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Showing 1 to 20 of 36 for “"ENaC"”.
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The Effects of Accessory Proteins on ENaC Function
… Epithelial sodium channels, also known as ENaC, constitute the rate-limiting step of sodium reabsorption in the distal tubules of the nephron in the kidneys. It is here the final, yet critical, 3% to 5% of sodium reabsorption that dictates blood pressure occurs. In this study, β-ENaC was …
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Renal Epithelial Sodium Channel (ENaC) Regulation of Pregnancy Mediated Hemodynamic Adaptations: Mechanistic Insights
… a renal tubule protein profile indicating α ENaC as the only sodium transport protein upregulated in mid and late pregnancy. To determine the in vivo activity of ENaC we conducted in vivo studies in late pregnant rats (day 18-20) and virgin rats to measure the natriuretic response to ENaC …
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Uncovering the Critical Amino Acids in the Beta Bubunit of the Epithelial Sodium Channel (ENaC)
… channel called the epithelial sodium channel (ENaC). The structure, regulation and assembly of ENaC’s subunits is not yet fully understood. In this study, error prone polymerase chain reaction (EP-PCR) was used to introduce random mutations in the extracellular loop of the beta subunit of ENaC …
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Identification of Accessory Proteins and Their Impact on the Expression of the Epithelial Sodium Channel (ENaC)
The epithelial sodium channel (ENaC) plays a crucial role in ensuring proper Na+ balance in the body. In the nephron, ENaC regulates the final 3-5% reabsorption of Na+ and is a limiting factor for Na+ reabsorption in the kidneys. Because of its role in the kidneys, ENaC is thought to be a critical …
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The development of novel strategies to modulate epithelial sodium channel (ENaC) activity in chronic obstructive pulmonary disease (COPD)
… factor affecting this disorder. Inhibition of ENaC function in the airways has become an attractive therapeutic strategy to counteract airway surface dehydration and defective MCC. Thus, the overarching aim of this research was to investigate the potential of two novel strategies to modulate …
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Einfluss der intrazellulären Cl- Konzentration auf den epithelialen Na+Kanal
Die Regulation des epithelialen Na+-Kanals (ENaC) ist von entscheidender physio-logischer und pathophysiologischer Bedeutung. Neben dem Liddle-Syndrom, einer salz-sensitiven Form des Bluthochdrucks, wird dies besonders bei der Zystischen Fibrose (CF) deutlich. Aufgrund von Mutationen im Cystic …
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Elucidation of the epithelial sodium channel as a salt taste receptor candidate and search for novel salt taste receptor candidates
… for Na+, involving epithelial sodium channel (ENaC). A second rodent transduction pathway, which is triggered by various cations, is amiloride insensitive and not almost understood to date. Studies in primates showed amiloride-sensitive as well as amiloride-insensitive gustatory responses to …
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The role of Serum- and Glucocorticoid kinase in the hormonal control of sodium transport in pulmonary epithelia
… the role of this kinase in dexamethasone-induced ENaC activity in the H441 human bronchiolar cell model. Dexamethasone-deprived cells do not display ENaC activity as there is no amiloride sensitive current in these cells. Groups of dexamethasone-treated H441 cells do display ENaC activity; however …
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Regulation of sodium channel densities in A6 renal epithelial cells by hormonally-dependent mechanisms
Epithelial sodium channels (ENaC) located in the apical membranes of transporting epithelia, such as cells of the renal distal nephron, constitute the rate limiting step in the transcellular transport of sodium. The regulation of sodium ion reabsorption in the kidney, which is essential for salt …
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Regulatory mechanisms of epithelial sodium channel
Epithelial sodium channels (ENaC) are of immense importance, controlling Na+ transport across epithelia and thus playing a critical role in all aspects of fluid clearance as well as numerous other functions. Although extensive studies have been carried out to invistegate the regulation mechanism of …
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Identification of Residues Critical to Epithelial Sodium Channel Assembly
The Epithelial Sodium Channel (ENaC) is a major determinant in fine-tuning the volume and pressure of blood within the cardiovascular system. Understanding the regulation, structural assembly, and arrangement of ENaC is needed. This study describes a method for identifying residues that participate …
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An investigation of the cellular effects of novel agents and multi-drug combinations in cystic fibrosis
… and overactive epithelial sodium channels (ENaC), which leads to hyper-absorption of Na+ and water from the airway lumen. The resulting thickened secretions and airway plugging give rise to devastating cycles of chronic infection and inflammation, leading to a progressive decline in …
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Analysis of the role of the mineralocorticoid receptor in renal principal cell sodium reabsorption in vivo
… with impaired epithelial sodium channel (ENaC) activity in kidney and colon. The aim of the present study was the analysis of the role of renal principal cell MR, which is activated by aldosterone, in ENaC-mediated sodium reabsorption. For this purpose, mice deficient for MR in …
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Hormone-induced expression of the epithelial sodium channel in human airway cells
… through the epithelial sodium channel (ENaC), which is crucial for lung fluid clearance. Although clinical prognosis can be improved by using glucocorticoid hormones to augment the ENaC-dependent removal of liquid from the lungs, we still require a better understanding of the underlying …
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Channel activating proteases: molecular targets for correction of sodium channel dysfunction in cystic fibrosis airways disease
… pulmonary damage and decline. Dysregulation of ENaC, secondary to the loss of functional CFTR in CF, leads to increased Na+ absorption which contributes to airways dehydration, thickened mucus, impaired mucociliary clearance and is thought to be a major contributor to the pathogenesis of lung …
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Valutazione dell'efficienza, efficacia e sicurezza di vettori lentivirali nel trasferimento del gene CFTR in sistemi modello di epitelio respiratorio in fibrosi cistica
… WT gene, or SHRNA molecules directed against ENAC subunits; ENAC is a sodium channel hyperactive in cystic fibrosis.
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The signalling pathways allowing hormonal regulation of Na+ transport in murine collecting duct cells
… of two insulin-sensitizing drugs, TZDs, on ENaC-mediated Na+ transport were investigated and the signalling pathways underlying two other hormonal regulators of ENaC, dexamethasone and vasopressin, were also examined. Unstimulated monolayers of mpkCCDcl4 cells generated spontaneous Na+ …
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Der Mineralokortikoidrezeptor ist keine Voraussetzung für die Stimulation des epithelialen Na+-Transportes durch Glukokortikoide-Messungen an der Mineralokortikoidrezeptor Knock-out Maus
… die Aktivierung des epithelialen Na+ Kanales (ENaC). Diese Wirkung wird beobachtet, wenn Glukokortikoidkonzentrationen die physiologischen Werte überschreiten oder wenn Spezifität-vermittelnde Mechanismen, vor allem die 11-b HSD, inaktviert sind. Dabei war bisher unklar, ob der MR für diese …
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Significance of Protein Interactions in Mediating AF9 Function
… expression of the epithelial sodium channel α (ENaCα) gene in murine renal collecting ducts by modulating the activity of the Dot1l. It has been shown that Af9-Dot1l promotes H3K79 methylation at specific sites in the ENaCα promoter, which then contributes to its repressed state. A number of …
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Study of correlation analysis for protein-protein interactions
… and chloride channels, which included CFTR and ENaC, Clc2 and ENaC, CACC and CFTR, trying to identify the possible networks of sodium and chloride channels in the airway epithelia. This provided us the possible interactions of Clc2 and ENaC γ subunit, CFTR and CACC3. We then applied the same …
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