the deacetylation of GlcNAc-Ins to form GlcN-Ins and acetate. Mycothiol S-conjugate amidase (MCA) cleaves the amide bond of mycothiol S-conjugates of various drugs and toxins. The …
-
Stereoselective synthesis 2-C-alkylglucosides, potential inhibitors of mycobacterial MshB and related enzymes
… proves the urgency of the current situation. The MshB reducing agent exclusively in the actinomycetes is used as a model for the development of new anti-TB drugs. It was shown that the stereoselectivity synthesis of C-2 alkyl glucoside gave a key intermediate for the suitable synthesis of glycosyl …
-
Substrate binding to MshB, a zinc-dependent deacetylase in the mycothiol biosynthetic pathway of Mycobacterium tuberculosis
… thiol, mycothiol (MSH), is used instead. MshB catalyses the third step in the mycothiol biosynthetic pathway which involves the deacetylation of 1-O-(2-acetamido-2-deoxy-α-D-glucopyranosyl)-D-myo-inositol (GlcNAcIns), via cleavage of an amide bond, to …
-
Enzymatic Characterization of N-Acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside Deacetylase (MshB)
… a potential drug target. The deacetylase MshB catalyzes the committed step in MSH biosynthesis by converting N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside (GlcNAc-Ins) to 1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside (GlcN-Ins). In this dissertation, we …
-
Rationalising the inhibition of M. tuberculosis MshB by a series of inhibitors constructed from plumbagin conjugated via a viariable alkyl linker to a phenyl thioglycoside
… Targeting the mycothiol redox cycle has led to MshB inhibition by a series of substrate analogues. Kinetics studied showed that the competitive inhibition increased when the alkyl linker was lengthened. The binding of the inhibitors was investigated using computational techniques in order to …
-
Helical reconstruction of Mycobacterium smegmatis Mycothiol S-conjugate amidase filaments
… (Mca). Mca is similar to a biosynthetic enzyme MshB, which has partial overlapping substrate activity and is the closest homologue to Mca with a known structure. The basis for the substrate specificity differences in Mca and MshB is not well understood. Several regions of low sequence similarity …
-
New synthetic routes to functionalized 2-C-alkylglucosides, precursors of potential inhibitors of mycothiol biosynthesis in the Mycobacteria
… are either substrate-mimics or inhibitors of MshB, a N-deacetylase involved in the biosynthesis of mycothiol in the Mycobacteria, including M. tuberculosis, the causative agent of TB. Mycothiol is a low molecular weight thiol produced by Mycobacteria as a defense against oxidative stress and …
-
Synthesis of side-chain-modified mycothiol analogues incorporating carbazole quinones, and evaluation as inhibitors of enzymes in the Mycobacteria
… have significant inhibitory activity against mshB and mca in the biosynthetic pathway. These findings, together with the independent discovery of anti-TB activity associated with carbazole quinones such as B, led to the design and synthesis of a new class of hybrid molecules C which have the …
-
Viscoelastic and nanomechanical behaviors of polymeric materials
… mechanical spectral hole burning (Strain_MSHB) technique is successfully expanded to stress-controlled (Stress_MSHB) fashion. The dynamic heterogeneity of a polymer solution probed with both MSHB techniques are compared. In addition, the local temperature changes, which are thought to be …