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Showing 1 to 20 of 23 for “"Chelex"”.
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Evaluation of Chelex 100 and Assessing the Impact of Fulvic Acid (NOM) on Copper Toxicity and Bioavailability to Americamysis bahia
A cation exchange method (Chelex 100) that distinguishes weakly bound and strongly bound copper was investigated for its ability to measure bioavailable copper in estuarine waters. Copper bound to the Chelex 100 resin was operationally defined as bioavailable copper. Varying initial copper …
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LITERATURE REVIEW: The Use of Cation-Exchange Resins in Natural Water Trace Metals Research. ARTICLE: Determination of Free Ion Trace Metal Concentrations in Freshwaters Using Cation-Exchange Resin Equilibrium. A Preliminary Investigation.
… Use of cation exchange resins, particularly Chelex-100, has been developed over the past few decades and enables preconcentration to yield, upon elution, measurable concentrations of trace metals. These resins can also yield data on the speciation (by measuring lability) of a metal in …
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The Speciation of Dissolved Uranium in Marine Waters
… different fractions from the same sample. Chelex-100 resin removes only inorganic or weakly bound trace metals from a solution, while the precipitates of iron hydroxide have been shown to remove variable amounts of dissolved organic matter as well.</p> <p>Although uranium is predicted to be …
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Trace Metal and Speciation Analysis using Ion-Exchange and Energy Dispersive X-ray Fluorescence Spectrometry
… (Dowex 50W-X8 and Dowex 1-X8) and chelation (Chelex-100) resins, in order to determine their physical and chemical characteristics, to understand and explain their limits of function and to optimise their use as substrates in trace metal and speciation measurement by EDXRF. Structural …
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Développement des sondes DGT spécifiques à l'échantillonnage in situ du radium à base des gels récepteurs de TK100, d'AG50W-X8, de CHELEX-100 et des oxydes de manganèse
...Si un enrichissement des teneurs en radium dans les échantillons environnementaux (sols, sédiments, eau, biote...) témoigne de la contamination d'un écosystème, les informations concernant sa mobilité, sa réactivité et sa biodisponibilité demeurent fragmentaires. C'est dans ce contexte que …
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Avaliação de métodos baseados na reação em cadeia da polimerase (PCR), para o diagnóstico de pacientes em tratamento da malária
… para serem processadas através da PCR: o Chelex e a Saponina. Com o Chelex, a PCR simples mostrou um limite de detecção de 700 parasitos/μl, enquanto que com a Saponina o limite de detecção foi de 70 parasitos/μl. A HNPCR e a STHNPCR apresentaram limites semelhantes de detecção sendo de 7 …
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The optimisation of protocols for the extraction of nuclear DNA from animal bone forPCR.
… profiles of different species. A modified Chelex method was selected as the preferred extraction procedure and RAPD-PCR profiles were created for several species using a number of different primers. Individuality between closely related species was also achieved. This research could go on …
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Use of molecular and bioinformatics tools for developing methods of epidemiological identification of trypanosomes
… Whatman FTA cards, an extraction method based on Chelex was investigated. Using Chelex to extractT. brucei DNA from FTA cards, followed by a nested ITS-PCR detection system, allowed parasite DNA detection to 1ng/µl. To evaluate this tool on field samples, ITS-PCR amplification was carried on DNA …
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Diffusive gradients in thin films (DGT) as a technique to predict nutrient availability to plants
… The MBL consisted of ferrihydrite and Chelex - 100 cation exchange resin combined together in a binding gel. Results from the MBL were comparable to experiments performed using individual Chelex gels and ferrihydrite gels that have been shown to work successfully for DGT methodology. To …
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THE DETERMINATION OF STABILITY CONSTANTS AND COMPLEXING CAPACITIES OF NATURAL ORGANIC MATTER FOR COPPER (2+), COBALT (2+) AND MANGANESE (2+) BY FLUORESCENCE QUENCHING TITRATION
… on porous glass beads are compared to Chelex 100. In the presence of humic materials, modeled with isolated soil-derived fulvic acid (SFA), the removal of metal ions with Chelex is significantly hindered while the stronger immobilized chelates still perform adequately.</p><p>A …
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In vivo metal substitution in bacteroides superoxide dismutase
… was decreased by treatment of the media with chelex-100, a metal-chelating resin, and addition of desferrioxamine mesylate (desferal, Ciba-Geigy), an iron chelator. Mn-containing (MnSOD) and Fe-containing superoxide dismutase (EeSOD) activities in cell extracts were differentiated by …
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Investigation of the Effects of Environmental Insults on Presumptive and Confirmatory Tests and DNA Degradation
… method could be used. The methods used were: Chelex-100, Phenol/chlorophorm, QIAamp® DNA Investigator Kit (Qiagen), InnuPREP® forensic extraction kit (Analytikjina), the EZ1® system using the investigator card (Qiagen), the AutoMate Express™ System using the PrepFiler™ Express kit (Applied …
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Population genetics and transmission dynamics of Plasmodium falciparum in the Kavango East and Zambezi regions of Namibia
… regions of Namibia. DNA was extracted using the chelex DNA extraction method, the parasite DNA was genotyped with capillary electrophoresis using a 26 microsatellite marker set to determine P. falciparum genetic structure in the Kavango East and Zambezi regions of Namibia The study through …
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Investigation into the genetic variation of Toll-Like Receptor 9 in cattle using both Sanger and next-generation sequencing from FTA-Cards
… FTA cards using three different approaches: 5% Chelex resin, the REPLI-g Mini (Qiagen) and the DNeasy Blood & Tissue Kit (Qiagen). The most efficient method for DNA extraction was via the DNeasy Blood & Tissue (Qiagen) kit and this allowed a PCR protocol to be developed for amplification of a …
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ΧΗΜΙΚΗ ΜΕΛΕΤΗ ΤΗΣ ΔΙΑΚΙΝΗΣΗΣ ΤΩΝ AL-FE-MN-CU-PB-ZN ΣΤΙΣ ΕΚΒΟΛΕΣ ΤΟΥ ΑΧΕΛΩΟΥ
… THE DISSOLVED METAL WAS PRECONCENTRATED WITH CHELEX-100 RESIN AND MEASURED WITH GFAAS. THE TRACE METAL CONTENT OF THE PARTICULATE MATTER AND THE SEDIMENTS WAS EXTRACTED EITHER BY USING COLD-DILUTED HCL OR BY USING HOT-CONC. HNO3, AND THEN MEASURED WITH AAS. IN ADDITION TO THE ABOVE, A SERIES …
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Trace element contaminants in the Kuwait water production system
… in the analysis. Solid-liquid extraction (chelex-100 resin) and liquid-liquid extraction (methyl iso-butyl ketone and freon) with and without back-extraction into nitric acid were investigated. Liquid-liquid extraction without back-extraction proved to give optimum recovery of the selected …
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Ecotoxicology and ecophysiology of mysids, with special reference to copper toxicity in Praunus flexuosus
… to provide a measurement of total and labile (Chelex-available) dissolved copper in the natural seawater used in the toxicity tests. Labile dissolved copper did not show significant variations in the test seawater, confirming that organisms were exposed to constant labile copper concentrations …
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Assessing the bioavailability of the radionuclides technetium-99, selenium-79 and uranium-238 in contaminated soils using the diffusive gradients in thin-films (DGT) technique
… and U in soils. Initially, the performance of a Chelex-ferrihydrite mixed binding layer (MBL) DGT for the novel simultaneous measurement of Se and U was investigated to validate its suitability for subsequent use. An assessment of the availability and aging of all three elements was centred on an …
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DESORPTION OF COPPER FROM ILLITE CLAY USING A CHELATING RESIN ENCAPSULATED BETWEEN POROUS MEMBRANES
… consists of a cassette containing 100 mg of Chelex-100 sandwiched between two 0.40 um Polycarbonate membranes. While the device is similar to the one described by Kerfoot and Vaccaro (1975), it has been modified from a passive system dependent upon diffusional processes to an active mode in …
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Measuring Bioavailable Copper in Estuarine Waters using an Ion Exchange Resin
Anion exchange methods were used to measure the percent of weakly bound and strongly bound copper in estuarine waters and agricultural drainage. Copper bound to an ion exchange resin has been operationally defined as bioavailable copper, or copper that is available for uptake by aquatic organisms. …
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