Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 94 for “"Genome Stability"”.
-
Nuclear Encoded Proteins Important in Mitochondrial Genome Stability
The mitochondrion is widely known to be the site of cellular respiration and the factory of cellular energy. Similar to the nucleus, mitochondria house genetic material (mtDNA), which is responsible for the production of proteins essential to mechanisms required for cellular respiration. …
-
RNA EXPORT FACTOR ELF1 MAINTAINS GENOME STABILITY IN FISSION YEAST
Genome instability, or frequent mutations within a genome, is deleterious and a hallmark of carcinogenesis. Here, I identified that Elf1, a AAA+ family ATPase involved in mRNA nuclear export, prevents genome instability. Upon deletion, elf1Δ cells grow slowly, forming small colonies. Curiously, …
-
Functional Roles of Nucleases in DNA Metabolism and Genome Stability
… on two distinct areas that are important for genome stability. Both areas focus on DNA repair pathways that require the action of nucleases, specifically Exonuclease 5 and Ribonuclease H2. First, I describe the biochemical and molecular characterization of the novel Exonuclease 5 family of …
-
Functional investigation of the role of the retinoblastoma protein in genome stability
Genome instability is an enabling characteristic of cancerous cells. It has recently been discovered that the retinoblastoma protein (pRB), typically known for its role in cell cycle regulation, also aids in the maintenance of genome stability. Intriguingly, mutations to the pRB gene, RB1, can …
-
ZSCAN4 AND REPLICATION STRESS: SAFEGUARDING GENOME STABILITY IN MOUSE EMBRYONIC STEM CELLS
… of DNA replication is essential for maintaining genome stability. However, replication can be disrupted by both endogenous and exogenous factors, leading to a condition known as replication stress (RS). RS slows down and eventually stalls replication forks. If stalled forks are not resolved, they …
-
Investigating the role of protein ubiquitylation in the maintenance of genome stability
… (ICLs). The importance of HR for maintaining genome stability is underscored by the observation that loss-of-function mutations in HR factors are commonly seen in human cancers. In this thesis, I report the use of a key hallmark of HR-deficient cells, namely synthetic lethality with the …
-
Micropropagation of Begonia and a study of genome stability in Begonia rex
… colchicine treatments to destabilize the B. rex genome have also been described. Variants produced from these treatments indicate the utility of in vitro procedures for the expression of induced somatic variation. Colour variants produced from irradiation treatment have been cultured and prove …
-
DDI1/2 and RTF2-Dependent Regulation of RNASE H2 at the Replisome Ensures Genome Stability
… DNA, inefficient replication fork restart, genome instability, and cell death. During unperturbed replication, RTF2 travels with the replication fork (Kottemann et al., 2018). Here we examine the function of RTF2 and address why RTF2's removal from the replisome is necessary for recovery …
-
Characterizing the contribution of the LXCXE binding cleft to pRB-mediated genome stability and tumor suppression
… and if compromised, can lead to genomic instability. Genomic instability is known to be an active contributor to tumorigenesis, rather than being a by-product of malignant progression. The retinoblastoma protein (pRB) is the prototypic tumor suppressor. Its tumor suppressive properties are …
-
Tumor Suppressor function of the deubiquitinating enzyme BAP1 and its substrate gamma-tubulin In regulation of cell cycle and genome stability
… of the chromosomes and chromosomal instability, which are commonly observed in cancer. There are a number of surveillance mechanisms to ensure errors are corrected, and if not, the cells commit suicide (apoptosis). In cancer, this tightly regulated process malfunctions as a result of …
-
Novel proapoptotic p63 isoforms are driven by an endogenous retrovirus in the male germ line of humans and great apes, likely increasing genome stability
TAp63, ein Homolog des Tumorsuppressors p53, hat die Fähigkeit, die weibliche Keimbahn zu beschützen, indem es Oozyten mit schweren DNA-Schäden nach Bestrahlung eliminiert. Obwohl die Expression von TAp63 in männlichen Keimbahnzellen vermutet wird, ist noch unklar, ob TAp63 auch als Wächter der …
-
Investigating the mitotic roles of Topoisomerase 2 Alpha and Topoisomerase 2 Beta-Binding Protein 1 in the maintenance of chromosome structure and genome stability
… scaffold, recruiting factors to the genome during transcription, replication, and the DNA damage response. Recently, a role for TOPBP1 in M phase has also emerged. In the first part of this thesis, I focus on the role of TOP2 in orderly metaphase congression and the maintenance of …
-
Expansion microscopy to visualize cell-cycle events in the abbreviated cell cycle in <i>Caenorhabditis elegans</i> (roundworm) germline stem cells
… understood form of the cell cycle essential for genome stability in stem cells. To visualize cell-cycle events in the abbreviated cell cycle, we optimized a protocol for expansion microscopy in stem cells of the reproductive system of the model organism C. elegans. Optimizations included …
-
Regulation of Pumilio RNA Binding Proteins by Long Noncoding RNA NORAD
The mammalian genome is extensively transcribed and encodes thousands of long noncoding RNAs (lncRNAs). Defining the mechanism of action of lncRNAs has been a critical challenge and priority for understanding their biological functions. An important example of this is the lncRNA NORAD, which is a …
-
The Role of Esc2 and DNA in Smc5/6-mediated Sgs1-Top3-Rmi1 Sumoylation
… of cells and requires precise copying of the genome, which is challenged by many obstacles or damages such as DNA double-strand breaks (DSBs). There are several mechanisms to deal with DSBs and replication blockages, including homologous recombination (HR). HR leads to the formation of DNA …
Page 1 of 5