Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"DNA Amplification"”.
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Multiplexed label-free electrical detection of DNA amplification using field effect transistors
… research project was to develop a miniaturized DNA amplification biosensor for the detection and identification of pathogenic bacteria. Using tailored loop-mediated isothermal amplification (LAMP) and field effect transistors, we developed a microchip platform for multiplexed screening of …
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Realaus laiko PGR, taikomos patogeninių bakterijų identifikavimui maisto matricoje, optimizavimas /
… chicken wing rinses and increase of the DNA amplification efficiency by using PCR additives (ethylene glycol and bovine serum albumin) in the reaction mixture. The lowest possible detection threshold is 1 CFU ml-1 while growing each bacterium separately for optimally 6 hours. Influence of …
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Molecular analysis of herpes simplex virus type 1 latency in experimentally infected mice / Barry Slobedman.
… whether latent HSV genomes are a result of viral DNA amplification in the PNS during the establishment phase and to investigate the relationship between HSV DNA copy number and viral transcriptional activity during latent infection of the PNS. In order to map the distribution of viral nucleic acid …
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Development of a Multi-Component Biosensor for Recombinase Polymerase Amplification-CRISPR/Cas12a-Based Detection of Fusarium oxysporum f. sp. cubense tropical race 4
… for sensitive and portable detection of Foc TR4 DNA. The system combines microneedle-based DNA extraction, Recombinase Polymerase Amplification (RPA), Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas12a-mediated detection, and electrochemical square wave voltammetry (SWV) …
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RAPID COLORIMETRIC IDENTIFICATION OF ESCHERICHIA COLI SHIGA TOXINS 1 AND 2, AS WELL AS EAE AND AGGR, USING LOOP MEDIATED ISOTHERMAL AMPLIFICATION FOLLOWED BY PNA/AUNP DETECTION
… coli</em>.<em> </em>This research utilizes a DNA amplification technique called Loop-Mediated Isothermal Amplification (LAMP) to detect different strains of pathogenic <em>E. coli</em>. Common visualization methods of LAMP amplicons include using LAMP reaction buffer containing a pH indictor …
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Metal-Organic Framework-Based Biosensors for Enzyme-Linked Assays
… frameworks (MOFs) as biosensors for monitoring DNA amplification in LAMP processes, with a specific focus on detecting phosphate compounds like deoxynucleotide triphosphates (dNTPs) and pyrophosphate (PPi). The findings demonstrate that Eu(BTC) and TbEu(BTC) MOFs can effectively differentiate …
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Hubungan kekerabatan beberapa kultivar pisang (Musa sp.) untuk sifat ketahanan terhadap penyakit berdasarkan Resistance Gene Analog (RGA)
… adalah kultivar Embug, untuk dibandingkan pita DNA dengan 9 kultivar lain. Primer yang digunakan adalah primer daerah terkonservasi NBS-LRR dan NLRR. Tahap Penelitian meliputi ekstraksi DNA, Amplifikasi DNA dan pembuatan dendogram dengan softwere NTSys 2.01.Parameter data dalam penelitian ini …
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Chromosomal localization of a proinsulin transgene inserted with a transposon-based vector into Japanese quail, Coturnix coturnix
… a reproducible method of detecting stable DNA insertion into Japanese quail and provide a method for gene location on avian chromosomes. This research resulted in the development of a different method of obtaining chromosome spreads in Japanese quail, the establishment of primed in situ …
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Ultrasensitive DNA aptasensors based on exponential amplification reaction
… a general method to enhance the sensitivity of DNA aptasensors by coupling them with an isothermal DNA amplification technique known as exponential amplification reaction (EXPAR). The method involves two steps: (1) first, the target-induced structure-switching of DNA aptamers causes the release …
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THE ROLE OF MTHFR GENE MUTATION IN NEURODEVELOPMENTAL DISORDERS: EXPLORING POTENTIAL CORRELATIONS WITH ATTENTION DEFICIT HYPERACTIVITY DISORDER AND ANXIETY
… to ADHD and anxiety. A group of 80 participants’ DNA samples were collected through cheek swabs following IRB approval. Participants completed several questionnaires to assess their signs and symptoms of ADHD. DNA amplification of the MTHFR gene was achieved using loop-mediated isothermal …
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Efficient Synergistic De Novo Co-Assembly Of Bacterial Genomes From Single Cells Using Colored De Bruijn Graph
<p>Recent progress in DNA amplification techniques, particularly multiple displacement</p> <p>amplification (MDA), has made it possible to sequence and assemble bacterial</p> <p>genomes from a single cell. However, the quality of single cell genome assembly has</p> <p>not yet reached the quality of …
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Molecular typing of virulence genes in enterotoxigenic Bacillus cereus
… dye and its florescence increases as a result of DNA amplification during real-time PCR. A second-derivative plot is obtained at the end of the PCR run, where amplicons are differentiated based on their DNA melting temperature (Tm). DNA was extracted from Tryptic Soy Broth (TSB) and 2.5% Nonfat …
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Determination of soybean cultivar resistance to soybean cyst nematode with quantitative polymerase chain reaction
… the roots were surface sterilized and DNA was extracted with the DNA FastKit (MP Biomedicals, Santa Ana, CA)). For the qPCR assay, primer pair for single copy gene HgSNO, which codes for a protein involved in the production of vitamin B6, was selected for H. glycines DNA amplification …
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Chloroplasts as bioreactors : high-yield production of active bacteriolytic protein antibiotics
… vector (pTox) was constructed to allow DNA amplification in bacteria. The construction of the pTox transformation vector allowing a recombinase-mediated deletion of an E. coli transcription block in the chloroplast, leading to an increase of foreign protein accumulation to up to 40% of …
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Molecular characterization of the putative oncogene myeov
… identified using the tumorigenicity assay with DNA from a patient suffering a gastric carcinoma. The Myeov gene is localized at chromosome band 11q13, a frequent site for chromosomal rearrangements in various carcinomas and B-cell neoplasms. The gene was shown to be involved in cases of multiple …
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Experimental and numerical study of temperature-actuated droplets within microfluidics
… works of performance optimization of on-chip DNA amplification devices and encapsulating bacteria and live cells in agarose droplets for drug delivery applications.
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Comparative Phylogeography of Central African Duikers Using Non-invasive Sampling Methods
… needed to be addressed prior to large scale DNA amplification. First, the best storage method for obtaining DNA from fecal samples needed to be established. Our study revealed that while silica is best for nuclear microsatellite analyses, RNA<em>later</em> is the best storage medium for …
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Micro electrochemical sensors and PCR systems: cellular and molecular tools for wine yeast analysis
… of so-called Lab-on-a-Chip (LOC) systems for DNA analysis mostly applied to clinical diagnosis. This thesis deals with the investigation of two miniaturized devices – a cell-based biosensor and a DNA amplification system – for the cellular and molecular analysis of wine yeasts, …
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Genome analysis: mutation analysis using near infrared laser-induced fluorescence (NIR-LIF) and single molecule detection in microfluidic devices
… each of which has its own unique advantage. DNA amplification via polymerase chain reaction (PCR) provides an unlimited supply of material for subsequent genetic analysis even in case where only a single copy of the DNA molecule is present in the sample. Research presented in this …
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