Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 65 for “"Deamination"”.
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INFLAMMATION INDUCED CYTOSINE DEAMINATION DAMAGE IN CANCER ETIOLOGY
… the primary mechanism for these mutations is the deamination of cytosine and cytosine analogs to uracil and the corresponding uracil analogs. During replication, uracil and uracil analogs codes as thymine, leading to the aforementioned transition mutation. However, inflammation is known to cause a …
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Nucleobase deamination as a biomarker of inflammatory processes
… increases ([approximately]30%) in nucleobase deamination products were observed in the SJL mice bearing the RcsX tumor, but the biological meaning of these increases awaits studies of DNA repair kinetics. dO was not detected in any study at levels above 5 per 10⁸ nt, leading to the prediction …
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Single-Stranded Dna Scanning And Deamination With Single-Molecule Resolution
<p>SINGLE-STRANDED DNA SCANNING AND DEAMINATION WITH SINGLE-MOLECULE RESOLUTION</p> <p>by</p> <p>GAYAN SENAVIRATHNE</p> <p>December 2013</p> <p>Advisor: Dr. David Rueda & Dr.Ashok Bhagwat</p> <p>Major: Chemistry (Analytical) </p> <p>Degree: Doctor of Philosophy</p> <p>APOBEC3G (Apo3G) and …
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The chemistry of nitric oxide-induced deamination and cross-linking of DNA
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1997.
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Modulation of Cis-syn Cyclobutane Dimer Formation and Deamination at CpG sites by Nucleosome Particles and Its Implications for Deamination Bypass Mechanism for the Origin of C to T Mutations
… at pH 7 and 37°C over a period of hours or days. Deamination of Cs or mCs in CPDs is highly mutagenic because polymerase ç will faithfully insert A opposite the resulting Us or Ts, thereby producing the observed C to T and CC to TT mutations: the deamination-bypass mechanism). In this thesis, we …
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The Oxidative Deamination of Sulfur Amino Acids by a Bacterial L-Amino Acid Oxidase
<p>The oxidative deamination of sulfur amino acids by a particulate L-amino acid oxidase obtained from an atypical Proteus rettgeri has been investigated. In addition to several sulfur amino acids tested here the enzyme catalyzes the oxidation of L-leucine, L-phenylalanine, L-tryptophan, …
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Nitrosative guanosine deamination : pyrimidine ring opening implications of effects in homogeneous solution as well as anisotropic environments
… two types of chemical reactions with DNA bases, deamination and interstrand cross linking involving the nitrosation of exocyclic amino group of guanine, adenine and cytosine. The ingestion or inhalation of nitrosating reagents (NO[subscript x][superscript -] ) contained in foods, or the …
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Exploring the origins of DNA using synthetic biology Alannah Marie
… from U-DNA to T-DNA was driven by cytosine deamination (Poole et al., 2001), which would lead to mutagenic generation of U in the genome via C to U deamination events. However, a transition to T is an indirect fix to this problem; it enables identification of mutagenic U:G pairs arising from …
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Myers-Inspired Selenium-Mediated Isomerization: An Efficient Generation of Contra-Thermodynamic Allenes and Alkenes with the Potential for Reductive Deamination of Primary Amines and Metal-Free Hydrogenation of Dienes
… may lead to further development in reductive deamination reactions, such as the reduction of primary amines such as anilines, and diamines to monoenes.
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Analysis of the Structural Units of Sulfated Glycosaminoglycans by High Performance Ion Exchange Chromatography
… units of heparin produced by nitrous acid deamination were stoichiometrically reduced with NaB('3)H(,4) and analyzed by high performance ion exchange chromatography to obtain resolution of the four primary disaccharide units of heparin; 4-O-((beta)-D-glucopyranosyluronic …
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Genetic and biochemical studies of human apobec family of proteins
… I reexamined this proposed new role of AID (5mC deamination) with two members of the APOBEC family in a novel Escherichia coli based genetic system. My results confirmed that while all three enzymes are strong cytosine deaminases, only APOBEC3A (A3A) is an efficient deaminator of 5mC. The …
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Weak acid/bases and pH control in upflow anaerobic sludge bed reactors
… mass of H₂CO₃*alkalinity internally due to deamination of proteins to ammonium/ammonia, reduction of sulfates to sulfides and removal of organic acid salts such as potassium bitartrate. Similar to the Iauter tun waste it was of interest to evaluate the effect of recycling on process …
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The Role of Metabolism and Epigenetics in Regulating Cellular Plasticity
… possible substrates derived from oxidation and deamination of 5-methylcytosine—the primary epigenetic mark in DNA. Using an in vitro real-time kinetic assay, we identified that one of the best substrates for three of the four human glycosylases examined is 5-carboxyuracil (5caU). 5caU is the …
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Biochemical and Structural Studies of RNA and DNA Editing Enzymes
… and DNA editing, occurring by way of hydrolytic deamination of cytidine (C) and adenosine (A), is a modification event that profoundly effects on an organisms vitality and quality of life. This study focuses on two types of enzymes---ADAT (adenosine deaminase acting on tRNA) and APOBEC3G …
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Structure Function Analysis of Adenosine Deaminase Acting on tRNA (ADAT2) from Trypanosoma Brucei
… of the genetic code. The enzymes responsible for deamination of adenosine 34 to inosine are found in all organisms and in all but a few cases are essential for life. In bacteria the reaction is carried out by the homodimeric adenosine deaminase acting on tRNA (ADAT) and modifies only tRNAarg, …
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Synthesis and applications of aminated biopolymers
… (PLP) for application in non-enzymatic deamination of L-phenylalanine to phenylpyruvate. An ionic complex between PLP and Q188 was formed and successfully utilised to convert L- phenylalanine into phenylpyruvate, however, the polymer complex showed a decline in activity over time …
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The Growth and Bioenergetics of <i>Callinectes sapidus</i> Larvae and the Effects of Diet Quality on Larval Physiology
… oxygen. Excretion is a function of amino acid deamination and probably did not differ between diets because protein levels were held relatively constant.</p>
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DEVELOPMENT OF 4'-ETHYNYL-2'-DEOXYCYTIDINE (EdC), A REPLICATION-STRESS INDUCING NUCLEOSIDE ANALOG PRODRUG WITH PREFERENTIAL ACTIVITY IN LEUKEMIA AND LYMPHOMA SUBTYPES
… dCK. Remarkably, EdC was resistant to cytidine deamination and SAMHD1 metabolism mechanisms and exhibited higher potency against ALL compared to FDA approved nelarabine. Finally, EdC was highly effective against DLBCL tumors and B-ALL in vivo. These data characterize EdC as a pre-clinical …
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Evaluation of the mechanisms of neurotoxcity using metabolomic strategies
… metabolites particularly those related to DNA deamination and methylation pathways. One of the increased metabolites was uracil which can be generated from DNA deamination or from fragmentation of RNA. To investigate the origin of uracil genomic DNA was isolated and analysed by LC-MS. …
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The Role of 5-Hydroxymethylcytosine in Genome Instability
… 5hmC-mediated genotoxicity depends on its deamination within the nucleotide pool, prior to its incorporation within genomic DNA. This is validated by the observation that loss of the SMUG1 DNA glycosylase, which excises genomic 5hmU, rescues the hypersensitivity of BER-deficient Polb-/- …
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