Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 48 for “"ELISPOT"”.
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Metodika ELISpot a predikce rejekce po transplantaci ledviny.
… před transplantací stanovovali pomocí metody ELISpot (Enzyme-linked immunosorbent spot assay) frekvence aktivovaných T lymfocytů specifických vůči antigenům dárce, které během krátké stimulace (24 hodin) produkovaly IFNγ. Výsledky jsme korelovali s výskytem akutní celulární (ACR) a humorální …
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Evaluating the predictive performance of cytotoxic T lymphocyte epitope prediction tools using Elispot assay data
… complements of Enzyme-linked immunosorbent spot (ELISpot) assays - a very commonly applied immunological technique that is used both to identify regions of pathogen genomes that trigger an immune response and to characterize the relationships between an individual's complement of HLA alleles and …
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Untersuchungen über die antigenspezifische Immunität nach Diphtherie- und Tetanus-Schutzimpfung mit dem B-Zell-ELISPOT-Test
… sowie spezifische Memory-B-Zellen mit dem B-Zell-ELISPOT-Test bei 13 Probanden bestimmt. Es zeigte sich, dass sowohl der Antikörper-Titer als auch die Memory-B-Zellen über lange Zeit nach der Impfung persistieren, ohne jedoch miteinander zu korrelieren. Das spricht dafür, dass der Antikörper-Titer …
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Development of an antigen-specific ELISPOT to detect intestinal antibody responses to the swine whipworm, Trichuris suis
… In this study, an enzyme-linked immunospot (ELISPOT) assay was developed to measure total and antigen-specific IgG and IgA antibody secreting cells (ASC) from gut-associated lymphoid tissues (GALT) [mesenteric lymph node explants from jejunal region of small intestine (SI-MLN) and cecum in …
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Correlates of protective immunity in individuals who are exposed to Hepatitis C but appear uninfected
… and validation of an interferon-? (IFN-?) ELISPOT assay using overlapping peptides (n=441). Using this assay, HCV-specific cellular immune responses were detected in 5/10 (50%) of chronically infected subjects. Responses were identified more frequently, and were directed against more …
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The immunopathogenic mechanism of severe cutaneous adverse reactions to first line anti-tuberculosis drugs
… phenotype validation, Naranjo drug causality and ELISpot assay to identify offending drugs and precision phenotype cases. We isolated DNA from saliva for HLA, ERAP and KIR typing. In a well-defined subset of patients, we used an integrated single-cell approach involving: i) mass cytometry by time …
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Charakterisierung zellulärer Immunantworten von mit SIV immunisierten und infizierten Makaken
… T-Zellimmunantworten mit der bislang verwendeten ELISPOT- Technik. Weiterhin wurden verschiedene T-Zellpopulationen des Blutes und ihre Veränderungen nach SIV-Exposition mit der polychromatischen Durchflusszytometrie charakterisiert. Keine der Immunisierungsstrategien schützte die Impflinge vor …
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Expression and Immunological Characterization of Herv-K Transmembrane Envelope Protein In Human Breast Cancer
… and IFN-γ enzyme-linked immunosorbent spot (ELISPOT) assay. Furthermore, ELISA detected higher titers of anti-HERV-K TM Env IgG antibodies in sera of breast cancer patients. In addition, the magnitude of the anti-HERV TM B cell response was correlated with the disease stage. Peripheral blood …
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Selective allodepletion to improve anti-viral and anti-leukaemic responses after haploidentical transplantation
… the median residual reactivity to host in IFN-γ ELISPOT assays was significantly lower after combined CD25/71 than CD25 allodepletion (14.1 % vs 54.6%, p < 0.05). Third party responses after CD25/71 allodepletion were equivalent to unmanipulated PBMCs in both assays. In pentamer and IFN-γ ELISPOT …
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Discovery of Immunogenic Neo-peptides from Actionable RNA Fusion CD74-NRG1 for Developing Cancer Vaccines
… testing of the 36 peptides using an ELISpot assay revealed that 7 of 10 validated binders to HLA-B*07:02 effectively activated CD8 T cells, indicating their potential to elicit robust immune responses. The dCODE Dextramer assay was used to identify specific immune cell states induced …
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The impact of myeloid derived suppressor cells on vaccine immunogenicity in South African HIV-infected and uninfected mothers and their infants
… proliferation by CFSE and IFN-γ responses using ELISpot assay.
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Der Einfluß von Virusinfektion und Impfung auf autoreaktive T-Lymphozyten bei der Multiplen Sklerose
… wurden, ergab sich weder mit dem sensitiven IFNg-ELISPOT noch mit der quantitativen RT-PCR ein Anhalt für erhöhte Autoimmunreaktivität gegen die zwei untersuchten Myelin-Antigene MBP und MOG. Im Gegensatz dazu konnten mit dem IFNg-ELISPOT-Assay bei einigen gesunden Spendern und MS-Patienten nach …
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Evolution of adaptive immune response from acute to chronic phase of hepatitis C virus infection
… the transmitted founder virus identified via ELISpot. Immune escape was observed in the NGS data set in ~33% of all ELISpot identified epitopes. The majority of HCV-specific CD8+ responses identified via IFN- ELSPOT in chronic progressors were also characterised by a dominant population of …
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NOVEL RNA-BASED CANCER VACCINE FOR AML IMMUNOTHERAPY
… using 102 synthetic peptides was performed in ELISpot assays. Our final 10 candidates were cloned into a vaccine construct. mRNA cancer vaccines were synthesized using our optimized laboratory-scale synthesis pipeline. Using manganese lipid nanoparticles as a delivery agent, the treatment …
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A study of immune responses during antiviral treatment for hepatitis C virus infection
… against HCV overlapping peptides using IFN- ELISpot assays, demonstrating the restoration of HCV specific T cell function following successful viral clearance. There was a distinct difference in baseline ELISpot responses between treatment responders and relapsers, showing a rapid reduction …
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An investigation of the impact of parasitic worm infection on the immunogenicity of candidate HIV vaccines
… responses and histological studies using ELISpot, ELISA, CBA, ICS staining, H&E/CAB staining and hydroxyproline assay. Our findings demonstrated that in a mouse model, chronic Sm-infection induces a predominantly Th2 immune biased response marked with elevated parasite-specific IL-4; IL-6 …
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Investigating cross-clade immune responses in HIV-1 subtype C-infected individuals from South Africa: implications for HIV vaccine design
… observed by IFN-γ production in an ELISpot assay can be observed at physiological concentrations of the peptides and for other functions of HIV-specific T-cells is an important question that remains to be answered. Methods The cross-reactivity of HIV-specific T-cells was assessed …
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An investigation of the effects of helminth worm infection on the capacity of HIV vaccines to boost vaccine-generated immune responses
… vaccination. The magnitudes of cumulative IFN-γ ELISPOT responses to HIV Gag, RT and Env peptides were significantly (p<0.05) lower in the vaccinated and Sm-infected (Vaccine+Sm) mice (948 sfu/106) than vaccinated and uninfected (Vaccine) mice (1733 sfu/106), with IFN-γ responses to RT (CD8) …
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Influence of Anti-CD44 on Murine B Cell Activation
… the inhibitory activity. Additionally, FACS and Elispot revealed that RK3G9-treated cells had reduced numbers of plasma cells. Taken together, these results suggest that CD44 cross-linking could control polyclonal B cell activation by CD40L, but allow sIgM/CD40L activation to continue.
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Investigation of the cancer testis antigen lactate dehydrogenase C as a CD8 T cell target
… epitopes of the LDHC protein by interferon-γ ELISpot. With this approach, T cell clones from one of five patients were identified that were reactive to minimal peptides contained within LDHC. In this patient, the antigenic LDHC peptide differentiated from LDHA by a single amino acid at its …
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