Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 52 for “"DNASE I"”.
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Assessment of chromatin activity in mouse and human tissues
… of the cell cycle including mitosis. Recently, a DNAse I-directed in ~ nick translation technique has been used to demonstrate differences in the DNAse I sensitivity of euchromatic and heterochromatic regions of mitotic chromosomes. This ill ~ technique has been used in this study to ask whether …
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Identification of seminal proteins related to fertility of bulls
… structure homology to a recently described novel DNase I-like protein. The physiological significance of the similarities between FAA and a protein homologous to DNase I is unknown at this time as no function has been described for the DNase I-like protein. The second study describes the isolation …
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Manufacturing GMP-grade mRNA vaccine-associated Enzymes: Bioprocess Development and Economics
… RNA polymerase (T7RNAP), RNase Inhibitor (RI), DNase I, Vaccinia Capping Enzyme (VCE), Inorganic Pyrophosphatase (iPPase), and 2’-O-methyltransferase (2OMT), commercially in South Africa under GMP conditions. A four-phase techno-economic methodology was employed, integrating bioprocess …
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Deoxyribonuclease probing on sea urchin embryo chromatin
… nuclease, pancreatic deoxyribonuclease (DNase I) and restriction endonucleases. Micrococcal nuclease and restriction endonuclease digestions of sea urchin gastrula chromatin have been analysed and it is shown that it is not possible to isolate large polynucleosomal chromatin complexes …
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Poly(glutamic acid) promoted assembly of nucleosome cores on the histone gene quintet of psammechinus miliaris
… resistance to Micrococcal nuclease digestion, DNase I digestion patterns, DNase I digestion kinetics at the susceptible sites, electronmicroscopic appearance, hi stone content and electrophoretic mobility. Cores were also assembled on unique DNA, namely the intact h22 histone quintet of …
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The association of bovine parvovirus DNA and proteins with the nuclear matrix of infected cells
… nuclei to high salt conditions and DNase I. Protein analysis of the two matrix types showed the polypeptide composition to be similar. Both procedures showed enrichment for BPV DNA with progressive DNase I digestion. Over the course of infection the amount of BPV DNA associated with …
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Identification and Characterization of the Cis-acting Elements Around the Murine cd4 Cnhancer
… reveals 11 regions of homology. There is also a DNase I hypersensitive (DH) site located immediately upstream of the 5' enhancer. These data suggest that the enhancer may be a part of a larger control element of deletions (3' and 5' direction) covering a 1158 bp region containing the enhancer and …
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Genetic and Biochemical Studies of YqjH and YqjI: Cross-talk between Iron Homeostasis and Nickel Homeostasis in Escherichia Coli
… optimizing the protocol for fluorescence-labeled DNase I footprinting, we improved the data quality and reproducibility, which allows it to be used for the quantification of protein-DNA binding affinity. We then used this technique to characterize the mechanism of YqjI regulation in the yqjH-yqjI …
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DNA Triplexes in chemistry biology and medicine
… (Tm) was as follows: MeP > MeC > MePOMe > MeCAE. DNase I footprinting studies indicate that at pH 7.5, MeP, when incorporated into the TFO, enhances the stability of the triplex by three-fold relative to MeC. In addition, UV melting, DNase I footprinting, and gel electrophoresis studies were …
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Purification and characterization of a poly(dG).poly(dC)-binding protein from Parenchinus angulosis
… gel slice, by Southwestern blotting and by DNase I footprinting. Ultraviolet crosslinking of the nuclear extract and purified suGF1 revealed the presence of the same specific bands on SDS-P AGE. Optimal suGF1 DNA-binding was shown to occur at relatively high ionic strength (175 mM). suGF1 …
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Control of major histocompatibility Class I antigen expression in adenovirus transformed cells.
… was investigated in Ad-transformed cells by DNase I footprinting. The factors RXRbeta/H-2RIIBP which bind to this sequence are members of the ER/TR subfamily of steroid/thyroid hormone receptors. Members of this subfamily have the sequence AGGTCA conserved in their binding sites; this …
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The Impact of Selected Gene Mutations to the Activation and DNA Binding Activity of the Transcription Factor VraR
… and S164A binding activity to the PvraSR by DNase-I footprinting. Finally, in vivo studies on the effects of substitutions of the above residues on the mRNA level of a number of VraSR regulon genes (fmtA, pbp2, and sgtb) provide insights as to how substitutions at certain positions may be …
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Transcriptional regulation of basal and phenobarbital-mediated expression of rat cytochrome 2B genes
… no protection was detected in these regions by DNase I footprinting assay. Instead, a region (FP1) from $-$64 to $-$45 was protected by liver nuclear extracts. Mutation of either the BTE or FP1 sequences of CYP2B1 reduced transcriptional activity in HepG2 cells. FP1 was identified as a …
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Cloning a Putative DNA-Binding Protein Controlling the 5'LTR of the Copia Element in Drosophila
… identified by mobilityshift binding assays and DNase I footprinting assays. The data reveals three protected regions: a TATA-binding site, the AT-1, and AT-2 binding sites. The TATA-binding site and AT-1 site are located within the 5'LTR, while the AT-2 sites is located within the 5'UTR of copia …
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Computational Inference of Genome-Wide Protein-DNA Interactions Using High-Throughput Genomic Data
… ChIP-seq, etc.), nuclease digestion methods (DNase-seq, MNase-seq, etc.), and others. However, a single experimental technique often only provides partial and noisy information about the whole picture of protein-DNA interactions. Therefore, the overarching goal of this dissertation is to …
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The study of transcriptional regulation of necrotrophic effector genes ToxA and Tox3 in the wheat pathogen Parastagonospora nodorum
… other than TATA box on the ToxA promoter. DNase I footprinting and yeast one-hybrid assay also did not identify protein candidates interacting with the ToxA promoter. Interestingly, the ToxA promoter showed ability to induce strong expression in the tested media after replacing the ToxA …
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The study of transcriptional regulation of necrotrophic effector genes ToxA and Tox3 in the wheat pathogen Parastagonospora nodorum
… other than TATA box on the ToxA promoter. DNase I footprinting and yeast one-hybrid assay also did not identify protein candidates interacting with the ToxA promoter. Interestingly, the ToxA promoter showed ability to induce strong expression in the tested media after replacing the ToxA …
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