Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 30 for “"Expressed sequence tags"”.
-
Molecular Mapping of Disease-Related Expressed Sequence Tags and Resistance Gene Analogues in Soybean
… develop PCR-based markers from disease-related expressed sequence tags (ESTs) and resistance gene analogues (RGAs), 2) assess the utility of such markers by diversity analysis of 12 soybean parental lines, and 3) search for possible association of the markers with known disease resistance genes …
-
Identification and quantification of lipid metabolites in cotton fibers: Reconciliation with metabolic pathway predictions from DNA databases.
… into phosphatidic acid. A profile of expressed sequence tags (ESTs) for genes involved in lipid metabolism in cotton fibers was also obtained. This EST profile along with our lipid metabolite data was used to predict lipid metabolic pathways in cotton fiber cells.
-
Tissue-specific classification of alternatively spliced human exons
… were analyzed for tissue-specific skipping. Expressed sequence tags (ESTs) were aligned to exons and their tissue of origin and histology were identified. ACEs were also identified as a subset of the skipped exons. About 18% of the exons were identified as tissue-specifically skipped in one …
-
Gene discovery and expression profiling in the toxin-producing marine diatom, Pseudo-nitzschia multiseries (Hasle) Hasle
… and initiate characterization of actively expressed genes that control cell growth and physiology in P. multiseries, with the specific goal of identifying genes that may play a significant role in toxin production. The first step in gene discovery was to establish a complementary DNA (cDNA) …
-
Gene expression and biochemistry of isoprenoid biosynthesis in the glandular secretory trichomes of Artemisia annua
… Bead Beater based method. Expression patterns of expressed sequence tags from a trichome library whose Basic Local Alignment Search Tool (BLAST) results suggested a possible function in terpenoid metabolism were investigated by RT-PCR. Known terpenoid biosynthetic enzymes, such as …
-
Towards Cloning the Leaf Rust Resistance Gene Rph5
… the sub-centimorgan region around Rph5 using sequence-tagged site (STS) markers that were developed based on barley expressed sequence tags (ESTs) syntenic to the phage (P1)-derived artificial chromosome (PAC) clones comprising distal region of the rice chromosome 1S. Five rice PAC clones were …
-
Comparative genomics of early animal evolution
… using novel genomic data and publicly available expressed sequence tags (ESTs). I then move forward (Chapter 3) to use network-based methods to study the early evolution of the GPCR superfamily in Eukaryotes and animals. Finally (Chapter 4), I focus on the study of a specific subset of GPCRs (the …
-
Expressionsanalyse des humanen Histonsubtyps H1x
… die eine erhöhte Trefferrate von H1x-ESTs (expressed sequence tags) in Tumordatenbanken neuroendokriner Tumore (NETs) ergeben hatte, untersuchte ich die Expression von H1x in NETs aus Lunge und Gastrointestinaltrakt. Immunhistochemie, Western Blot-Analyse und real-time RT-PCR zeigen eine …
-
Plant-parasitic nematodes: from genomics to functional analysis of parasitism genes
… nematode Radopholus similis by generating expressed sequence tags (ESTs). The most striking discovery was tags corresponding to aWolbachia-like endosymbiont, which was subsequently located in the ovaria of R. similis. Numerous tags corresponding to parasitism genes with potential roles in, …
-
Soybean Resistance and Defense Responses Against Aphis Glycines
… on soybean genetic linkage group M by simple sequence repeat (SSR) molecular markers. Rag1 was flanked by Satt435 and Satt463 4.0 cM and 8.0 cM from Rag1, respectively, in the integrated map from Dowling x Loda and Dowling x Williams 82 F2:3 populations, and Rag(Jackson) was flanked by Satt435 …
-
Analysis of the Allergenic Potential of the Ubiquitous Airborne Fungus Alternaria Using Bioinformatics
… the analysis of A. alternata spore germination expressed sequence tags (ESTs), 2) the survey of global allergen homologues in fungal genomes, and 3) the first microarray experiment investigating airway epithelial cell responses to this fungus. In the first project, the analyses of the EST …
-
GeneSieve: A Probe Selection Strategy for cDNA Microarrays
… Often, cDNA libraries representing expressed genes of an organism are available, along with expressed sequence tags (ESTs). ESTs are widely used as the probes for microarrays. Designing custom microarrays, rich in genes relevant to the experimental objectives, requires selection of …
-
Gene discovery and expression profilng in the toxin-producing marine diatom, Pseudo-nitzschia multiseries (Hasle) Hasle
… and initiate characterization of actively expressed genes that control cell growth and physiology in P. multiseries, with the specific goal of identifying genes that may play a significant role in toxin production. The first step in gene discovery was to establish a complementary DNA (cDNA) …
-
Identification and characterization of protein phosphatase 2A mutants in Arabidopsis thaliana
… insertion was determined from DNA sequencing, expressed sequence tags and cDNA data. The presence of a full-length mRNA was not detected from five of the 13 mutant alleles (cl-1, c3-1, c3-3, c4-1 and c4-2) as determined by the reverse transcriptase-polymerase chain reaction. Wild type and …
-
The evolution of freeze tolerance in a historically tropical snail
… western Atlantic and Gulf of Mexico. I have used sequence data from one mitochondrial (CO1) and two nuclear markers (histone H3 and a mitochondrial carrier protein, MCP) to identify three cryptic species within Melampus bidentatus, and to infer their relationships to other Melampus. To identify …
-
Exploring the evolution of social behaviour using genomic data
… changes in the protein coding regions of brain expressed genes. To this end, we used 454 GS FLX sequencing technology to generate the brain Expressed Sequence Tags (ESTs) of twelve socially diverse bees. The ESTs were assembled into species-specific non-redundant contigs and singletons, which …
-
Positionsklonierung des Morbus Best-Gens VMD2
… Sequenzen, Kartierung von EST-Klonen ('expressed sequence tags'), RT-PCR-Analysen und, wenn erforderlich, 5'-RACE-Experimenten, acht neue Gene des Menschen zu isolieren. Von den charakterisierten Genen erwiesen sich mehrere als potentielle Kandidaten für VMD2. Ein Gen, provisorisch als …
-
An evolutionary genomics approach towards analysis of genes implicated in transmission of trypanosomes between tsetse fly and mammalian host
… genomes of several trypanosome species have been sequenced. Sequencing of additional neglected trypanosome species is in progress. The tsetse fly genome is currently being sequenced as part of the genomic activities of the International Glossina genome initiative (IGGI). In an attempt to support …
-
Caracterização de seqüências expressas do genoma café potencialmente relacionadas com a resistência a doenças
… do PBGC, foram identificadas ESTs (Expressed Sequence Tags) relacionadas a cada uma destas palavras. Outra estratégia utilizada foi a busca por similaridades entre algumas seqüências públicas envolvidas com a resistência do cafeeiro a doenças com as seqüências do PBGC, por meio do …
-
Systematic analysis of genes expressed in the retinal pigment epithelium (RPE) and identification of candidates for genetic susceptibility to age-related macular degeneration (AMD)
… analyse genes which are differentially expressed in the RPE, and to assess their possible association with mechanisms and pathways likely to be related to retinal disease, in particular AMD. Towards this goal, 2379 expressed sequence tags (ESTs) were established from an inhouse generated …
Page 1 of 2