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Showing 1 to 20 of 22 for “"HPTLC"”.
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Quantitative HPTLC
… the High Performance Thin Layer Chromatography (HPTLC) technique. HPTLC allows quantitative as well as qualitative results of much smaller amounts. in some cases down to the picogram level. With these advancements, the limiting factor in detection of smaller concentrations has become the plate …
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Characterisation of sandalwood essential oils using high performance thin-layer chromatography and high resolution gas chromatography mass spectrometry
… High Performance Thin-Layer Chromatography (HPTLC) has yet to be fully explored as an alternative to GC-MS in authenticating sandalwood essential oils of different species. In this study, sandalwood essential oils were characterised using HPTLC and high resolution GC-MS. The potential of …
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Thermal, oxidative and hydrolytic stability of selected frying shortenings evaluated by new and conventional methods
… by high-performance thin-layer chromatography (HPTLC). Under static heating conditions, varying heating periods or shortening types had significant (P<0.000l) effects on the resulting data of the following tests: free fatty acids; polar component; total volatiles; dielectric constant; viscosity; …
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Utilization of a scientifically operated charge-coupled device detector for high-performance thin-layer chromatographic analysis
A high-performance thin-layer chromatographic (HPTLC) separation system coupled with a scientifically operated charge-couple device detector (CCD) has been developed to improve the identification and quantitative analysis of pharmaceutical products and natural toxins. The combination of the fast, …
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Flight activity and hemolymph diacylglyceride concentrations in Heliothis zea (Boddie) (Lepidoptera:Noctuidae)
… performance thin-layer chromatography method (HPTLC) was developed to detect lipids in microgram (ug) quantities of hemolymph. The effect of age, diurnal rhythms, and flight activity on hemolymph 1,2-diacylglyceride concentrations in male and female Heliothis zea (Boddie) (Lepidoptera: …
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Influence of flight activity and octopamine on hemolymph trehalose titers in Heliothis zea (Boddie) (Lepidoptera: Noctuidae)
… by high performance thin-layer chromatography (HPTLC) at various ages and times of the day and related to flight activity. Effects of octopamine injection or stress on trehalose levels were also quantified. Flight activity was measured with a 32-channel computerized actograph that simulated …
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Mitochondrial Function as a Tool for Assessing Function, Quality and Adulteration in Medicinal Herbal Teas
… to OLTE assays with significant results. HPTLC was used for comparison to existing industry methods. Results 2000 μg / mL OLTE induced a 14.88% (p < 0.05) rise in reactive oxygen species (ROS) in MCF7 cells after 3 hours followed by a 14.73% (p < 0.05) drop at 72 hours. Oxygen consumption …
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Analysis of two single trait loci affecting flavonol glycoside accumulations in Arabidopsis thaliana natural variations
… high performance thin layer chromatography (HPTLC) was used to rapidly screen large amounts of plant samples. HPTLC was also employed to purify flavonoid derivatives that were then used for the identification of sugar groups by gas chromatography mass spectrometry (GC-MS). Twelve different …
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Isolierung und strukturelle Charakterisierung von E-Selektin-bindenden Gangliosiden aus humanen Granulozyten
… zur Detektion von Zellbindungen an HPTLC-getrennten Gangliosiden. Die Lokalisation gebundener Zellen erfolgte mit Antikörpern und unter Zuhilfenahme eines Viabilitätstestes. Für die Zelladhäsionsmethoden wurde ein Anwendungsspektrum erarbeitet, wobei die Leistungs- und …
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Synthese eines Polymerskeletts in Liposomen über pH-abhängige Membranadsorption von Monomeren
… Liposomen wurde durch Monomeranalytik mittels HPTLC bestimmt. Die anderen verwendeten Monomere erfüllten z.T. nicht die erwarteten Ergebnisse (u.a. zu schnelle Permeation von verkapseltem Monomer aus Liposomen, Probleme bei der Liposomenpräparation). <br>Um die innere Struktur des Polymers …
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Einschluss von 5-Fluorouracil (5-FU) in vesikuläre Phospholipidgele : in vitro und in vivo Untersuchungen zur Stabilität, Pharmakokinetik und antitumoralen Wirksamkeit
… in vitro Freisetzung, Lipidstabilität (HPTLC) und Phasenübergangstemperatur (DSC). <br>In vivo wurden zwei Formulierungen an tumortragenden athymischen Nacktmäusen in insgesamt sieben Wirksamkeits- und Verträglichkeitsstudien mit vier Tumormodellen auf ihre antitumorale Wirksamkeit …
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Carbohydrates in Glycine max (L.) Merr. fruits during early ontogeny
… by high performance thin-layer chromatography (HPTLC). Sucrose was present as a lesser component. Amylase activity was maximal at anthesis, but declined with increasing fruit size. Beta-amylase comprised a high percentage of the amylolytic activity in the developing fruits. Embryo development in …
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FOOD AND FEED SAFETY IN THE CIRCULAR ECONOMY
… and identified main phenolic compounds by HPTLC; and Chapter 4 produced a proposal for a harmonized feed-consumption database built on a standardized feed classification, including a model data structure and two applied case studies (genetically modified feed and contaminants). Results show …
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Untersuchungen zur funktionellen Bedeutung von Gangliosiden bei der E-Selektin-vermittelten Zell-Zell-Adhäsion
… und eine humane Hirngangliosidfraktion mittels HPTLC getrennt und im Overlay-Assay auf Anwesenheit von CSLEX- und E-Selektin-Epitopen getestet. Anhand von statischen und dynamischen Zell-Zell-Adhäsionstests wurde die funktionelle Bedeutung von Gangliosiden bei der E-Selektin-vermittelten …
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Cryptosporidium Parvum Microbial Adhesion: Interactions Between Sporozoites and Host Cells
… by semipreparative HPLC followed by preparative HPTLC. Biochemical analyses, TLC staining techniques, mass spectrometry, and elemental analysis were used to partially characterize this lipid. The results of these analyses indicate the isolation of a small molecular weight, nonsaponifiable lipid …
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The Effect of Alhagi maurorum Medik on Mitochondrial Function
… effects. Characterisation of the extract through HPTLC and LC-MS analysis showed presence of more than one compound in the extract.
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Thin layer chromatography-matrix assisted laser desorption ionisation-mass spectrometry of pharmaceutical compounds.
… device and by use of special Si 60 F[254] HPTLC-MALDI targets. Using both approaches sensitivities in the high fmol range were obtained. To minimise matrix interference, which can suppress analyte signals, the application of suspensions of particles of different materials and sizes (Co-UFP, …
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Methoden zur Charakterisierung von großtechnisch hergestellten Lipoplexen zum Gentransfer
… des DOPE-Anteils im Lipid, mit einer HPTLC-Methode konnten sowohl der DOPE-, als auch der Cholesterol-Anteil bestimmt werden. Die Bestimmung des DNA-Anteils wurde ebenfalls mit spektroskopischen Methoden durchgeführt. Die Lyophilisate wurden durch Bestimmung des Restfeuchtegehaltes und …
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The anti-cancer activity of extracts derived from millets and Kraalbos
… extraction were subjected to fractionation using HPTLC. Results from these extracts show that only the methanol fractions of the Babala and Enforcer millets exhibited short-term cytotoxic activity against the MCF-7 and the MDAMB- 231 breast cancer cell lines. Five Kraalbos extracts (KB1, KB2, KB3, …
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Fatty acid synthase is a major polypeptide constituent of cytosolic lipoprotein and is associated with components of the milk lipid secretory pathway
… but not from involuting mammary glands. HPTLC analysis of lipids extracted from the low density FAS (LDFAS) complex demonstrated the presence of the five major milk phospholipids as well as triacylglycerols, cholesterol, unesterified fatty acids, and diacylglycerols. ³²P-labeled …
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