Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"McpB"”.
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Behavioral Adaptation in Bacillus Subtilis: The Role of Selective Methylation Changes and Heterologous Receptors in Mcpb-Mediated Signaling
… study was performed on strains expressing mutant McpB proteins (with aspartates substituted at the sites of methylation) in genetic backgrounds missing all ten receptors, five receptors, or only the native copy of mcpB. These aspartate substitutions ensure a negative charge at the altered sites. …
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Transmembrane Signaling in Bacillus Subtilis Chemotaxis: Determination of the Packing Organization and Structural Changes of McpB in Response to Ligand
"In Chapter 5, we investigated the complementation and activity of a methylesterase phosphorylation point mutant, C-terminal truncated mutant, and a serine active site mutant in vivo to the attractant asparagine to determine the role CheB phosphorylation plays in regulation of receptor …
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Regulation and Role of Methylation During Chemotaxis in Bacillus Subtilis
… for either methylation or demethylation of McpB. Their role in chemotaxis is unknown but may function as structural proteins facilitating a higher-order organization of the MCPs as has been shown to exist in Escherichia coli.
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Non-canonical sensing mechanisms in bacteria
… pH chemotaxis is bidirectional in B. subtilis. McpB and its three paralogs, namely McpA, TlpA and TlpB are responsible for pH sensing. We investigated the molecular basis for bipolar pH sensing. Modified capillary assay was used to measure responses to opposite pH gradients. Through in vivo …
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Biochemistry and regulation of CheA in Bacillus subtilis chemotaxis
… increase the activity of CheA. Asparagine-bound McpB has been shown here to increase CheA activity in vitro, the first example of activation of a two-component signal transducing kinase by its proposed environmental ligand to date. Studies on the enteric chemotaxis system revealed that taxis …
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Probing surface protein patterning in biological systems using fluorescence nanoscopy
… we observed reorganization in arrays of McpB receptor proteins, key chemotactic receptors for asparagine sensing, upon exposure to saturating stimulant concentrations. More specifically, receptors formed large polar clusters in the absence of stimulant but shifted towards smaller and more …
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Biochemical and molecular characterization of methyl-accepting chemotaxis proteins from Bacillus subtilis
… of these transducer genes, designated mcpA and mcpB, reveals specific chemotactic defects towards sugars or amino acids. Despite strong homology, the two additional genes do not appear to be involved in chemotaxis. Based upon the morphological phenotype of these mutant bacteria, it is speculated …