Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 172 for “"HEK293"”.
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Using high-throughput proteomics to identify novel engineering targets for enhanced rAAV production in HEK293 cells
… of the necessary genetic components into HEK293 cells. Unfortunately, low yields during this process prevent rAAV production from meeting increasing demands. Optimisation of rAAV manufacturing through rational design will require an increased understanding of the HEK293 cell production …
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Towards the establishment of a highly effective adeno-associated virus production by leveraging next generation sequencing and transcriptomics in HEK293 cells
… processes in human embryonic kidney cells (HEK293) by establishing a deeper understanding of the underlying mechanism through a holistic approach. To this end, several approaches were combined, such as the comparison of two HEK293 cell lines with different productivity profiles and their …
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GPR55 and N-acyl Amino Acids
… cell lines were utilised, a stably transfected HEK293 cell line that overexpresses 3xHA N-terminus tagged hGPR55 (hGPR55-HEK293 cells) and control HEK293 cells. In addition, the DU145 a prostate cancer cell line which is reported to endogenously express GPR55 was investigated. N-acyl amino acid …
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Investigation of selected autophagic proteins and the effect of tPS1-GFP expression.
… with a truncated presenillin 1 construct, in HEK293 cell line showed structural changes in the cell in the early stages of autophagy. However, it seems that the autophagic process had failed to clear the presenilin-1 aggregates as would be expected in the normal state and appeared to lead to …
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Studies of CD36 interacting with fatty acids, oxidized low-density lipoprotein, and the cellular plasma membrane
… into the molecular mechanisms of uptake, HEK293 cells expressing CD36 were studied by cell biological and fluorescence methods. To test our hypothesis that the PM is not an impermeable barrier to FA and that FA move into cells by diffusion via their uncharged form, we first applied …
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Proteomic analysis of TRIF interactome and functional characterisation of novel TRIF interacting proteins
… Overexpression of ADAM15 in HEKT293, HEK293-TLR3 and HEK293-TLR4 inhibited TRIF, TLR3 and TLR4-dependent activation of NF-κB, IFNβ and Rantes promoter activation, respectively. Moreover, downregulation of ADAM15 by esiRNA enhanced poly(I:C) and LPS-induced cytokine/chemokine secretion …
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Profiling the activity of GPR55 antagonists against recombinant and endogenous GPR55
… on LPI-mediated GPR55 responses, in a HEK293 cell line stably expressing GPR55 (hGPR55-HEK293) and also in a prostate cancer cell line that expresses GPR55 endogenously at high levels (DU145). Antagonist effectiveness was also examined in a neuronal model of Alzheimer’s disease (AD). …
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GPR50, a potential factor involved in psychiatric disorders interacts with Alzheimer’s disease-related protein β- secretase (BACE1)
… increased endogenous BACE1 activity in HEK293 cells, so I hypothesised that there is a functional interaction between the two. In this thesis, I investigated the relationship between GPR50 and BACE1 by identifying the effects of GPR50 on BACE1 expression and function, which may provide …
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Study of Presynaptic Calcium Channels and a Novel Calcium Channel Calhm1
… we have used overexpression of Calhm1 gene in HEK293 cells as well as in dissociated neuronal cultures. Our experiments show that Calhm1 modulates calcium signaling in HEK293 cell and in certain neurons. Calhm1 overexpression also modulates spontaneous synaptic transmission.
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Endogenous Stress Signaling within Human Multicellular Aggregates (Spheroids)
… monolayers. Herein it is shown that HEK293 spheroid arrest and recovery requires the co-activation of both NF-kB and JNK, and chemical inhibition of either NF-κB nuclear translocation or JNK phosphorylation leads to cell death. Cytokine profiling within the aggregates during the …
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An In Vitro Assessment of The Potential for Engineered Nanoparticles to Modulate Innate Immunity
… in vitro, two Human Embryonic Kidney cell lines (HEK293) were employed; one stably expressing TLR 2 (HEK293-TLR2) and the other devoid of TLRs (HEK293-WT). A select group of ENPs; silver nanoparticles (75 nm), gold nanorods (48 nm + 4 nm), and carboxylated, and pristine semi-conducting (0.8 nm) …
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Caracterización de la función de la proteína humana znf330/noa36 mediante estudios interactómicos y de mutagénesis con el sistema crispr/cas9
… de las proteínas recombinantes en células HeLa y HEK293. En este análisis se detectó también una posible interacción con la proteína Enhancer of Rudimentary Homolog (ERH) que en células humanas está involucrada en la regulación del ciclo celular. Dado que estos resultados indicaban que NOA36 …
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Characterization of Molecular Content, Cytotoxicity, and Anti-Inflammatory Activity of Extractions from Eupatorium serotinum
… The cytotoxicity of the different extracts on HEK293 cells was determined by a cell death assay and a cell viability assay. HPLC was performed on the extracts and compared with a Helenalin standard to determine the possible presence of sesquiterpene lactones isolated from <em>E. serotinum.</em> …
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The Role of 5-Alpha Reductase 3 In Steroid Metabolism
… <p><strong>Materials and Methods</strong></p> <p>HEK293 cells were transfected with the SRD5A3 human cDNA ORF Clone from<strong> </strong>OriGene. Steroid substrates (testosterone, progesterone, androstenedione, epitestosterone, 11-ketotestosterone, 11α-hydroxytestosterone, …
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Degradational and Transcriptional Investigations of the RAF Kinase Inhibitory Protein
… by ubiquitin-proteosomal pathway in either human HEK293 cells or rat AR42J cells. I found that the RKIP promoter reporter was not significantly repressed by Snail in transfected HEK293 cells but was subject to repression by the GSK-3 inhibitor BIO compound in both E-box (putative Snail binding …
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Degradational and Transcriptional Investigations of the RAF Kinase Inhibitory Protein
… by ubiquitin-proteosomal pathway in either human HEK293 cells or rat AR42J cells. I found that the RKIP promoter reporter was not significantly repressed by Snail in transfected HEK293 cells but was subject to repression by the GSK-3 inhibitor BIO compound in both E-box (putative Snail binding …
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TCF4-mediated Transcriptional Regulation of Krüppel-like Factor 4 Gene
… 중재되는지 알아보기 위해, Western blot 실험을 통해 HCT116 세포와 HEK293 세포에서 TCF4 발현을 관찰하였다. TCF4 단백질이 HCT116 세포에서는 발현 되었지만 HEK293 세포에서는 나타나지 않았다. 인간 KLF4 promoter에 존재하는 두 개의 conserved 지역에 TCF4 단백질이 binding 하는지 알아보기 위해, electrophoretic mobility shift assay를 수행하였다. ATCAAAG 지역에는 TCF4 binding complex band가 관찰되었지만 …
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HECAT5 regulates cell proliferation via interaction with M3R in cell type-specific manner
… low but HECAT5 enhanced its level. Meanwhile, in HEK293 cells, basically its level was high but HECAT5 decreased it. Besides, F3 and HEK293 cells showed different signaling pathways, ERK and AKT. Collectively, our study uncovers that a novel HECAT5 gene may play a role in regulating the stemness …
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REGULATION OF GLYCOSYLATION IN MAMMALIAN CELL LINES: INSIGHTS FROM STRUCTURAL GLYCOMICS, ANALYSIS OF GLYCOSYLATION-RELATED GENES AND FUNCTIONAL RNAI SCREENS
… mammalian cell lines, namely CHO-K1, BHK-21, HEK293, COS-7 and 3T3, were characterized by a combination of mass spectrometric and chromatographic techniques. Such glycomic profiling revealed several immunogenic non-human N-glycan structures in BHK-21 and 3T3 cells. The cell lines were also …
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Binding and Signaling Differences between Prostaglandin E1 and E2 Mediated by Prostaglandin E Subtype Receptors
… The S211L and R214L mutants expressed in HEK293 cells lost binding to [3H]PGE2. This study found that the non-conserved S211 and R214 of the hEP3 are involved in PGE2 recognition. The mutant S211L was able to give a calcium signal with PGE1, but not with PGE2. This implied that the …
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