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Showing 1 to 20 of 118 for “"Column chromatography"”.
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Separation of 2,3-Butylene Glycol and Acetoin in Fermented Cheese Whey Permeate by Liquid Column Chromatography
… here involves utilizing a low cost liquid column chromatographic operation for separating 2,3-butylene glycol and acetoin (another major by-product of the fermentation), in fermented cheese whey permeate. Due to their similarities, acetoin (2-hydroxy-3-butanone) and 2,3-butanediol have been …
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The measurement and modelling of the intracrystalline diffusion of cyclohexane in ZSM-5 using zero length column chromatography
… presents an investigation, using the zero length column (ZLC) method, into changes in the diffusional properties of cyclohexane in ZSM-5 that has had tetraethoxysilane deposited on the surface in the liquid phase. Theoretical analyses of the ZLC technique and its use in a detailed study of …
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The Fractionation of Crude Petroleum using Chromatography, Extrography and Supercritical Fluid Extraction
… In this work the fractionation selectivity of column chromatography, extrography and supercritical fluid extraction was compared.<br/> <br/>Column chromatography with a silica gel as stationary phase provided a means of fractionating a standard mixture of saturated and aromatic hydrocarbons and …
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Vitamin A and glycoproteins of rat corneal epithelium
… eluted with 0.4 N LiCl solution by a stepwise column chromatography on DEAE-Sephadex A-50. A specific "peak S" glycopeptide eluted between 0.35 N and 0.42 N LiCl solution was found to be the most decreased (approximately by 50%) glycopeptide component by vitamin A deficiency when a new …
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DNA and RNA synthesis in leaves during TYMV replication
1. Small scale HAP column chromatography has been developed as a rapid and reproducible method for the isolation of TYMV-specific DS-RNA. 2. A procedure for the isolation of an outer chloroplast membrane fraction from healthy and diseased Chinese cabbage has been developed. This membrane system …
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Characterization of Transfer RNA Associated with Plasma Membranes
… chromatographed using Benzoylated DEAE-Cellulose column chromatography. No major difference was found between cytoplasmic and membrane isoaccepting species for lysine and arginine transfer RNAs.
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The Fruit of Poncirius Trifoliata Promotes Mesenchymal Stem Cell Differentiation into Osteoblasts in Glucocorticoid-induced Osteoporosis Model
… acetate extract of poncirus trifoliata through column chromatography over a silica gel eluting in gradient system and combined based on their thin layer chromatography (TLC) pattern. Fourth subfraction (E4) of ethyl acetate extract was further purified by column chromatography over MCI gel …
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A preliminary analysis of the lipid contents of a biofilm from a Yellowstone hydrothermal pool
… precipitation, mild alkaline methanolysis and column chromatography to isolate different fractions of the TLE. Compounds were then identified by gas chromatography-mass spectrometry (GCMS). The mild alkaline methanolysis allowed for the identification of decanoic acid methyl ester, a fatty acid …
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ΣΥΝΘΕΣΗ ΚΑΙ ΜΕΛΕΤΗ ΤΩΝ ΙΔΙΟΤΗΤΩΝ ΝΕΩΝ ΙΩΔΟ-ΥΛΙΔΙΩΝ Β-ΔΙΣΟΥΛΦΟΝΩΝ
… CONDITIONS. THE PRODUCTS ARE ISOLATED USUALLY BY COLUMN CHROMATOGRAPHY. WHEN AN IODONIUM YLIDE WAS HEATED OR IRRADIATED PROBABLY DISSOCIATED INTO IODOBENZYNE AND BIS(PHENYLSULFONYL) CARBENE. THE THERMAL AND PHOTOCHEMICAL STABILITY OF I-YLIDES OF B-DISULFONES AND THEIR REACTIONS WITH HETEROCYCLIC …
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The metabolism of adenosine nucleotides in thoracic muscle mitochondria from the American cockroach
… treatment, ammonium sulfate fractionation and column chromatography on G-75 sephadex. Some properties of the enzyme were determined. These include: a pH optimum of 5.8, an optimum Mg concentration of 3 x 10⁻³ M, a substrate specificity for ADP, and an equilibrium constant of 0.44. In addition, …
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Biochemical characterization of nitrate transport at the plasma membrane of plant cells using isolated vesicles
… and $\sp{36}$ClO$\sb4\sp{-}$) by aqueous column chromatography in Sephadex G-10, allows for the recovery of about 80% of the initial radioactivity as $\sp{36}$ClO$\sb3\sp{-}$. This radiolabeled anion was used as a tracer for nitrate in membrane transport studies with isolated plasma …
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Studies in the Folate Metabolism of Rats
… of the urinary metabolites were characterized by column chromatography. The effects of an oral dose of methotrexate on folic acid metabolism in adult male Wistar rats were studied. An oral dose of 10 mg/kg body weight methotrexate was administered 24 hours before 2-14C folic acid (76 microgram/kg …
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Expression & Purification of Recombinant Plasmodium falciparum Erythrocyte-binding Ligands
… proteins in COS-1 cells. Purification by column chromatography yielded 0.52 mg of BAEBL and 0.433 mg of JESEBL. The production and purification of these recombinant hexahistidyl proteins can allow for future binding affinity and kinetic analysis that may eventually define the contributive …
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Synthesis of Resveratrol Ester Derivatives
… have been synthesized and purified once by column chromatography.</p>
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Variation in proline-rich proteins and DNA methylation patterns in soybean seed color isolines
… coats using insoluble polyvinylpolypyrrolidone column chromatography. The expression of a similar seed coat protein, PRP2, was not affected by seed color genotype. Thus, a mutation in the anthocyanin pathway appears to affect a specific cell wall protein, PRP1.
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Funkcionalizuotų alkiloligoetilenoksidų sintezė ir tyrimas /
… of several steps and isolation method without column chromatography for the synthesis of thiolipids were also discovered. New functionalized biotins were synthesized for tethering of biological objects. Vibrational bands of new 4,4ʹ-disubstitute-1,2-ditholanes were assigned by the theoretical …
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Synthesis and investigation of functionalized alkyl oligo(ethylene oxides) /
… of several steps and isolation method without column chromatography for the synthesis of thiolipids were also discovered. New functionalized biotins were synthesized for tethering of biological objects. Vibrational bands of new 4,4ʹ-disubstitute-1,2-ditholanes were assigned by the theoretical …
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Study of the reaction of nitriles with dialkyl phosphites for synthesis of a-Amino-bis-phosphonates
… by 1H and 13C NMR), although purification by column chromatography was ineffective. Formation of the addition products in the remaining reactions was presumed on the basis of TLC results.</p>
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Characterization of the Murine Macrophage Suppressor Factor (Msf)
… can be partially purified by ultrafiltration and column chromatography. The molecular weight was determined to be between 2,344 and 1,318.
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