Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 295 for “"LC-MS/MS"”.
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Studium nových syntetických drog metodou LC/MS/MS
… vybrán mefedron včetně jeho metabolitů metodou LC-MS/MS. Byl studován standard, negativní moč s přídavkem mefedronu a reálný vzorek moči potkana, kterému byl podán mefedron. Ve vzorku moči byl detekován mefedron a některé jeho metabolity. Získané informace o zkoumaných látkách mohou být následně …
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Innleiðing einnar mæliaðferðar á LC-MS/MS fyrir átján sterahormón
… liquid chromatography tandem mass spectrometry (LC- MS/MS). Today LC-MS/MS is considered a gold standard for steroid hormone measurements. LC- MS/MS technique is both quantitative and qualitative and able to quantitate very low concentrations of analytes and determine complex molecule structures. …
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Computational Analysis of LC-MS/MS Data for Metabolite Identification
Metabolomics aims at the detection and quantitation of metabolites within a biological system. As the most direct representation of phenotypic changes, metabolomics is an important component in system biology research. Recent development on high-resolution, high-accuracy mass spectrometers enables …
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Characterization of Glycans Derived from Biological Samples Using LC-MS/MS
… prevalent post-translational modifications (PTMs) is protein glycosylation, which enhances several biological functions, such as protein stability, localization, cellular communication, inflammation, immune responses, and host-pathogen interactions. Unusual protein glycosylation has been linked …
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Resíduos de glyphosate em grãos de café determinados por LC- MS/MS
… acoplada à espectrometria de massas tandem (LC-MS/MS) (Capítulo 1). Em sequência, esse método foi aplicado em um estudo a campo para avaliar a possível contaminação de grãos de café verde após a aplicação do glyphosate em diferentes estágios de desenvolvimento da cultura do café (Capítulo 2). …
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Computational Tools to Expand the Coverage of Metabolite Profiling via LC-MS/MS
… of the common metabolite profiling technologies, LC-MS/MS, and takes two steps to expand the coverage of current metabolite profiling experiments.</p><p>First, a method to enable collection of MS/MS data from a greater portion of the detected ions is presented. This is done by collecting …
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classCleaner: A Quantitative Method for Validating Peptide Identification in LC-MS/MS Workflows
… liquid chromatography-tandem mass spectrometry (LC-MS/MS) shotgun proteomics infers the peptide sequence of each measurement, there is inherent uncertainty in the identity of each peptide and its originating protein. Removing misidentified peptides can improve the accuracy and power of downstream …
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Entwicklung von Screeningverfahren für Arzneistoffe und Metaboliten mittels LC-MS und LC-MS/MS
Zunächst wurde ein LC-MS Screeningverfahren weiterentwickelt basierend auf einer bereits existierenden Screening Methode auf körperfremde Substanzen mit einer ESI-CID Massenspektrenbibliothek. Diese Bibliothek musste neu erstellt werden wegen Umstellung von der Software-Version eines Macintosh …
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A Data Attribution-Based Approach to Model Diagnosis in LC-MS/MS Structure Prediction
… liquid chromatography tandem mass spectrometry (LC-MS/MS) is a challenging task with far-reaching implications in many areas such as drug discovery, environmental science and metabolism research. Yet, despite its importance and significant efforts to develop machine learning (ML) models for the …
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DEVELOPMENT OF A LC/MS/MS ENZYME METHOD FOR N8 - ACETYLSPERMIDINE MEASUREMENTS IN ENZYME ASSAYS
… can be determined using either a Varian 1200L LC/MS/MS or an API 3000 LC-ES (+)/MS/MS. An acetylation-derivatization method was developed because N<sup>8</sup> -acetylspermidine and spermidine are hard to purify from a biological sample since they are not retained on a CIS solid phase …
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Extraction and analysis of pharmaceutical residues in environmental samples using SPE with LC-MS/MS
… and liquid chromatography mass spectrometry (LC-MS). Monolithic silica based stationary phases were used for the development of high performance liquid phase separations of common pharmaceuticals, the antifouling and anti-dandruff agent zinc pyrithione and a range of illicit drugs and abused …
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LC-MS/MS determination of various drugs of abuse and metabolites in municipal wastewater effluent samples
… liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the quantification and confirmation of 8 drugs of abuse (cocaine, codeine, MDMA, methadone, methamphetamine, morphine, nicotine, and oxycodone) and their various metabolites (acetylmorphine, cotinine, EDDP, amphetamine and …
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Applications of LC-MS/MS in forensic toxicology for the analysis of drugs and their metabolites
This thesis studied opioids and alcohol in forensic toxicology by LC-MS/MS, which avoids time-consuming procedures involving hydrolysis, extraction and derivatisation. Initially, a method was validated for quantification of opioids and unhydrolysed polar metabolites in autopsy specimens and was …
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Metabolomic Analysis of Bacterial Cell Wall Intermediates Using LC-MS/MS: Peptidoglycan Pathway Response to Different Antibiotics
… Part I of my dissertation describes a novel LC-MS/MS assay for the determination of in vivo levels of cytoplasmic PG-linked intermediates in S. aureus. Quantitative analysis of UDP-linked intermediates is challenging, in part due to the lack of commercial available intermediates for use as …
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Schnelles und zuverlässiges therapeutisches Drug-Monitoring mit HPLC-UV und LC/LC-MS/MS aus komplexen Matrizes
… einer zuverlässigen Detektion wurden HPLC- und LC-MS-Methoden erarbeitet. Die Bestimmung per Massenspektrometer bietet die Möglichkeit einer noch spezifischeren Detektion im Vergleich zu klassischen Techniken wie UV-VIS- und Fluoreszenz-Detektion. <br>Für Fluconazol wurde eine …
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Erhebung pharmakokinetischer Daten von Cisaprid, SC-72393, Haloperidol, Linezolid, Methotrexat und Ketoprofen nach Methodenentwicklung und Validierung durch LC-MS/MS-Detektion
… müssen. Aus diesem Grund ist der Umbau oder die Umstellung der Methode von einem zum anderen Wirkstoff ohne nennenswerten Zeitverlust ein maßgeblicher Faktor. Als Technik, die diese Anforderungen optimal erfüllt, hat sich in den letzten Jahren die LC-MS/MS-Technik etabliert. Sie ist den bislang …
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Διερεύνηση της παραγωγής αντιγονικών επιτόπων από την αμινοπεπτιδάση ERAP1 με βιβλιοθήκες πεπτιδίων και φασματοσκοπία μάζας συζευγμένη με υγρή χρωματογραφία (LC-MS/MS).
… του ΕΚΕΒΕ «Αλέξανδρος Φλέμινγκ», με τη χρήση timsTOF Ultra 2 συζευγμένο με το σύστημα nanoElute 2 UPLC. Από την ανάλυση επίσης ανιχνεύθηκαν περίπου 50.000 εννιαμερή, τα οποία στη συνέχεια τοποθετήθηκαν σε δύο ξεχωριστά μίγματα, όπου το ένα περιείχε την αμινοπεπτιδάση ERAP1, ενώ το δεύτερο δεν …
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Development of an LC-MS/MS method for the analysis of triacylglycerols from meat and application in the discrimination of cooked meat products
… high performance liquid chromatography (RP HPLC) separation of animal fat triacylglycerols (TAGs) has been developed for coupling with atmospheric pressure chemical ionization tandem mass spectrometry using an ion trap mass spectrometer. The method developed offers significant improvements on …
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The determination of β-endosulfan and endosulfan sulfate in human serum with dialkylphosphate metabolites as urinary markers using LC-MS/MS electrospray ionization
Two separate bioanalytical methods were developed, validated and applied to determine agricultural exposure to organochlorine and organophosphorus pesticides using different biological matrices as reference sources. The method that was validated for the quantification of the organochlorine …
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Determination of Rifapentine and 25-O-desacetyl Rifapentine from 100 µl human breastmilk by LC-MS/MS using protein precipitation and solid phase extraction
… method with detection by mass spectrometry (LC-MS/MS) is described for the quantification of rifapentine and its metabolite, 25-O-desacetyl rifapentine in human breastmilk, using rifampicin-d3 as an internal standard. An AB Sciex 4000 mass spectrometer at unit resolution in the multiple …
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