Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 104 for “"Gene sequencing"”.
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Improving Medicare & Medicaid Reimbursement Framework for Molecular Diagnostics Tests Using Gene Sequencing: A Case Study in Health Economics
… the cost estimates does not change the general results. This model, which can objectively compare two therapy regimes in terms of economic benefits derived by stakeholders, can be applied to evaluate the economic value of competing treatment regimes.</p>
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BIOINFORMATIC PIPELINES AND MICROBIAL DATABASES FOR THE ANALYSIS OF DAIRY COWS 16S RRNA-GENE SEQUENCING DATA: A BENCHMARKING STUDY.
… diversi database - 16S-ITGDB, RPD, SILVA e Greengenes -, di cui due in due differenti aggiornamenti (SILVA versione 132 e versione 138, Greengenes versione 13.9 e versione 2). Le prestazioni di queste combinazioni saranno testate utilizzando due comunità fittizie e quattro set di dati relativi …
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Comparison of Subterranean Termite (Rhinotermitidae: Reticulitermes) Gut Bacterial Diversity Within and Between Colonies and to Other Termite Species Using Molecular Techniques (ARDRA and 16S rRNA Gene Sequencing)
… rDNA restriction analysis (ARDRA) and 16S rRNA gene sequencing. It was determined that ARDRA was an effective technique for characterizing the diversity of the termite gut microbiota. Of the 512 clones analyzed in the ARDRA study, 261 different ARDRA profiles were found. Forty-two 16S rRNA gene …
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Prevalence of the related treponematoses syphilis and yaws and co-occurrence of pathogens causing similar skin lesions in Ghana
… diseases, two Treponema pallidum subspecies, are genetically similar, but they have always been studied independently. In this context, this study aimed to assess the prevalence of these treponematoses and determine the co-occurrence of pathogens causing similar skin lesions in the Ashanti region …
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Design, Cloning, and Expression of Rat Preptin and Alanine Analogs
<p>Alanine scanning mutagenesis was employed to determine the structural epitopes of preptin; a newly discovered polypeptide found on the proinsulin-like growth factor II. The alanine scanning mutagenesis was accomplished by the use of, cloning, affinity chromatography, gel electrophoresis, gene …
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Strain identification, viability and probiotics properties of lactobacillus Casei
… casei group by carbohydrate fermentation, PCR, gene sequencing and pulsed field gel electrophoresis, 2) to investigate tolerance of Lactobacillus strains in the presence of acid, bile salts and the or bile salt deconjugation, 3) to investigate viability of freeze dried Lactobacillus strains at …
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American Alligator Fecal Coliform and Pathogen Identification, Genotypic Fingerprinting, and REP-PCR Bacterial Source Tracking in South Carolina
… the API 20E biochemical test system and 16S rRNA gene sequencing methods, in the identification of alligator cloacal isolates. The second component evaluated the ability of Repetitive Extragenic Palindromic PCR (REP-PCR) to produce identifiable, unique, and stable genomic fingerprints from …
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Microbiology of the sulfur-dominated glacial ecosystem at Borup Fiord Pass, and high Arctic low-temperature spring associated microbial mats, The
… for microbial community composition (16S rRNA gene sequencing), function (metagenomic sequencing), and aqueous geochemistry. 16S rRNA gene sequencing data revealed Flavobacterium was common to almost all samples. Sulfur-oxidizing microorganisms (Sulfurimonas, Sulfurovum, and Sulfuricurvum), and …
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Enumeration, isolation, identification and probiotic characterisation of lactic acid bacteria from Nigerian human breast milk
… PCR (rep-PCR). A total of nineteen groups were generated from the rep-PCR DNA profiles. This was followed by genotypic identification using 16S rRNA gene sequencing. The 16S rRNA gene sequencing identified LAB and non-LAB. Most of the LAB isolates belonged to the Lactobacillus genus, the rest …
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Matryoshka genetics: identification of disease-associated mutations in the human genome
… chain reaction (ARMS-PCR), Sanger-based full gene sequencing, multi-gene panel next-generation sequencing, and deletion/duplication analysis by array comparative genomic hybridisation (aCGH). The assays are variously applied to single gene and chromosomal disorders, and in some instances …
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DEEP LEARNING ALGORITHM FOR MOLECULAR CLASSIFICATION OF ENDOMETRIAL CANCER FROM WHOLE SLIDE HISTOPATHOLOGY IMAGES
… through IHC, POLEmut identification requires gene sequencing, which is costly and often unavailable. In this study, we developed a fully supervised deep learning (DL) model to classify EC molecular subtypes directly from H&E-stained whole-slide images (WSIs). From an initial cohort of 1,362 …
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Characterisation of vaginal Prevotella strains from a cohort of South African adolescent girls and young women with and without bacterial women with and without bacterial vaginosis
… all isolates. A subset was speciated by 16S rRNA gene sequencing to be P. bivia (n=36) and a putative P. melaninogenica (n=1) and were carried forward for further phenotypic characterisation, including antimicrobial resistance testing by Etest methodology, and quantification of biofilm formation …
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The effect of tylosin exposure or exclusion on liver abscess prevalence, fecal pathogen populations, and the microflora of finished beef products from feedlot cattle
… and finished beef products through 16S rRNA gene sequencing and shotgun sequencing from feedlot cattle with and without exposure to tylosin. Overall, liver abscess rates were lower among cattle exposed to tylosin. However, there were no differences among treatment groups for any of the sample …
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Diet Induced Alteration of the Murine Intestinal Microbiome After Antibiotic Ablation
… by Gram stain, quantitative flow cytometry, next generation sequencing of the V3 or V4 regions of the 16S rRNA gene, fluorescence microscopy, and sequence analysis of the tuf gene, which can distinguish among Streptococcus and Staphylococcus genera.;Results: Fecal samples from antibiotic-treated …
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ANALYSIS OF MICROBIOME IN VARIOUS HUMAN MEDICAL CONDITIONS USING NEXT-GENERATION SEQUENCING OF THE 16S rRNA GENE
… 43 healthy and diseased patients using 16S rRNA gene sequencing. We identified a rich microbial landscape, identifying over 338 bacterial taxa. Notably, <em>Acinetobacter</em>, <em>Cutibacterium</em>, <em>Acidovorax</em> and <em>Herbaspirillum</em> were prevalent in the ocular samples and<em> …
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The Phylogenetic Analysis Of Genes Encoding Specific Steps In The Glutathione Pathway Within Industrial Beer Brewing Strains: Saccharomyces Cerevisiae, Saccharomyces Pastorinus, And The Spoilage Microbe Brettanomyces
… the GSH pathway: GSH1, GSH2, GLR1, and SOD1 genes, to trace the evolutionary history of beer strains are lacking. As a result, the investigator sought to elucidate the phylogenetic relationships between four commonly used industrial beer strains: California Ale, London Ale, Oktoberfest, and …
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Symbionts and immune defence in lepidopteran crop pests
… pest species are constantly being dispersed and generate great concern due their resistance against chemical pesticides and Bt crops. These factors emphasize the importance to the development of technologies for rapid field diagnosis and specific environmentally-safe pest control. Here we show …
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Molecular characterization of mycoplasmas species isolated from the genital tract of Dorper sheep in South Africa
… from this disease using conventional and genetic molecular methods. In this study, 16SrRNA gene-based PCR assays and gene sequencing was used for the detection and characterization of Mycoplasma species from cases of ulcerative vulvovaginitis and balanoposthitis in Dorper sheep in South …
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MUTAGENIC EFFECTS OF CHROMIUM (IV) ON HUMAN ENDOTHELIAL AND LUNG EPITHELIAL CELLS
… through morphological, cell viability, gene expression, and cDNA sequencing assays. The cell morphology showed both HUVEC and BEAS-2B cells suffered significant but different degrees of damage and demonstrated features indicating carcinogenesis when these cell lines were exposed to …
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Improving Our Comprehension of Microbial Communities
… microbial communities. The use of universal genes, such as those for small subunit ribosomal ribonucleic acid (SSU rRNA), or randomly sequenced genome fragments form the foundation upon which a majority of microbiologists evaluate community structure, metabolic capabilities and community …
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