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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 240 for “"GST"”.
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A non-well-founded set theory (GST)
Open Restriction set for Item 19544 on 2020-11-02T17:15:11Z with date null by astein@illinois.edu.
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Cytochrome c peroxidase : its role in nitrosative stress and purification as a GST-fusion protein
… of this DNA into the pGEX 2T vector yields the GST-CCP construct, and expression of this construct in E. coli produces ~530 mg GST-CCP fusion protein per 12 L of culture. Purification of the fusion protein involves a single affinity chromatography step and free CCP is obtained by cleavage of the …
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Expression und Aufreinigung von intrazellulären Anteilen des Interleukin-4-Rezeptors als GST-Fusionsproteine und Messung von Protein-Protein-Wechselwirkungen
… intrazelluläre Anteile des IL-4 Rezeptors als GST-Fusionsproteine exprimiert. GST-E1, in dem das cytoplasmatische membranproximale 1/3 von IL-4Ralpha (173 AS) einschließlich des Box 1 Motivs an GST fusioniert ist, konnte nach differenzierter De- und Renaturierung und Bindung an …
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Mechanisms of cell cycle remodeling at the MBT during the development of Xenopus laevis embryos
… were treated with two cell cycle inhibitors, GST-D34Xic1 and XChk1. Injection of embryos with GST-D34Xic1, a stoichiometric inhibitor of cyclin E/Cdk2, delays degradation of cyclin E and onset of the MBT. GST-D34Xic1 lowers Wee1 level, a kinase that maintains Cdks in an inactivate state. …
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Nanometer-scale temperature measurements of phase change memory and carbon nanomaterials
… temperature rise of thin film Ge2Sb2Te5 (GST) based PCM due to Joule, thermoelectric, interface, and grain structure effects. PCM has potential to reduce energy consumption and improve performance of future electronic memory. A new nanometer-scale thermometry technique is developed for …
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Biochemical and molecular basis for metabolism-based atrazine resistance in Amaranthus tuberculatus
… result of increased glutathione S-transferase (GST) activity in atrazine-tolerant crops such as maize and grain sorghum (Sorghum bicolor), or in atrazine-tolerant weeds such as Panicum spp. In previous research, two atrazine-resistant populations of tall waterhemp (Amaranthus tuberculatus) from …
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Value-added tax:Analysis of the supply of cross border electronic services
… offered at better prices (price excludes VAT/GST). This creates an unfair advantage for foreign suppliers over local suppliers. The problem with selling a product to a consumer over the internet is that no physical product crosses through any physical border post. These products are not …
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Development of antibodies for characterizing the Arabidopsis flavonoid biosynthetic pathway
… vector as fusions with glutathione Stransferase (GST) using standard directional cloning techniques. Analysis of crude extracts of Escherichia coli containing GST .. CHS or GST .. CHI fusion protein indicated that the cells expressed equivalent amounts per volume of culture. CHS and CHI were …
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Untersuchungen zur Substratspezifität der ADP-Ribosyltransferasen Clostridium botulinum C2-Toxin und Clostridium perfringens Iota-Toxin
… von Iota a und C2I kloniert, in E. coli als GST-Fusionsproteine exprimiert und hinsichtlich ihrer ADP-Ribosyltransferase-Aktivität und Substratspezifität in vitro geprüft. Zum selben Zweck wurden auch chimärische Proteine hergestellt, die aus verschiedenen Anteilen beider …
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Identification and Characterization of A Zebrafish Glutathione S-Transferase Pi
Glutathione S-transferases (GSTs) are a major group of Phase II enzymes involved in the detoxification of certain endogenous compounds such as reactive oxygen species (ROS) generated during metabolism, as well as in the detoxification of xenobiotics including drugs. In recent years, the zebrafish …
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Novel Cancer Therapeutics, the Generation of ROS, and Cell Survival
… unknown. The present studies examine whether the GST fusion protein, GST-MDA-7, and the adenovirus, Ad.mda-7, altered the growth and survival of the A498 and UOK121N RCC lines or radiosensitized GBM, respectively. Due to previous findings that the RCC lines, but not primary renal epithelial cells, …
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Differential tolerance of corn hybrids to metolachlor and its regulation by the safener benoxacor
… in the expression of glutathione S-transferase (GST) activity during early seedling development of 'Northrup-King 9283' corn may be of additional significance in its limited tolerance to metolachlor. The safener benoxacor was effective in protecting 'Northrup-King 9283' and other susceptible corn …
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The Mechanism of Chemoprotection by 1-Cyano-2-Hydroxy-3-Butene, a Natural Component of Cruciferous Vegetables
… a monofunctional DNA-directed increase in GST subunit Ya$\sb2,$ Yc$\sb2$ and QR mRNA levels, without a concomitant increase in mRNA levels of CYP 1A. This suggests that, in the previous study, the protection afforded against aflatoxin-induced carcinogenesis was through a monofunctional …
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The relationship between p53 mutations and differential sensitivity to anti-cancer drugs in oesophageal cancer cell lines
… (P-gp) and glutathione-S-transferase (GST), and p53 mutations were investigated. Chemosensitivity of the WHCO1, WHC03 and HCu-13 cell lines to 7 anti-cancer agents was determined. WHC03 was relatively resistant to cisplatin and transplatin, but sensitive to vinblastine, vincristine, …
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Studies of tumor promoters and drug-metabolic enzymes in hamster buccal pouch mucosa
… (GGT) and placentalglutathione S-transferase (GST-P) have been found to be increased during HBPM carcinogenesis. Whether such increases are oncofetal remains to be answered. There are few studies on the normal distribution of GGT and GST-P in hamster tissues. One experiment was designed to …
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Factors influencing arbovirus transmission: vector competence and the effects of virus infection on repellent response, oxidative stress, and glutathione-S-transferase activity
… oxidative stress and glutathione-S-transferase (GST) activity in Aedes albopictus. Virus infection had no effect on oxidative stress, but GST activity was significantly different for mosquitoes 3-days post-exposure. We found that oxidative stress levels and GST activity had an inverse …
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Trichuris Muris Whey Acidic Protein Induces Type 2 Protective Immunity Against Whipworm
… WAP (fragment 8) expressed as an <em>Na-</em>GST-1 fusion protein (r<em>Tm</em>-WAP-F8+<em>Na</em>-GST-1), generate a strong T helper type 2 (TH2) immune response when delivered as subcutaneous immunization formulated with Montanide ISA 720. Oral challenge with <em>T. muris</em> infective eggs …
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Developing a framework for assessing equity in contributions to the global goal on adaptation as part of the global stocktake
The global stocktake (GST) aims to assess collective progress in mitigation, adaptation, and the means of implementation and support as outlined in Article 14 of the Paris Agreement. The stocktake will be undertaken considering equity and the best available science. As a result, the GST requires a …
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Uniaxial optical phase change metamaterials
… can be achieved. In this paper, Ge₂Sb₂Te₅ (GST) is chosen as a model phase change material due to its high reflectance contrast between states, fast switching speeds, and high metastability. Here, we theoretically investigate the reflectance and form birefringence of GST-based optical …
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