Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"EpCAM"”.
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CHARACTERIZATION OF EPCAM IN THYROID CANCER BIOLOGY BY THREE-DIMENSIONAL SPHEROIDS IN VITRO MODEL.
… TICs, Epithelial Cell Adhesion Molecule (EpCAM) has already been defined as a marker for cancer cells with tumor-initiating properties in many solid tumors, where its possible role in tumor progression and aggressiveness has been described. However, this protein is poorly studied in TC and …
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Untersuchungen zur Tumor-spezifischen Glykosilierung des Karzinom-assoziierten epithelialen Adhäsionsmoleküls EpCAM in Kopf-Hals-Karzinomen (SCCHN)
Das epitheliale Adhäsionsmolekül EpCAM wird insbesondere in gesunden Adenoepithelien exprimiert, nicht aber in den mukösen Plattenepithelien der oberen Atem- und Verdauungswege. Aus diesen Geweben hervorgehende Plattenepithelkarzinome dagegen zeigen eine Überexpression von EpCAM. Die Ergebnisse der …
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Lung Progenitor Cell Transplantation for Pulmonary Fibrosis and Ex Vivo Expansion of Patch-Forming EpCAM⁺CD31⁺ Cells
… environment, a significant amplification of EpCAM⁺CD31⁺ progenitors without loss of phenotypic stability or engraftment capacity has been established.</p> <p>These findings lay the foundation for developing safe and reproducible cellular therapies for patients with pulmonary fibrosis.</p>
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Bone Marrow Derived Progenitor Cells and Their Contributions to the Thymic Stroma
… CD45 and the definitive thymic epithelial marker EpCAM, which is also highly enriched in stem cell markers such as, Sca1. CD45+ EpCAM+ cells are present in both the thymus and bone marrow (BM), suggesting that the CD45+EpCAM+ population may be recruited from a hematopoietic derived population in …
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Development of 3D multicellular liver organoids derived from human induced pluripotent stem cells to model antiretroviral therapy induced liver injury
… (SOX17 and GSC) markers. Flow cytometry using EpCAM, CD166 and CD68 antibodies indicated that HLOs comprise of 60,4% EpCAM+ cells, 11,2% EpCAM-/CD166+ cells and 5% EpCAM-/CD68+ cells, respectively. HLOs exhibited high CYP3A4 enzyme activity compared to HepaRG 3D model. Proinflammatory (IL-6, …
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Lymph node and peri-lymph node stroma : phenotype and interaction with T-cells
… expressing the epithelial adhesion molecule EpCAM. Analysis of intra-nodal and extra-nodal lymph node (CD45-) stromal cells through flow cytometry and qPCR provides a general phenotypic profile of the distinct populations. My research focused on the EpCAM+ epithelial cell population located …
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Phenotypic heterogeneity in high-grade serous ovarian cancer: evidence for a stochastic model of proliferation
… strongly express the epithelial surface marker EpCAM. However, previous work in the Brenton and Stingl Laboratories identified a distinct subpopulation of cells with low levels of EpCAM. These cells are uniquely able to regrow tumours in the lab, and are enriched by chemotherapy. The objective of …
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Selective Isolation of Circulating Tumor Cells in Antibody-Functionalized Microsystems
… cells through microchannels functionalized with EpCAM antibodies. The cell attachment ratio was experimentally determined at different flow rates. A modeling system based on Stokesian as well as cell-adhesive dynamics is adopted to analyze the cell motion. The cell motion is modeled as a rigid …
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Digital Profiling of Circulating Extracellular Vesicles at Single-Upconversion Nanoparticle Sensitivity and Resolution
… UCNPs for direct enumeration of single CD9 and EpCAM positive EVs (CD9+EpCAM+EVs). The achieved single-molecule sensitivity results in a femtomolar detection limit (1.8 × 106 EVs mL-1), which was nearly 3 orders of magnitude lower than the standard enzyme-linked immunosorbent assay (ELISA). …
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Protein L Functionalized Microparticles For Early Detection of Surface Markers In Heterogeneous Colorectal Lesions
… 1 (MUC1) and epithelial cell adhesion molecule EpCAM positive tumoroids proportionally to antigen expression, 2) Antibody-Protein L MPs detect CRC surface antigens on the luminal colon surface in vivo, and 3) concurrent targeting of multiple surface antigens enhance sensitivity of detection of …
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Kupplung der DNA-Methyltransferase M.SssI mit Triplehelix-bildenden Oligodesoxynucleotiden
… to silence specific genes. The fact that the EpCAM gene is overexpressed in a variety of carcinome and is involved in carcinogenesis makes it an interesting target. In the first phase of this research, M.SssI was coupled with TFO by native chemical peptide ligation (NCPL), thiazolidine …
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Super-specific DNA methylation by a DNA methyltransferase coupled with a triple helix-forming oligonucleotide
… of the epithelial cell adhesion molecule (EpCAM) with high specificity and affinity. This transmembrane glycoprotein is strongly overexpressed in several carcinomas and its expression levels have been shown to correlate with the EpCAM promoter methylation status (van der Gun et al., 2008). …
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INVESTIGATING THE ROLE OF EXTRACELLULAR VESICLES AND GLYCOSYLATION IN CANCER METASTASIS
… of the well-established breast cancer marker EpCAM. Overall, these results underscore the importance of optimising single-vesicle flow cytometry for comprehensive characterisation of sEVs. They also highlight the HPA lectin binding patterns of breast and colorectal metastatic cell phenotypes, …
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Investigating the role of extracellular vesicles and glycosylation in cancer metastasis
… of the well-established breast cancer marker EpCAM. Overall, these results underscore the importance of optimising single-vesicle flow cytometry for comprehensive characterisation of sEVs. They also highlight the HPA lectin binding patterns of breast and colorectal metastatic cell phenotypes, …
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Humanisierung von Maus-Hybridomantikörpern
… HEA125 (IgG1), der mit hoher Affinität an EpCAM (Epithelial Cell Adhesion Molecule) bindet. Da EpCAM auf der Oberfläche fast aller humaner epithelialer Tumore stark überexprimiert ist, eignet sich HEA125 ggf. als Therapeutikum für Karzinome. Murine Antikörper sind aber nicht in der Lage, …
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Development and evaluation of methodologies for analysis of CTC and ctDNA in patients with ovarian carcinoma
… to target epithelial (cytokeratin (CK) and EpCAM), mesenchymal (vimentin), and OC specific (PAX8) markers for detection of CTCs (Chapter 3). CD45/16 and CD31 were used for the exclusion of white blood and vascular endothelial cells, respectively. PD-L1 was used for CTC characterisation. …
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DISSECTING THE ROLE OF Β-CATENIN AND MYC IN GASTRIC TUMOR FORMATION
… impairment of Epithelial Cell Adhesion Molecule (EpCAM) autophagic degradation promotes β-catenin chromatin accumulation and activation of oncogenic Wnt transcriptional program. Our work reveals an unprecedented signaling framework relevant for Wnt/Myc driven-gastrointestinal tumor development.
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Evaluation of tumour-associated antigens to optically label cutaneous basal cell carcinoma for surgical excision
… in HEK293T cells. The specific binding to EpCAM, EMA, CSPG4, and CD138 antigenexpressing cell lines was observed on incubation with scFvUBS54-SNAP-tag, scFvID405- SNAP-tag, 9.2.27scFv-SNAP-tag, and scFvh-STL002-SNAP-tag, respectively. In addition, we showed the selective cell killing effect …
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Molekulare Studien über zirkulierende Tumorzellen und zellfreie Nukleinsäuren in präklinischen Modellen und Patientenproben
… den Verlust von epithelialen Markern wie z.B. EpCAM oder MUC-1 beinhalten, weshalb ein CTC-Nachweis über epitheliale Oberflächenmoleküle im Vergleich zu antigenunabhängigen Methoden in vivo erfolglos bleibt. Darüber hinaus kann in Übereinstimmung zur klinischen Situation die Induzierung von …
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