Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 56 for “"Cx43"”.
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FATE OF Cx43 IN CARDIAC AND EPIDERMAL TISSUES HARBOURING A DISEASE-LINKED Cx43 MUTANT
Mutations in the GJA1 gene encoding Cx43 are linked to oculodentodigital dysplasia (ODDD), a pleiotropic, autosomal dominant disorder. We hypothesized that in the Gjal54 mouse model of ODDD there would be significant down-regulation of total Cx43 yet minimal compromise to cardiomyocyte or …
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Dysregulation of connexin-43 (cx43) in doxorubicin-induced cardiotoxicity
… the expression and localisation of connexin-43 (Cx43) in rodent and human cardiac cells and cells of the vasculature to identify if drug treatment impacts this important cardiac connexin. Through investigating the cardiotoxic effects of DOX, this project has shown that total Cx43 protein levels …
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Role of mural Cx43 in vascular interactions and its regulation by breast tumor cells
… We demonstrate that heterotypic connexin 43 (Cx43)-dependent gap junction activity is required for mural cell-induced quiescence of endothelial cells, and that long term exposure of mural cells to media conditioned by aggressive MDA-MB-231 breast tumor cells downregulates Cx43 mRNA and protein …
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Internally Translated Cx43 Isoform GJA1-20k Affects Epithelial to Mesenchymal Transition and Metastatic Cancer Cell Behavior
… EMT. The gene GJA1 encodes for Connexin43 (Cx43), the most ubiquitously expressed gap junction protein where altered regulation of Cx43 is associated with cancer progression. Intriguingly, Cx43 mRNA undergoes alternative ‘internal’ translation initiation, generating N-terminally truncated …
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The Cx43 Carboxyl-Terminal Mimetic Peptide αCT1 Protects Endothelial Barrier Function in a ZO1 Binding-Competent Manner
The Cx43 CT mimetic peptide, αCT1, originally designed to bind to ZO1 and thereby inhibit Cx43/ZO1 interaction, was used as a tool to probe the role of Cx43/ZO1 association in regulation of epithelial/endothelial barrier function. Using both in vitro and ex vivo methods of barrier function …
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The role of conexin 43 in murine sertoli cell development
… testicular gap junction protein, connexin 43 (CX43), in the thyroid hormone effect on Sertoli cell development. To understand the specific role of CX43 in Sertoli cell development, I generated an animal model in which Cx43 is specifically deleted in Sertoli cells to circumvent the neonatal …
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Investigating viral subversion of intercellular communication
… The gap junction protein connexin43 (Cx43; gene name – GJA1) is the most widely expressed human connexin protein and is the predominant connexin in the working myocardium. Given the immunological role for Cx43 gap junctions, we hypothesized that gap junctions would be targeted during …
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Phosphorylation kinetics of cardiac gap junction regulation during stress
… junctions consisting primarily of connexin43 (Cx43) connect cardiomyocytes at regions of contact between cells known as the intercalated disc to facilitate cellular coupling. Cardiac pathologies frequently manifest with disrupted gap junctional intercellular communication which can generate …
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Single channel studies on the modulation of connexin hemichannels by Cx43 targeting peptides and cytoplasmic Ca2+ in the context of heart disease
… first and second extracellular loop regions of Cx43. Intriguingly, despite the growing interest and wide use of connexin mimetic peptides in hemichannel studies, no conclusive data and arguments are available to support a direct action of these substances on hemichannels. In chapter III, we …
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A translational approach to understanding cellular responses to vascular injury
… is poorly understood. Here, I hypothesized that Cx43 and its functional regulation by kinases play a role in vascular cell injury response. To investigate this, I used human vascular tissue from coronary artery bypass grafts and mouse models of ligation induced vascular injury. First, I …
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Enhancing spinal cord repair with connexin43 antisense oligodeoxynucleotides
… inflammation, and scarring. Connexin43 (Cx43) gap junctions and hemichannels contribute significantly to spinal cord injury by remaining open in dying cells, spreading toxic substances to neighbouring cells and the surrounding extracellular environment, and exacerbating inflammation. …
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The Role of Connexin43 and Gap Junctional Intercellular Communication in the Apoptotic Process of Auditory Cells
… of gap junctional activity with either Cx43 siRNA transfection or a pharmacological drug, 18α-glycyrrhetinic acid in HEI-OC1 auditory cells caused a marked increase in cell viability with a decrease of apoptosis based on cleaved Poly (ADP-ribose) polymerase (PARP) and cleaved caspase 3 …
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Mutations in Connexin43 and Connexin40 Associated with Human Atrial Fibrillation; Physiology and Mechanism
… highly expressed cardiac connexin genes, GJA1 (Cx43) and GJA5 (Cx40), have been identified by our group in a cohort of patients with lone AF. To characterize trafficking of a novel somatic frameshift Cx43 mutant and three novel Cx40 mutants, fluorescently tagged connexins were visualized in HeLa …
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Alternative mechanisms of translation initiation in modulation of gap junctional coupling
… and immunological coupling. Connexin43 (Cx43, gene name GJA1) is the most ubiquitously expressed gap junction protein, and Cx43 gap junctions are altered in pathological states including cardiac disease and cancer. The GJA1 mRNA undergoes alternative translation initiation to yield a …
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Role of Astrocyte Network in Edema after Juvenile Traumatic Brain Injury
… process: Aquaporin 4 (AQP4) and Connexin 43 (Cx43). AQP4 is expressed extensively in astrocyte endfeet, which surrounds the blood vessels as part of the blood brain barrier (BBB). Cx43 is central in astrocyte to astrocyte connection and communication. We hypothesized that AQP4 acted as one of …
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Connexin43 as a novel therapeutic target in age-related macular degeneration
… inflammation and vascular changes. Connexin43 (Cx43) is the most ubiquitous gap junction protein in mammals playing a role in intercellular communication. Increased Cx43 expression in the vasculature and astrocytes has been reported for many human central nervous system injuries. However, the …
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Submembrane cytoskeleton-regulated assembly and functional activity of gap junctions
… identifiziert.Das Auftreten von Connexin43 (Cx43) in der Plasmamembran hängt von der funktionellen Aktivität der kleinen GTPasen Sar1 und ARF1 ab. Der Transport von Cx43 vom endoplasmatischen Retikulum zum Golgi-Apparat wurde durch die Expression einer GTP-restrikten Mutante des Sar1 …
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Mechanisms of Intercellular Communication During Breast Cancer Progression Through Metastasis
… EMT is gap junctions composed of connexin43 (Cx43), however, this is paired with an increase in expression of cytoplasmic Cx43 which binds microtubules. To elucidate the role of cytoplasmic Cx43 during EMT and breast cancer metastasis, we utilize a Cx43 mutant that has reduced binding with …
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Gatekeeper Connexin43 Phosphorylation Events Regulate Cardiac Gap Junction Coupling During Stress
… heartbeat. Gap junctions comprising primarily Cx43 reside within the intercalated discs connecting cardiomyocytes, effecting not only direct intercellular electrical coupling, but the localization of other junctional structures and ion channels. Alterations in Cx43 expression occur in …
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Implication des voies de signalisation associées au calcium dans la régulation des jonctions communicantes au niveau testiculaire
… rongeurs (21 chez l'humain), la connexine 43 (Cx43, aussi appelée Gja1, pour Gap junction en anglais) est la connexine la plus abondante dans le système reproducteur masculin. Dans notre laboratoire, nous avons identifié plusieurs facteurs de transcription participant à la régulation de …
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