Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"GEMMs"”.
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Fibroblast Heterogeneity In Pancreatic Cancer Immunity
… to assess genetically engineered mouse models (GEMMs) in which we could deplete aSMA<sup>+</sup> and FAP<sup>+</sup> CAFs. We found the aSMA<sup>+</sup> and FAP<sup>+</sup> CAFs were distinct subsets with tumor restraining and tumor supporting roles, respectively. aSMA<sup>+</sup> CAFs were …
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Developing Novel Models and Therapeutics for ZFTA-RELA Ependymoma
… the biology and treatment of cancer, no GEMMs of ependymoma have been generated: current models require invasive intracranial injection of cells or gene constructs during embryogenesis. This thesis describes research to generate and characterise novel GEMMs of ZFTA-RELA ependymoma as well …
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A Study of The Snd1/Prmt5 Axis In Liver Cancer By Genetic Mouse Models
… HCC using genetically engineered mouse models (GEMMs), though, it remains unknown if loss of SND1 or its methyl reading ability can protect against HCC formation. PRMT5 has been reported as upregulated in many cancers and may predispose hepatocytes to develop HCC. However, it remains to be …
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Assessing the temporal role of miR-200 loss in murine models of NSCLC
… In this study, we found that in the lungs of GEMMs harboring loss of miR-200 tumors, there was a shift in the immune microenvironment favoring pro-tumor activity. We saw these changes ahead of detectable epithelial-mesenchymal transition in the primary lesions, as IHC showed highly epithelial …
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An organoid platform to study alveolar stem cells in lung generation and cancer
… other hand, genetically engineered mouse models (GEMMs) recapitulate key aspects of human LADC including initiation from normal pulmonary epithelial cells and progression into a malignant state. The development of organoid technology has revolutionized the way we model cancer and a vast number of …
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Genetic analysis of Kmt2d loss in tumorigenesis using genetically engineered mouse models
… we utilized genetically engineered mouse models (GEMMs) to study the impact of <em>Kmt2d</em> loss during melanoma, lung, and urothelial carcinoma development.</p> <p> In a mutant <em>Braf</em>-driven melanoma, <em>Kmt2d</em> loss accelerated tumor development and caused tumor de-dedifferentiation …
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CRISPR-mediated interrogation of small cell lung cancer
… validated. Genetically engineered mouse models (GEMMs) of SCLC have been developed that recapitulate many key features of human SCLC. These models have been used extensively to investigate various aspects of SCLC biology, including tumor initiation, progression and metastasis. The development of …
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A broadened HLA ligandome uncovers new immunotherapy targets for pancreatic cancer + A prime editor mouse to model a broad spectrum of somatic mutations
… encoded in the mouse germline, prime editor GEMMs can mediate rapid and precise engineering of most cancer mutations, including many that are challenging or infeasible to achieve with other CRISPR technology. We demonstrate the utility of this system by mediating secondary Kras mutations and …
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Functional contribution of epithelial to mesenchymal transition program on KRASG12D targeting efficacy in pancreatic cancer
… we employed genetically engineered mouse models (GEMMs) of PDAC with deletion of the EMT transcription factor (TF) <em>Zeb1</em> (Zeb1<sup>cKO</sup>) and orthotopic models with conditional deletion of the EMT TFs <em>Snail</em> and <em>Twist</em> (ST<sup>cKO</sup>) to evaluate the role of EMT …
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Constructing and deconstructing cancer using CRISPR-Cas9
… existing genetically engineered mouse models (GEMMs) have led to critical insights into the initiation and progression of human cancer, their use for rapid functional characterization of new cancer genes has been historically limited, partly due to the cost and time required to generate …
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Cd47+ Exosomes Facilitate Effective Targeting of Oncogenic Kras In Pancreatic Cancer
… models, genetically engineered mouse models (GEMMs), as well as in a patient derived xenograft (PDX) model of pancreatic cancer, and significantly increased their overall survival. In addition we demonstrate that the enhanced efficacy iExosomes is in part due to enhanced survival of exosomes …
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Unique Transcriptional Profiles Underlie Osteosarcomagenesis Driven By Different P53 Mutants
<p>Missense mutations in the DNA binding domain of the <em>Trp53</em> gene are characterized as structural (<em>p53R172H</em>) or contact (<em>p53R245W</em>) mutations based on their effect on the conformation of the protein. These mutations show gain-of-function activities such as increased …
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Differential progression of pancreatic premalignancy in geographically distant murine transgenic cohorts uncovers key role of microbial metabolites
… this end, genetically engineered mouse models (GEMMs) have been developed with the mutations most commonly seen in the patient population. Expression of these mutations in select cellular compartments has resulted in the ability to recapitulate characteristics of human pancreatic cancer such as …