Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 255 for “"Eukaryotic cells"”.
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"Effects of nonthermal plasma on prokaryotic and eukaryotic cells"
… Plasma-mediated germicide in prokaryotic cells and stimulatory and proliferative effects in eukaryotic cells were determined to be dose-dependent phenomena.
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Multiplex genome editing and gene regulation in eukaryotic cells
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2019-05-01
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Evaluating convolutional neural networks and transformer architectures for image-based prediction of protein localization in eukaryotic cells
… of protein subcellular localization in eukaryotic cells, using large-scale immunofluorescence image datasets. Methods: In this study, we comparatively evaluated convolutional neural network (CNN) architectures (DenseNet121, Xception, and InceptionV3) and transformer-based models (Vision …
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Keep the ORCs at bay : how eukaryotic cells ensure one round of DNA replication per cell cycle
During each cell cycle, eukaryotic cells must faithfully replicate their genome, ensuring exactly one full copy is made. Both under-replicating or over-replicating the genome can have deleterious consequences including cell death, genome instability and cancer. Thus, this process is tightly …
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Mitotic homologous recombination at engineered repeats in S. cerevisiae and in novel transgenic mice
… genetic and environmental factors that render cells susceptible to homologous recombination. Our goal is to elucidate the mechanisms of DNA damage-induced recombination and to determine the role of DNA repair enzymes in modulating homologous recombination in eukaryotic cells. Alkylating agents …
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Bioenergetics and metabolism of eukaryotic cell proliferation
… biomass accumulation and proliferation in eukaryotic cells. We first determined that the underlying mechanism behind why rapidly proliferating cells preferentially ferment the terminal glycolytic product pyruvate is due to an intrinsic deficiency of respiration to regenerate electron …
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Metakaryotic biology : novel genomic organization in human stem-like cells of fetal-juvenile development and carcinogenesis
… and genomic segregation that occur in mitotic eukaryotic cells. In order to discover how a genome apparently devoid of chromosomes might be organized, this thesis focused on recognizable DNA sequences common to all chromosomes: centromeres and telomeres. Fluorescence In Situ Hybridization …
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Biophysical studies of chromatin folding and unfolding
… a physical basis of coordinated DNA functions in eukaryotic cells. Chromatin folding is started with a linear long string of nucleosomes, connected by segments of DNA called linker DNA. Further folding forms the higher order structure, 30-nm fibers in which form most DNA in interphase nuclei is …
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Screens for non-eukaryotic initiation factor two alpha dependent activation of ATF4 translation
Cells must be able to respond to their environment. Inability to respond correctly to changes has direct consequences on cellular fitness. Eukaryotic cells have developed many different pathways to signal nutrient availability, cellular stress, and general environmental changes in order to maintain …
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Expression of single chain antibody-encoding genes in fission yeast and tobacco
… genes encoding single chain antibodies (SCA) in eukaryotic cells. During the course of this research, a new PCR-based strategy was developed that significantly reduced the time required for SCA gene synthesis. Although initially developed for the synthesis of SCA genes from templates of known …
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The Systems Biology of the Protein Quality Control
… main powerhouse for protein degradation in all eukaryotic cells. Proteostasis is maintained by the ubiquitin-proteasome system (UPS) targeting aberrant proteins whose accumulation and aggregation are hallmarks of aging and neurodegenerative disorders. Previous studies demonstrated complex …
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DNA polymerase beta inhibitor pamoic acid : toxicity to metakaryotic human cancer stem cells (HT-29)
Amitotic cells with large, hollow bell-shaped nuclei, or metakaryotic stem cells, are the post-embryonic stem cells of the fetal organs from about the fourth week post conception through physical maturity. These metakaryotic stem cells, after acquiring necessary genetic, and possibly other events, …
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An expanded search for RNA-programmable genomic engineering effectors
… of precise genome engineering in prokaryotic and eukaryotic cells.
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Localization and Function of Bacterial Type III and IV Effector Proteins
Eukaryotic cell signal transduction networks are highly dynamic and complex systems largely composed of signaling enzymes with modular protein interaction domains and subcellular localization motifs. These sophisticated regulatory mechanisms are crucial to the fidelity and efficacy of information …
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The role of the environmental stress response in aneuploid and cell cycle-arrested budding yeast
Size matters in eukaryotic cells. Inability to maintain cell size homeostasis, or the coordination between growth and division, has direct consequences on cellular functions and fitness. Eukaryotic cells have developed mechanisms to ensure proper coordination of biomass accumulation and cell cycle …
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Biosynthetic and Functional Studies of Bacillithiol in Gram-positive Bacteria
… glutathione (GSH) as the dominant LMW thiol in eukaryotic cells as well as Gram-negative bacteria and mycothiol (MSH) as the dominant LMW thiol in Actinobacteria. Functional studies on those thiols have revealed their importance in cell metabolism, also their potential as target for …
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Studies Towards a Total a Synthesis of Tagetitoxin
… a selective inhibitor of RNA polymerase III in eukaryotic cells and RNA polymerase in bacteria. Since its initial isolation and partial characterization, more than thirty years have passed without a fully characterized structure for tagetitoxin. While much research has been carried out on the …
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Biochemical and immunocytochemical analysis of three novel microtubule-associated proteins in Drosophila melanogaster and the rat brain
… are essential organelles found in almost all eukaryotic cells. Some of their diverse functions include intracellular transport, cell division and maintenance of cell shape. Microtubules are composed of highly conserved proteins, the $\alpha$- and $\beta$-tubulins. Many of the functions …
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The use of the broad-host-range plasmid, pTF-FC2, in Agrobacterium tumefaciens mediated plant transformation
… of T-DNA from Agrobacterium tumefaciens to eukaryotic cells is on of the most extensively studied examples of interkingdom DNA transfer. This transfer system has revolutionized plant molecular biology to the extent that the introduction of foreign genes into plant genomes is now a basic …
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