Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"LINE-1"”.
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A comprehensive analysis of recently integrated human LINE-1 mobile elements
Long INterspersed Elements (LINE or L1) have had an enormous influence on human genomic structure, comprising about 20% of the mass of the human genome. In this analysis the most recent L1 insertions in the human genome belonging to L1Hs Ta and preTa subfamilies were examined to further understand …
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Fanconi Anaemia DNA crosslink repair factors protect against LINE-1 retrotransposition during mammalian development
Long interspersed nuclear element 1 (LINE-1) is the only autonomous retrotransposon in humans and represents 17% of the genome. LINE-1 retrotransposition occurs by a “copy and paste” mechanism in which an RNA intermediate is reverse-transcribed and integrates at a new genomic location. LINE-1 …
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Non-Parametric Analyses of the Regulatory Roles of LINE-1 Retrotransposons during Motor Neuron Differentiation
Background: Repetitive elements make up a large portion of eukaryotic genomes, constituting two-thirds of human genome. Although their functional importance were recognized as early as 1950s, study of their functions is stagnated despite the advancements in next-generation sequencing due to …
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THE ROLE OF RETROTRANSPOSITION AND EXOSOMAL DNA IN RADIATION-INDUCED GENOMIC INSTABILITY
… possible that the random insertion nature of the LINE-1 retrotransposons could be a cause of RIGI. The work in this thesis has aimed investigate the role of retrotransposable elements in the mechanism of RIGI. Three different cell lines (HF19, Caco-2, and MCF7) were used to investigate if …
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Observing and quantifying transposable element activity inside living cells
… that the activity of the human retrotransposon LINE-1 is lethal to bacterial cell growth, and propose that transposable element activity may have played a role in the early evolution of eukaryotes. Again, by quantifying how LINE-1 expression decreases bacterial growth rate, we generated a model …
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Investigating the regulation of APOBEC mutagenesis in cancer
… activity might be driven by the activity of LINE-1 retrotransposons. Firstly, the thesis details experiments to investigate APOBEC activity in cultured cancer cells. The experiments conducted suggest that p53 inactivation leads to the upregulation of LINE-1 and APOBEC3B, and that their …
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Transcriptional and Post-Transcriptional Regulation of Retroelements
… RNAs, specifically those derived from LINE-1, are oncogenic, we developed a CRISPR-based programmable RNase platform to eliminate LINE-1 RNAs. While this platform was effective against reporters, it did not perform well against endogenous LINE-1 RNAs. In the process, however, we made a …
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Restriction of Retrotransposition and Retroviral Infection by RNase L and APOBEC3G
… impaired retrotransposition of both human LINE-1 and mouse IAP elements (Chapter 2). Depletion of endogenous RNase L with siRNA increased levels of LINE-1 retrotransposition. Wild type RNase L, but not RNase L R667A, dramatically reduced L1 RNA accumulation, expression of both L1 open …
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Measurements and analyses of transposable element activity inside living cells
… genome a long interspersed nuclear element, LINE-1, one of the most prevalent and active transposable elements in humans. We introduce LINE-1 into the bacteria Escherichia coli (E. coli) and Bacillus subtilis (B. subtilis) and demonstrate that it is active in the bacteria and successfully …
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Transposable Elements in Neural Progenitor Cells
… during evolution. A transposable element called Line-1 was found to be active in neural progenitor cells and in the brain. Several studies report Line-1 transcription and frequent retrotransposition during normal brain development, with further evidence that Line-1 induced retrotransposition can …
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Diet, genetic and epigenetic signatures in women of childbearing age from a Mediterranean population: perspectives for public health
… by a validated food frequency questionnaire. LINE-1 methylation level in leukocytes was measured by pyrosequencing. Women reported poor adherence to MD (mean value 4.1) and high prevalence of folate deficiency (83.1%). Mean level of LINE-1 methylation was 65.3%. A strong association between …
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Elucidating the Genetic Basis of Canine Progressive Retinal Atrophies in Several Breeds of Dog
… revealed a long interspersed nuclear element-1 (LINE-1) insertion in the predicted promoter region of the retinal candidate gene, IMPG2. Validation of the LINE-1 insertion determined it segregated with disease and was likely to be private to LA dogs. Comprehensive WGS analyses alone were utilised …
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DNA methylation landscape in retinal degenerative diseases: implications for Public Health at the crossroad between genes and diet
… - modulate DNMTs and SIRT1 functions, affecting LINE-1 methylation levels in retinal cells. However, what we have seen in retinal cells differed from that observed in peripheral blood of AMD patients. Thus, further research - which takes into account genetic susceptibility - is needed to …
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Influência de escavações nos recalques em edificações vizinhas
… of the construction of several sections of Line 1 of the Rio de Janeiro subway are presented. A study of settlement follows, where its main causes are discussed, as well as the effects of the methodology of excavation and installation of the bracing to support the walls. The analysis was …
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Web-based network management configuration for the Indonesian Eastern Fleet Wide Area Network
… area network design using ISDN 128 Kbps and T1 line 1. 544 Mbps has been proved in this thesis by executing tests and simulation runs using EXTENDv4 software simulation program.
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GENOMIC AND EPIGENETIC APPROACHES IN THE CLINICAL AND PROGNOSTIC STRATIFICATION OF CHRONIC LYMPHOCYTIC LEUKEMIA
… such as long interspersed nuclear elements-1 (LINE-1), Alu and satellite α DNA (SAT-α DNA), reported to be associated with chromosomal instability. Our analysis was performed in a panel of 77 CLLs and 7 healthy donors using robust quantitative Pyrosequencing methodology to detect the …
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Genetic analysis of resistance to Myzus persicae (Sulzer) in Nicotiana tabacum L.
… Introductions 1462, 1118, 1112, 1024, breeding line 1-35 and cultivar NC 745. Inheritance studies showed that the resistance is a recessive trait controlled by three separate, non-linked loci; such that a homozygous recessive at any one locus will condition for the resistance reaction. Among the …
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Mechanisms of toxicity in cell models of FSHD
… intergenic loci that are enriched for Alu and LINE-1 elements, endogenous retroviruses, as well as from pericentric sequences enriched forhuman satellite II (HSATII) repeats. Similar to pericentromeric repeat expression in the early mouse embryo, bidirectional HSATII transcription appeared to …
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Can Addressing Language Skills for Fifth Grade ELLs in a Multiplication Curriculum Help Address the Achievement Gap in Math? A Multiplication Workbook for Big Kids
… equal groups, make and array, use a number line, 1 digit by 1 digit and 2 digit by 1 digit. Each lesson integrates an overview of a vocabulary word which is the concept of the lesson. Students will be able to practice their oral language development as they deepen their understanding of …
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On the distribution of central values of Hecke L-functions
… of the Riemann zeta function on the critical line 1/2 + <em>it</em> can be naturally interpreted as questions regarding the family of <em>L</em>-functions over Q associated to the archimedian characters<em> ψ (k)</em> =<em> k -it</em> at the center point 1/2. There are many families of …
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