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Showing 1 to 20 of 95 for “"HRP"”.
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Characteristics and applications of the enhanced HRP-catalyzed oxidation of luminol
… years is the enhanced horseradish peroxidase (HRP)-catalyzed luminol reaction. Generally, the enhancers are halogenated phenols or aromatic amines. Unfortunately, only limited work has been done to characterize the action of these enhancers. The work reported to date has been empirical …
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Studies on the internalization and intracellular transport of horseradish peroxidase in Chinese hamster ovary cells
Soluble horseradish peroxidase (HRP) is internalized by Chinese hamster ovary cells, a cell line of fibroblastic origin (Adams et al., 1982). We have confirmed this result by showing no inhibition of uptake in the presence of divalent cation chelators (EGTA Mg or EDTA), excess (19 mg/ml) yeast …
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Human resource planning practices in the Omani Public Sector: An exploratory study in the Ministry of Education in the Sultanate of Oman
Human resource planning (HRP) is the management process that helps organisations prepare for the number of employees and the types of skills needed to achieve organisational goals and objectives. In short, the aim of HRP is to have the right people in the right place at the right time. However, …
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The Contribution of Human Resources Planning in Public Institution’s Performance: A Case Study of Mlele District Council.
… that will lead to effective implementation of HRP at MDC and examine the outcome of Human Resources Planning in public institution towards its performance. Mlele District council was used as a case study. Sample selection involved a simple random sampling was 50 employees were selected as a …
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Evaluation of immobilizing horseradish peroxidase and alcohol oxidase in PVA-AWP polymer
… and stability of horseradish peroxidase (HRP) and alcohol oxidase (AOX) when immobilized in a photo-crosslinkable and water-soluble polymer, poly (vinyl alcohol) azide-unit water pendant (PVA-AWP). The immobilized enzyme films were to be used in biosensing applications and their stability …
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Engineered heme peroxidases as genetically encoded probes with diverse applications in cell biology
… been developed for EM. Horseradish peroxidase (HRP), a multifunctional reporter enzyme widely used in bioanalytical assays, has been utilized for EM because of its ability to generate electron-dense deposits. In addition, HRP produces extremely sensitive chemiluminescent, colorimetric, and …
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Treatment of 2,4-dichlorophenol using soybean peroxidase.
… research has focused on horseradish peroxidase (HRP) as the enzyme source. Recently, researchers have discovered that SBP is thermally more stable and can work in a different pH range than HRP. Since the soybean seedcoat is a waste product of the soybean industry, developing a value-added process …
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The Free Radical Nature of Compound I From Horseradish Peroxidase and Chloroperoxidase
… Is were prepared from Horseradish Peroxidase (HRP), Chloroperoxidase (CPO), mesohemin substituted Horseradish Peroxidase (M-HRP) and deuterohemin substituted Horseradish Peroxidase. The Compound Is were analyzed by visible absorption, electron paramagnetic resonance (EPR), Mossbauer and …
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Meerrettich Peroxidase : Modifikationen und Anwendungen in Biosensoren
… Peroxidase“ (engl. horseradish peroxidase, HRP) verwendet. Solche HRP-Elektroden werden für die Messung von Wasserstoffperoxid (H2O2) eingesetzt. H2O2 wird im Körper von weißen Blutkörperchen produziert, um Bakterien abzutöten, wird teilweise ausgeatmet und kann in kondensierter Atemluft …
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THERMOSTABLE EXOSHELLS AS TOOLS FOR RECOMBINANT PROTEIN EXPRESSION AND IN VIVO DELIVERY
… to GFPuv, horseradish peroxidase (HRP), and Renilla luciferase, and Photinus pyralis luciferase, improving yields of functional in vitro folding by approximately 100 fold. Encapsulated enzymes retain the ability to metabolize small molecule substrates, presumably via four 4.5-nm …
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The kinetics of endosome processing
… in comparison with the behaviour of endocytosed HRP (horse-radish peroxidase) as a fluid-phase contents marker. The pooling and/or segregation in the endosome was measured by determining co-localization with HRP. Colocalization of the two markers in the endosome is studied by using the ability of …
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Engineering a highly enantioselective horseradish peroxidase by directed evolution
… useful enzyme, horseradish peroxidase (HRP), toward a chiral phenol. In doing so, we discovered that mutations close to the active site not only preserve HRP catalytic activity but impact its enantioselectivity far greater than distal mutations. We thus examined how a single mutation …
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Inhibition and inactivation of prostaglandin synthase-2 and horseradish peroxidase
… archetypical peroxidase horseradish peroxidase (HRP) revealed the importance of hydrogen bonds to the distal catalytic residues, in particular the distal arginine of HRP. The ability of peroxidases, including PGHS-2, to oxidize a wide variety of compounds makes the design of selective, reversible …
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Machine Learning Asset Allocation
… optimización llamado Hierarchical Risk Parity (HRP). El enfoque de HRP utiliza Machine Learning y teoría de grafos para construir un portafolio diversificado basado en la información contenida en la matriz de covarianza de los activos. El HRP se construye mediante la metodología de clustering …
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Determination of hydrogen peroxide using immobilized chemiluminescence reagents and flow injection analysis
… (rsd) = 2.5%). Next, horseradish peroxidase (HRP) was immobilized on nylon membranes. Luminol was immobilized on to an anion exchange resin and packed in a column. When used in the FIA mode, stoichiometric amounts of luminol could be released from the column by injecting another anion with …
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A systematic study of the effect of ultrasound on food enzymes and bioactives in model and real food systems (strawberry puree)
… activity of commercial horseradish peroxidase (HRP), PPO in mushroom extract, and both enzymes in strawberry puree. Structural alterations of these enzymes after US treatment were studied using time resolved fluorescence. The impact of US treatment on the antioxidant activity, polyphenols, and …
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Assessment Of The Effectiveness Of Human Resources Planning in Achieving Organizational Objectives: A Case of Tanzania Public Service College and Immigration Department
… for Social Sciences). The findings show that HRP improves organizational performance and it is very effective in enabling an organization to achieve its objectives. HRP and succession planning are nowpracticed in some organizations and they help to improve performance and stability of …
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Medical Constraints in Spaceflight: Venturing Beyond Low Earth Orbit
… Medical Capability (ExMC) portion of the HRP integrated research plan presented in NASA HRP-47065 Rev B.
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Application of additives in horseradish peroxidase-catalyzed removal of phenol derivatives from aqueous solution.
… can be used in the horseradish peroxidase (HRP) catalyzed polymerization of phenolic compounds to increase the enzyme turnovers by more than 100-fold. In addition to polyethylene glycol (PEG) and gelatin, some polyelectrolytes may also prove effective as additives. The HRP saving is …
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Analysis of the peroxidase-catalysed oxidation of hydroxamic acids
… H-bond network involving Pro139, His42, Arg38 in HRP and His109, Gln105, Asp108, Arg255 and Glu258 in LPO as the hydroxamic acid group of all substrates investigated was bound in the vicinity of these active site residues. However, ambiguity remains as to the precise mechanism of proton transfer. …
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