Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Knockout Model"”.
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THE MOLECULAR AND BEHAVIORAL CHARACTERIZATION OF THE MURINE CRE – LOXP SKELETAL MUSCLE SYNTHESIZED BDNF KNOCKOUT MODEL
… tissue was not significantly reduced in our knockouts, and was significantly elevated in the brain. Behavioral analysis of barbering failed to identify meaningful differences between BDNF<sup>lox+/+ </sup>mice and other transgenic lines when controlling for age and sex.</p>
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Investigation of Adiposity Phenotypes in AA Associated With GALNT10 & Related Pathway Genes
… quantitative trait loci (eQTL) data, and a mouse knockout model, in addition to furthering our knowledge of genes in the pathway in which GALNT10 acts. Previously, genome-wide association studies in African Americans (AA) have identified common variants upstream of GALNT10 locus for association …
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Electroproduction of the Phi(1020) Vector Meson at 4 GeV
… below cΔ𝜏 ≈ 1 fm. Within a simple optical model framework, the data show that 𝜙 meson has a smaller size than the 𝜌. The measured 𝜙 cross-section dependence on Q² is in a good agreement with previous measurements and well-described by the phenomenological Pomeron-exchange model. Our …
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Characterization of Cre Mouse Models to Target CNS Barriers for Generating Conditional Knockouts of ABC Transporters
… and have been studied using global transporter knockout mouse models. Since the CNS compartment and location of transporters is unique at each barrier site, a global knockout model does not address questions of how each barrier site contributes to ineffectiveness of drugs getting into the CNS. …
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Genetic modeling of the Hippo pathway in hematopoietic stem cells
… cells. In Article I we developed a new way of modeling genetic changes by combining genetic engineering of murine ES cells with blastocyst complementation. This approach avoids the cost and time constraints associated with the creation of standard transgenic mouse strains while taking advantage …
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WWC2 Is a Novel Regulator of GABAAR Expression, Synaptic Function, and Dendritic Morphology in Excitatory Hippocampal Neurons
… hippocampus. Using a forebrain-specific knockout model, I demonstrate that WWC2 negatively regulates surface GABAAR expression in the 1-month-old mouse hippocampus. Additionally, I demonstrate that the GABAAR recycling regulators HAP1 and GRIP1 are overexpressed in the membrane fraction …
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The role of 4-hydroxynonenal in cellular signaling mechanisms
… retinal pigment epithelial cells (RPE28 SV4) as models, in proposed studies we addressed the hypothesis that a physiological level of LPO product 4-hydroxynonenal (HNE) is necessary for maintaining key proliferation, adhesion, and survival signaling by regulating expression of genes involved in …
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Towards elucidating the function of the oocyte Subcortical Maternal Complex: A multi-omic analysis of an Nlrp5-null murine model
… SCMC proteins, demonstrating that the *Nlrp5* knockout model can effectively interrogate the function of the complex at large. GDF9 and BMP15, regulators of ovarian follicle development via paracrine signalling, were also notably reduced in *Nlrp5*-null oocytes. Other proteins altered in …
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Regulation of Excitatory Neurotransmission, Synaptic Plasticity, and Learning by Cyclin-Dependent Kinase 5
… several conditional Cyclin-dependent kinase 5 knockout model systems. Soon after conditional loss of Cyclin-dependent kinase 5, mice displayed improved hippocampal learning and enhanced synaptic plasticity in the hippocampal Schaffer collateral pathway. The genetically enhanced mice displayed …
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Role of ATRX in colorectal cancer metastasis
… retardation x-linked (ATRX) is a chromatin remodelling protein with established roles in regulating chromatin conformation, genomic stability, and homologous repair. ATRX is frequently mutated in many cancer types and in colorectal cancer (CRC) loss of ATRX is associated with the CRIS-B …
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Establishing the role of RNF4 in the vertebrate DNA damage response
… DNA damage response. <br/> <br/>A homozygous knockout of RNF4 in the DT40 chicken lymphocyte cell line was generated to investigate the involvement of vertebrate RNF4 in protecting cells against DNA damage. Although the complete loss of RNF4 did not affect cell proliferation or cell cycle …
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Metabolic control of immune cell fate by hypoxia-inducible factors
… is explored. Using a mouse T cell-specific FIH knockout model, FIH is shown to regulate T cell differentiation in an oxygen-dependent manner. Furthermore, by considering a metabolic network of related enzymes that compete for the same cosubstrates, FIH activity is predicted, and demonstrated, to …
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Investigating the T cell-specific role of Interleukin-4 receptor alpha (IL-4Rα) in tuberculosis (TB)
… in TB disease by making use of T cell specific knockout model, iLCKCreIL-4Rα-/lox. We found that absence of IL-4Rα on T cells results in a delay in the recruitment of T cells to the lung. This was demonstrated by decreased CD4 and CD8 T cell numbers during acute infection compared to littermate …
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Taming a Potent Mutator: Identification and Characterization of Novel Mechanisms of Regulating Antibody Diversification in B-Lymphocytes
… single ubiquitin moiety. Further, a conditional knockout model of RNF126 was generated and used to determine that RNF126 plays a role in vivo in fine-tuning AID activity during SHM and CSR. The findings presented here demonstrate the utility of a novel screening technique to search for …
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Impact of the chromatin remodeller SMARCAD1 on murine intestinal intraepithelial lymphocyte and white adipose tissue biology.
Impact of the chromatin remodeller SMARCAD1 on murine intestinal intraepithelial lymphocyte and white adipose tissue biology. Chromatin remodelling factors use the energy of ATP hydrolysis to drive the movement of and/or affect molecular changes to the nucleosome. One such factor, SMARCAD1 …
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Characterization of the Functional Role of the Intracellular Cholesterol Transporter StARD4 in Knockout Mice, and Investigation of Epigenetic Modulation of ApoA-I Transcription
… by SREBPs and we have chosen to make a mouse knockout model to characterize its role in-vivo. StARD4, expressed primarily in liver and macrophages, is a known intracellular cholesterol transporter previously shown to be down-regulated ~2 fold in liver, by high cholesterol feeding. It is …
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Unraveling the Interaction Between Beige Adipocytes and the Sympathetic Nervous System
… we used an adipocyte-specific Prdm16 knockout mouse model to ablate beige adipocyte function and demonstrated that the density of sympathetic parenchymal innervation depends on the presence of functional beige adipocytes. These results suggest that communication between beige …
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Modeling of Cns Cancer With A Focus On The Immune Component
… well as the patient setting which these studies model, the immune component of disease progression and treatment plays a strong role in each and is the primary focus of the works contained.</p> <p>Craniopharyngioma is a relatively rare tumor in adults. Although histologically benign, it can be …
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SITUATION MOUSE ALL FLOXED UP: EXPLORING PHENOTYPIC ABNORMALITIES IN BDNF FLOXED MICE
… communities. It is essential that our animal models accurately and predictably depict intended phenotypes, necessitating careful colony monitoring and maintenance. Volunteer animal staff in the Ottem Laboratory became concerned when mice with LoxP sites flanking the BDNF gene depicted …
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Identifying Genes and Novel Variants Involved in Nonsyndromic Hearing Impairment, and Assessment of the Psychosocial Burden of Hearing Impairment in Cameroon
… have demonstrated that mdx mice, (an animal knockout model for DMD), have an increased threshold for hearing when compared to wildtype mice. However, the contribution of DMD to HI in humans has not been extensively studied. Besides, most of the previous studies on DMD were conducted in …