Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"Human Embryo"”.
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Anne McLaren and the Human Embryo Research Debates in Britain, 1982–1990: Sociological Biography in the Analysis of Public Debate
… in the public and parliamentary debates on human fertilisation and embryology (HF&E) in Britain between 1982 and the resulting Act in 1990. A focus on McLaren’s contributions to these debates elucidates how the case in favour of embryo research was constructed. Drawing especially on the use …
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Medical biotechnology law in South Africa : a human rights analysis of selected topics
In this thesis I analyse the human rights dimensions of the South African law on four topical medical biotechnology subjects, namely human embryo research, the use of human gametes, autologous tem cell therapy, and private stem cell banking. In all four cases, my analyses give specific prominence …
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L'embryon ex utero : entre l'être et le néant
… and ethical issues. The possibility to create embryos in a laboratory and to allow them to survive outside the womb questions well-known legal ideas. The author analyses the legal status of ex utero human embryo and compares it to that of in utero fetus, considering all necessary distinctions …
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WNT signalling in the patterning of human gastruloids
The study of early human development poses a challenge due to technical and ethical limitations, particularly during the initial four weeks following fertilisation. During this critical period, the fundamental aspects of the body plan are established through a process known as gastrulation, which …
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Embryo Adoption: Implications of Personhood, Marriage, and Parenthood
… will influence his moral belief regarding embryo adoption. In Chapter One, I consider the personhood of the human embryo. If the human embryo is a person, we are morally obligated to permit the practice of embryo adoption as an ethical means to save human persons. However, for those who do …
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Methods for Dissecting High Dimensional Single Cell RNA Sequencing Data
… high resolution gene expression dynamics during human embryo pre-implantation blastocyst development. Through FFAVES, I expose rare cell types and cell type specific gene expression, by mitigating the contribution of technical confounders such as batch effects and false negative drop outs. To …
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Extracellular Signalling and Stem Cell Self-Renewal
In preimplantation mouse embryos, signalling and gene regulatory networks cooperate to determine lineage segregation, and modulating signalling in vitro allows for stem cell populations to be established from these lineages. Fibroblast growth factor (FGF) signalling triggers the differentiation of …
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Investigating the consequences of chromosome abnormalities arising during pre-implantation development of the mouse
The majority of human pre-implantation embryos created through in vitro fertilization (IVF) are mosaic as they are constituted of a mixture of diploid and aneuploid cells. Chromosome abnormalities are widely believed to contribute towards the relatively low success rates of IVF treatment. …
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The physico-chemical and biological analysis of microbial biosurfactants (BSs) for applications towards cancer therapies, wound healing dressings and drug delivery
… of 24, 48 and 72 h treatment on non-cancerous human embryo kidney (HEK 293), human Caucasian breast adenocarcinoma (MCF-7) and human leukaemia monocyte (THP-1) cell lines. The BSs were subsequently formulated in the form of niosomes initially in deionised water, which was followed by …
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Defining the essential regulators of naive human pluripotent stem cell reprogramming
Human pluripotent stem cells (PSCs) exist in two distinct states – naïve and primed, which are interconvertible. Naïve PSCs uniquely harbour several desirable properties that primed PSCs do not, including their ability to model human pre-implantation biology and developmentally-regulated epigenetic …
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Multiomic Atlasing of Human Mesenchyme Development
Development of human connective tissue, termed ‘mesenchyme’, is a dispersed process in utero involving a diversity of cell lineages across organ systems. Diseases of these cell lineages, including fibroblasts and cartilage, affect numerous organ systems throughout human lifespan, manifesting as …
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Demonstration of new subtypes of adenovirus 7 in South Africa, and probing oesophageal carcinoma cell lines for evidence of adenovirus or of other oncogenic viruses
… carried out in 2 parts: 1. Genome analysis of human adenovirus species 7; 2. Search for a possible viral aetiology in oesophageal carcinoma. Sixteen laboratory isolates of adenovirus species 7, isolated in South Africa between 1975 and 1986, were characterized by restriction endonuclease …
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Illuminating the Black Box: Defining and Modeling Peri-Implantation Human Embryogenesis
Human pre-gastrulation development is a complex process where the single cell zygote transitions to the multilineage blastocyst, comprised of the epiblast (precursor of the fetus), hypoblast (precursor of the yolk sac) and trophoblast (precursor of the placenta). The blastocyst then implants into …
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The Role of Tyrosine Phosphorylation In The Functions of The Tumor Suppressor Gprc5A
… its expression is suppressed in the majority of human non-small cell lung cancer cell lines and tissues. This differential expression has led to the idea that GPRC5A is a potential tumor suppressor. This notion was supported by the finding that mice with a deletion of the Gprc5a gene develop …
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Trophoblast specification and differentiation potential in early human and non-human primate embryogenesis
… by trophoblast, the outer layer of the early embryo. Trophoblast is an extraembryonic tissue that gives rise to the placenta, which sustains fetal growth and development. Human embryo implantation and early trophoblast development have remained elusive due to the inaccessibility of early …
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Pluripotency and patterning in nonhuman primate embryogenesis
Pluripotent cells of the embryo have the remarkable ability to form all somatic tissues and the germ line. Mice have long provided a framework for our understanding of this property of the early embryo. However, primate embryogenesis differs in anatomical architecture, developmental timing, and …
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Single-cell genomics in rabbit and mouse elucidate eutherian embryonic development
… the use of model organisms to gain insight into human health and development. Traditionally, the mouse has been the favoured vertebrate model, due to its experimental and genetic tractability. Non-rodent embryological studies however highlight that many aspects of early mouse development, …
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Optimizing embryo culture conditions and spent culture media analysis as predictors of embryo quality and pregnancy
… evaluate various culture conditions to improve embryo development, and secondly, to analyze spent culture media for any biomarkers that may be predictive of embryo health. Single-step and sequential culture media were compared in both Planer and EmbryoScope™ incubators. Single-step media …
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Self-Renewal Requirements of Human Embryonic Stem Cells and Their Engraftment Potential in Mouse Blastocysts
<p>Human embryonic stem cells (hESCs) are a unique population of cells derived from a 6 day old human embryo that can be maintained indefinitely in vitro and have the ability to differentiate to all adult cell types. In addition to their potential for cell based therapies in the treatment of …
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