Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"DNA chip"”.
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DNA chip designed antisense oligodeoxynucleotides targeting EGFR MRNA for brain tumour therapy
… Oligodeoxynucleotides (ODNs) are small pieces of DNA that are often modified to increase their stability to nucleases and can be targeted to the aberrant gene in order to inhibit it and thus prevent its transcription into protein. By specifically binding to mRNA in an antisense manner, they can …
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A label-free, fluorescence based assay for microarray
DNA chip technology has drawn tremendous attention since it emerged in the mid 90 s as a method that expedites gene sequencing by over 100-fold. DNA chip, also called DNA microarray, is a combinatorial technology in which different single-stranded DNA (ssDNA) molecules of known sequences are …
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Reproducibility and reliability of chromatin immunoprecipitation in clinical research
<p>Association between histones and DNA is crucial for many cellular functions such as gene transcription and epigenetic silencing. Changes to chromatin structure influence gene expression via histone modifications. Chromatin immunoprecipitation (ChIP) is an experimental technique used to …
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Studien der Genexpressionsänderung während der Entwicklung von Drosophila melanogaster mittels DNA-Microarrays
… identifizieren wurde im Rahmen dieser Arbeit ein DNA-Microarray entwickelt, mit dem Veränderungen in der mRNA-Expression analysiert wurden. Auf der Grundlage einer neuentwickelten ab initio Genvorhersage im Rahmen einer Koorporation wurden spezifische Primer generiert, die eine Amplifikation der …
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Understanding HNF-1β through Identification of Interacting Proteins and Target Genes in the Kidney
… in the kidney. Chromatin immunoprecipitaion and DNA chip analysis (ChIP-chip) were performed to identify direct target genes of HNF-1Beta in the kidney. Phosphodiesterase 4C (PDE4C) was identified as an HNF-1Beta target gene. PDE4C belongs to the phosphodiesterase superfamily of enzymes that …
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Identifizierung und Charakterisierung von Zielgenen des Epstein-Barr Virus nukleären Antigens 2 (EBNA2)
… und einer umfassenden mRNA-Expressions-Analyse (DNA-Chip-Hybridisierung) konditional oder permanent MYC- oder EBV/EBNA2-abhängig proliferierender Zellen. Die erhaltenen Daten bestätigen, dass die von EBNA2 und MYC gemeinsam induzierten Zielgene in grundlegende Prozesse der Lebenserhaltung wie den …
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3D-DNA-Chips : oberflächengebundene funktionelle Polymernetzwerke als Matrix für Nukleinsäure-Microarrays
… so beschichtet, dass sie als Substrate für DNA-Chips eingesetzt werden können. Die photovernetzbaren Polymere basieren auf N,N?-Dimethylacrylamid als Monomer und sind über radikalische Polymerisation hergestellt worden. Indem geeignete Monomere copolymerisiert werden, können Ladungen, …
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Analysis of the genome organization and fitness traits of non-pathogenic Escherichia coli strain Nissle 1917 (O6:K5:H1)
… other molecular biological methods such as PCR, DNA-chip technology, subtractive hybridization, transcriptomics and proteomics are of increasing importance for research on infectious diseases and public health. The aim of this work was to characterize the genome structure and -content of the …
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A study on endocrine disrupters in the environment through the microarray technology
… The aim of my work is to produce a microarray DNA chip for the investigation of the estrogenic activity of different compounds present in the environment. The chip will consist of a selection of 100 genes that are estrogen responsive and it will cover the spectrum of activities of estrogenic …
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Studies on the architecture and on transferability of pathogenicity islands of uropathogenic Escherichia coli strain 536
… The continously growing amount of bacterial DNA sequence information gives us also the possibility to gain deeper insight into bacterial pathogenesis. With the help of comparative genomics, microbiological research can focus on those DNA sequences that are present in pathogenic bacteria but …
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Identification of genetic markers associated with growth and morphology quality in senegalese sole (solea senegalensis) to boost aquaculture production
… to growth traits was carried out. A low-density DNA chip was designed and validated for 49 SNPs distributed in 17 SseLGs. The analysis of fast and slow-growing families identified two significant markers within the general transcription factor 3C polypeptide 4 and the mitochondrial fission …
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Analyse von Single Nucleotide Polymorphisms an Glas-Oberflächen
… Affinitätssensor bzw. ein Durchfluss-Biochip-Scanner mit integrierter Fluoreszenzdetektion verwendet. An den immobilisierten Analyten (PCR-Produkten) wurde eine Fluoreszenzoligonukleotidsonde hybridisiert und anschließend die Dissoziation der Sonde im Fluss verfolgt. Die Diskriminierung …