Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 76 for “"HUVEC"”.
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MICRO-CT EVALUATION OF MSC/HUVEC/HSC-MEDIATED ANGIOGENESIS IN LARGE PCL-ßTCP CONSTRUCTS IMPLANTED IN NUDE MICE
… cord derived vascular endothelial cells (HUVEC) or hematopoietic stem cells(HSCs)-mediated formation of microvascular networks in a biocompatible PCL-ßTCP hybrid scaffold in an immuno-compromised nude mouse. The PCL-ßTCP scaffold (30% PCL: 70% ßTCP; 70% porosity) with interconnected open …
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Modulatory effect of EVOO polyphenols and their metabolites on inflammatory response in Adenocarcinoma Colon Cells (Caco-2) and Human Umbilical Vein EC (HUVEC)
… Caco-2 cell monolayer) and endothelial level (in HUVEC cell monolayer), using pro-inflammatory stimuli, such as LPS and/or a hyperglycemia (HG) condition. Their modulatory action was evaluated focusing on the alteration of tight junctions (TJ) and the activation of cellular pathways, as …
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Regulation of VEGF-activated signalling by the plasma membrane calcium ATPase 4 in endothelial cells
… RNA isolated from PMCA4-silenced (or control) HUVEC. Changes in gene expression after PMCA4 knockdown have been further validated by TaqMan-based qPCR in HUVEC or HDMEC. RNA levels of PMCA4 in aging HUVEC were analysed by TaqMan qPCR using RNA isolated from HUVEC cultured from different …
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Interaktion von Listeria monocytogenes mit Endothelzellen
… makrovaskuläre Nabelschnurendothelzellen (HUVEC) verwendet. Die für die Ausbildung der Blut-Hirn-Schranke verantwortlichen mikrovaskulären Hirnendothelzellen (BMEC) unterscheiden sich aber deutlich von den makrovaskulären HUVEC. In der vorliegenden Arbeit wurde die Interaktion von L. …
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MUTAGENIC EFFECTS OF CHROMIUM (IV) ON HUMAN ENDOTHELIAL AND LUNG EPITHELIAL CELLS
… on human umbilical vein endothelial cells (HUVEC) and human bronchial epithelial cells (BEAS-2B) were investigated through morphological, cell viability, gene expression, and cDNA sequencing assays. The cell morphology showed both HUVEC and BEAS-2B cells suffered significant but different …
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Remodelado de la entrada de calcio en la angiogénesis: papel de las proteínas ORAI1 Y SARAF
… de CE humanas derivadas de la vena umbilical (HUVEC); y afecta significativamente al desarrollo de los vasos de la retina de ratón neonato. Encontramos que Orai1 y SARAF colocalizan en HUVEC y están implicados en el incremento de la [Ca2+]i mediada por VEGF. En esta misma línea, encontramos que …
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Einfluß von Steroidhormonen auf die Endothelzellproliferation und die Expression von Wachstumsfaktoren und ihren Rezeptoren
… und ihren Rezeptoren zu untersuchen. <br>HUVEC, HUAEC sowie HDMVEC und Fibroblasten wurden bis zur Konfluenz kultiviert. Die Endothelzellen wurden mit FACS-Analysen identifiziert. Durch immunzytochemische Untersuchungen wurde die Existenz von Östrogen- und Progesteronrezeptoren gesichert. …
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Μελέτη της αντι-αγγειογενετικής δράσης των συνθετικών πεπτιδίων HB-19 και N6L
… και την in vitro προσκόλληση των κυττάρων HUVEC. Τα πεπτίδια αυτά παρουσιάζουν in vivo ογκοκατασταλτική δράση και βρέθηκε να αναστέλλουν την αγγειογένεση στο σύστημα της χοριοαλλαντοϊδικής μεμβράνης εμβρύου όρνιθας. Επίσης αναστέλλουν τη μετανάστευση των κυττάρων HUVEC στο in vitro σύστημα …
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Identification of Anti-Beta2 Glycoprotein I Auto-antibody Regulated Gene Targets in the Primary Antiphospholipid Syndrome Using Gene Microarray Analysis
… in human umbilical vein endothelial cells (HUVEC) in response to affinity purified anti-2GPI. Sera were collected from patients with PAPS and IgG was purified using HiTrap Protein G Sepharose columns. Polyclonal anti-2GPI were prepared by passing patient IgG through NHS activated sepharose …
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Defining the human endothelial transcriptome
… cultured human umbilical vein endothelial cells (HUVEC), we have found approximately 54% of all genes to be expressed at a detectable level in HUVEC under basal conditions. The set of highest expressed genes is enriched in nucleic acid binding proteins, cytoskeletal proteins and isomerases as well …
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Effect of ATF2 transcription factor on DLL4 gene expression in angiogenesis
… ATF2 functionality was suppressed by infecting HUVEC with an adenovirus expressing a phosphorylation-mutant, dominant-negative version of ATF2 (Ad-ATF2AA). HUVEC infection with an adenovirus encoding GFP (Ad-GFP) was used as a control. Alternatively, ATF2 expression in HUVEC was suppressed by …
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Interaction of Human umbilical vein endothelial cells and human hepatocytes celline on Gelatin-VEGF Microspheres and PHBV-HGF Microspheres in co-culture
… co-culture through semi-permeable membranes of HUVEC cells on Gelatin-VEGF microspheres and Hep3B cells on PHBV-HGF microspheres was done. Cells grown by direct random co-culture and co-culture through the membranes exhibited higher viability and high P-450 cythocrome activity throughout the …
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Fatty acid regulation of adhesion molecule expression in human vascular cells.
… in human umbilical vein endothelial cells (HUVEC), smooth muscle cells (SMC) and THP-1 monocytes. The expression of the adhesion molecules was assessed by fluorescence activated cell sorting (FACS) on cell treated with n-3 PUFA and CLA for 24 hours followed by 6 hours cytokine stimulation. …
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Schaffung dreidimensionaler Kapillarnetze in extrazellulären Kollagenmatrices aus genmodifizierten humanen Endothelzellen.
… getestet. Humane Endothelzellen, sogenannte HUVEC, wurden aus Nabelschnüren isoliert und in speziellem Nährmedium kultiviert. Proliferationsassays in zweidimensionaler und dreidimensionaler Zellkultur mit verschiedenen Konzentrationen von rekombinanten Wachstumsfaktoren bFGF, VEGF und EGF …
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Mechanisms responsible for homocysteine mediated damage to human endothelial cells : the role of oxidative stress in atherogenesis.
… effects. Human umbilical vein endothelial cells (HUVEC) were cultured and treated chronically with DL-Hcy for 5-9 days. An in vitro flow system was also used to characterize the different types of interactions between DL-Hcy-treated HUVEC and neutrophils under physiological flow conditions. EC …
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Endotheliale Superoxidanionen–Bildung
… - - Bildung leistet. Weitere Untersuchungen in HUVEC sollten zeigen, ob und wie sich sowohl De – als auch Hyperpolarisation der Zellmembran auf die O2 - - Bildung auswirken, welches Enzym hierbei eine Rolle spielt und welche Signaltransduktionsmechanismen beteiligt sind. Zur O2 - - Messung an …
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Development and Characterisation of a Vessel-On-a-Chip Model of Inflammation and Inflammatory Haemostasis
… of human umbilical vein endothelial cells (HUVEC) against a 3D extracellular matrix (ECM). Stimulation with TNF-α induced HUVEC inflammatory cytokine expression and promoted neutrophil transmigration towards a chemoattractant gradient. Differences in neutrophil transmigration were observed …
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The Roles of the Collagen Binding Integrins in the Regulation of Endothelial Cell Function
… using human umbilical vein endothelial cells (HUVECs). First, quantitative real-time polymerase chain reaction (qPCR) was performed to quantify the expression levels of the four integrins. Functional roles of these integrins were then investigated using commercially available inhibitors …
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Antithrombotic agents under flow conditions
… with human umbilical vein endothelial cells (HUVEC) or extracellular matrix (ECM) has been developed. A method to investigate thrombin generation (TG) in platelet rich defibrinated plasma was validated and used to investigate inhibition by two distinct classes of antithrombotic agents: …
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PMCA as a regulator of calcium/calmodulin-dependent signal transduction pathways
… and the plasma membrane calcium ATPase (PMCA) in HUVEC primary endothelial cells. PMCA2 has been identified as the major isoform interacting with eNOS in endothelial cells. The interaction between the two proteins has been mapped to the region 735-934 of eNOS and 462-684 of human PMCA2b. NO …
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