Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"Epitope mapping"”.
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B CELL EPITOPE MAPPING OF RIVAX, A CANDIDATE RICIN VACCINE ANTIGEN
… and hence generating a comprehensive B cell epitope map of ricin toxin would not only help in evaluating future ricin vaccine candidates in humans but also provides an immunological rationale for designing new vaccine candidates. Previous studies have shown that toxin neutralizing antibodies …
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Epitope mapping of the E2 glycoprotein, including the hypervariable region 1, of the hepatitis C virus genotype 3a, in the context of humoral immune pressure
… conserved viral structures and vulnerable HCV epitopes. Twenty genotype 3a serum and plasma samples from patients with chronic HCV infections were obtained from the National Virus Registry Laboratory (NVRL) and from the Molecular Virology Research and Diagnostic Laboratory (MVDRL). HCV genotype …
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Identification and characterisation of HLA-DRB1*0101 restricted CD4+ T cell epitopes in the major grass pollen allergens Phl p 1 and Phl p 5
… was to identify HLA-DR1-restricted CD4+ T cell epitopes of Phl p 1 and Phl p 5 by complementary approaches of epitope mapping: in silico prediction, tetramer guided epitope mapping and human HLA-DR1 transgenics. I investigated recognition of HLA-DR1-restricted CD4+ T cell epitopes in allergic …
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Characterizing and engineering antibodies against the epidermal growth factor receptor
… EGFR signaling have been developed. However, the epitopes of most EGFR antibodies have not been characterized, and the marginal efficacies of current antibodies underscore the need for improved therapeutics. In this thesis work, we have created a novel method of epitope mapping, which is the …
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Genetic characterization of human immunodeficiency virus from Northern South Africa
… inhibitor (NNRTs), co-receptor usage and epitope mapping of HIV-1 isolates from drug naive individuals were investigated. Subtyping was done by phylogenetic analysis making use of ClustalX2 software while drug resistance mutation analysis, co-receptor usage prediction epitope mapping and …
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Development and Characteristics of the Earliest Cross-Neutralizing Antibody Response to HIV-1
… a wide range of clinical isolates), and the epitopes they target, will aid in our understanding of how to elicit such responses by vaccination. Cross-sectional studies of chronic HIV-1 infection have demonstrated that approximately 15% of HIV-1-infected subjects develop such responses. We …
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Linking the structure of alpha-synuclein oligmers to function in Parkinson's disease.
… assays in combination with ESI-IMS-MS. Epitope mapping procedures indicated that different a-syn oligomers have unique epitope features. Dye binding assays such as ThT and ANS fluorescence inferred that the various oligomer types differ in their amyloidogenicity and hydrophobicity. …
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Elucidation of antigenic epitopes on the rubella virus E1 glycoprotein
… viral neutralizing (VN) and hemagglutinin (HA) epitope mapping studies. Deletion mutants of El were constructed from El cDNAs and expressed in in vitro, in COS cells and in E.coli. The mutants were screened with monoclonal antibodies with VN and HA inhibiting activities. The mutants containing …
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Surveillance of Emerging Livestock Viruses
… entry. Therefore, immunodominant neutralizing epitopes of PEDV were identified using a panel of S-specific mAbs. Epitope mapping by peptide ELISA revealed that seven mAbs recognized linear neutralizing epitopes located in the N-terminus of the S2 glycoprotein subunit. Two mAbs recognized a …
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Indagini di spettrometria di massa e proteomica delle proteine dei fluidi corporei dei mammiferi
… MS (Sezione 2B), denominato Intact Transition Epitope Mapping (ITEM)". Lo scopo di questo studio è quello di aprire la possibilità di utilizzare questo metodo ITEM basato sulla MS per rilevare le reattività anticorpali specifiche nel siero di pazienti dopo infezioni da agenti patogeni, …
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A Structural Perspective of Antibody Neutralization of Dengue Virus
… we sought to dissect the relationship between epitope and function. Virologic studies had identified that the most potently neutralizing antibodies are against domain III: DIII) of the E protein. We have identified five epitopes within DENV DIII. Our data suggests that the most potently …
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The Structural Basis of Flaviviridae Interaction with Antibodies and Receptors
… characteristic of flavivirus envelopes and epitope mapping of neutralizing antibodies revealed residues localized to the domain I lateral ridge, fusion loop, domain III lateral ridge and domain I-II hinge. The dimer interface, however, is remarkably small and lacks several contacts present …
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Cell surface display in biomedical applications and biofuels production
… biofuels production. Identification of T cell epitopes is a critical, but often difficult step in developing peptidebased vaccines and T cell immunotherapies. Unlike antibody that recognizes free soluble antigens, T cell receptor (TCR) recognizes its epitope bound to major histocompatibility …
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INFECTION- AND VACCINE-INDUCED IMMUNE RESPONSES TO SARS-COV-2 AND OTHER HUMAN CORONAVIRUSES
… cell responses. Following in vitro expansion and epitope mapping experiments, specific regions in human coronavirus nucleoproteins containing highly immunogenic cross-reactive T cell epitopes were identified. This study underscores the importance of tailored vaccination strategies to optimise …
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CHARACTERIZATION OF DENGUE NEUTRALIZING ANTIBODIES USING RECOMBINANT ANTIBODIES TECHNOLOGY
IN THE LATE 1700S, EDWARD JENNER DESCRIBED INDUCED IMMUNITY IN WHICH HE DEMONSTRATED THAT INOCULATION WITH COWPOX PREVENTED ONE FROM DEVELOPING DISEASE AGAINST SMALLPOX. A CENTURY LATER, EMIL ADOLF VON BEHRING EXPLORED THE POTENTIAL OF PASSIVE IMMUNITY AS AN ANTI-TOXIN THERAPY AGAINST TETANUS. …
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Novel antibody-based biosensors for the detection of haptens, proteins and whole cells
… metal affinity chromatography (IMAC). An epitope mapping study of InlB was also performed, whereby the recombinant protein was portioned into three fragments to estabhsh the relative location of antibody-bindmg epitopes on its structure. The Listeria monocytogenes-derived proteins, …
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Developability assessments and analytical characterization of recombinant protein antigens formulated as low-cost, multi-dose, aluminum-adjuvanted vaccines
… conformational stability of the protein. Epitope mapping studies using HX-MS demonstrated involvement of same helical region of P[4] surrounding the cysteine residue in antigen-antibody binding. In Chapter 4, we further explored the interaction of the NRRV P[4] antigen with various …
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Structure-function of Dengue 2 virus envelope protein domain III with neutralizing antibodies
… by way of physically and biologically mapping the epitopes of DENV ED3-specific neutralizing monoclonal antibodies (MAbs). Site-directed mutagenesis of a DENV-2 recombinant ED3 (rED3) was done in order to map functionally important residues for binding of a panel of 13 ED3-specific …
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Identifikation und Charakterisierung neuer immunogener Proteine und anschließende Generierung rekombinanter Antikörper mittels Phage Display
… der 21 immunogenen Proteine wurden Epitopmappings mit verschiedenen polyklonalen Antikörpern durchgeführt, um immunogene Bereiche näher zu identifizieren und zu charakterisieren. Bei zehn Proteinen wurden lineare Epitope eindeutig mit drei polyklonalen Antikörpern identifiziert, von fünf …
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