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Showing 1 to 20 of 199 for “"Calmodulin"”.
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Calmodulin and Calmodulin-Peptide Interactions: Structure, Energetics, and Dynamics
Calmodulin (CaM) is the primary transducer of calcium-mediated signal transduction in many eucaryotic cells. Understanding the interactions it makes with target proteins is central to understanding its role as a master regulator of cellular metabolism. Calmodulin is capable of binding dozens of …
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Biochemical characterization of calmodulin isoforms and identification of calmodulin-binding proteins in Arabidopsis
… concentration. The identification of four calmodulin (CaM) isoforms in Arabidopsis provides a good opportunity to address this fundamental question. The goal of this research was to address whether CaM isoforms confer specificity to Ca$\sp{2+}$-mediated signal transduction pathways. A …
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Studies on the Mechanism of Action of Calmodulin
… principles underlying the mechanism of action of calmodulin (CaM). This was approached through the study of the interactions of CaM with Ca('2+)+CaM sensitive cyclic nucleotide phosphodiesterase (PDE) and with troponin I. Because CaM has been found to behave in a similar manner in most, if not …
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Analysis of multiple calmodulin isoforms in Arabidopsis thaliana
… characterized calcium-binding protein is calmodulin (CaM), a 17 kDa protein which binds four calcium ions. Three calmodulin isoforms have previously been characterized in Arabidopsis thaliana, and in this study three additional cDNA sequences encoding calmodulin isoforms have been …
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DNA-protein interactions throughout the Arabidopsis calmodulin-3 gene
In Arabidopsis thaliana, calmodulin is encoded by a gene family of at least six members that generate four unique, but highly similar calmodulin polypeptides. In vitro techniques were used to identify putative cis-acting elements in the calmodulin-3 gene and the types of nuclear factors with which …
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Partial isolation and characterization of the mouse calmodulin genes
Three genes encode identical calmodulin proteins in mammals. One possible reason for this unique situation is that it allows differential regulation of calmodulin gene expression. In order to study the promoter regions of the different genes, the 5’ ends of three mouse calmodulin cDNAs were …
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Comparative Biochemistry of Drug-Binding Proteins in Calmodulin/S100 Family
<p>Calmodulin is a member of a class of structurally and functionally related proteins. Calmodulin is widely distributed among eukaryotes, and the amino acid sequence of calmodulin is highly conserved. The protein contains multiple structural domains and has multiple activities in vitro, including …
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Regulation of Transglutaminase by 5-HT2A Receptor Signaling and Calmodulin
… by which 5-HT2A receptor signaling and calmodulin (CaM) regulate TGase-catalyzed transamidation of Rac1 and htt in cultured neuronal cells and HD transgenic animals, respectively.</p><p>5-HT2A receptors are G-protein coupled receptors which are widely expressed in the brain, peripheral …
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PMCA as a regulator of calcium/calmodulin-dependent signal transduction pathways
Plasma membrane calcium/calmodulin-dependent calcium ATPases (PMCAs) are high affinity calcium pumps regulating many calcium-dependent processes and advances in its characterisation have discovered that it may play a novel role in signal transduction pathways. It was the aim of this work to further …
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Role of Neurogranin In The Regulation of Calcium Binding to Calmodulin
… in the regulation of calcium binding to Calmodulin</strong></p> <p><strong></strong>Anuja Chandrasekar, B.S</p> <p>Advisor: M. Neal Waxham, Ph.D</p> <p>The overall goal of my project was to gain a quantitative understanding of how the interaction between two proteins neurogranin (RC3) and …
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Isolation, characterization and expression of calmodulin genes from carrot and Arabidopsis
Calmodulin is a highly conserved calcium-binding protein universally distributed among eukaryotes. Although the role of calmodulin in regulatory pathways in animals is well characterized, information on similar pathways in plants is limited. To better understand the role of calmodulin in plant …
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The structure and sequence of the single calmodulin gene of Drosophila melanogaster
… and characterization of the gene encoding the calmodulin protein in the dipteran species, Drosophila melanogaster. The two major protein coding exons, designated 2 and 3, had been previously isolated. Together they encode 139 of a total of 148 amino acids. These exons were used to screen a D. …
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Characterisation of calcium/calmodulin-dependent protein kinase kinase 2 in prostate cancer
CaMKK2 is an enzyme which has been implicated in PCa tumorigenesis and progression by the regulation of glycolysis through activation of its substrate AMPK. However phenotypic effects observed in CaMKK2 KO mice and cell lines with CaMKK2 inhibition or silencing were not rescued by AMPK, suggesting …
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Partial Purification and Characterization of the Calmodulin Sensitive Bovine Brain Adenylate Cyclase
The calmodulin (CaM) sensitive bovine brain adenylate cyclase was resolved, in a solubilized form, from calmodulin and a CaM-insensitive form of the enzyme. Affi-Gel Blue chromatography, incorporating EGTA-containing buffer washes and elution with 8 mM ATP/l M KCl, produced partially purified …
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Structural Studies on Calcium/calmodulin-dependent Activation of Eukaryotic Elongation Factor 2 Kinase
… protein synthesis. Activated by calcium-loaded calmodulin (Ca<sup>2+</sup>-CaM), eEF-2K phosphorylates its only known physiological substrate, eEF-2, on a specific threonine residue (Thr-56). Phosphorylated eEF-2 has reduced affinity for the ribosome, and results in a significant decrease in the …
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Baculovirus-directed expression of the phosphorylase kinase catalytic subunit: pseudosubstrate and calmodulin regulation
Phosphorylase kinase (EC 2.7.1.38) is a key enzyme involved in the regulation of the glycogenolysis pathway. It catalyzes the Ca²⁺-dependent phosphorylation and activation of the enzyme glycogen phosphorylase to make the active form glycogen phosphorylase. Phosphorylase kinase is composed of 4 …
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