Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 76 for “"CD28"”.
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Modulation der Immunglobulinproduktion bei Ratten mit CD28-spezifischen monoklonalen Antikörpern
… co-stimulatorischen Rezeptoren auf T-Zellen ist CD28. In Abhängigkeit vom TZR-Signal nimmt CD28 Einfluss auf die Th1/Th2-Differenzierung der Immunantwort. Die rattenspezifischen mAk JJ316 und JJ319 binden an das CD28-Molekül und haben beide ein gleich starkes co-stimulatorisches Potential. Gabe …
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Engineering and Characterization of a Single Chain Antibody Specific for Murine CD28
Finally, a novel method of measuring the thermal stability of engineered antibody fragments using yeast surface display and flow cytometry was produced. This method allowed for the rapid determination of relative temperature stability without the need to produce large quantities of protein. This …
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Verstärkung und Modulation der Immunantwort der Ratte mit Hilfe eines superagonistischen, CD28-spezifischen, monoklonalen Antikörpers
… mit Spezifität für den kostimulierenden Rezeptor CD28 der Ratte untersucht. Dieser Antikörper unterscheidet sich von konventionellen CD28-spezifischen mAk durch seine Fähigkeit, T Zellen auch ohne Ligation des TZR und damit polyklonal zu aktivieren. Die so ausgelöste Expansion der T Zellen …
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Investigating the role of CD28 costimulation and IL-4/IL-13 responsive myeloid and lymphoid cells during helminth infections in mice
… of this study was to evaluate the importance of CD28 in initiating protective Th2 immunity against both primary and secondary infections with N. brasiliensis. Our findings demonstrate that CD28 is required for initiation of protective Th2 immunity against primary infection with N. brasiliensis. …
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Mechanismen der TZR-abhängigen und -unabhängigen Aktivierung primärer T-Zellen der Ratte durch CD 28
… den bekannten kostimulatorischen Molekülen ist CD28 das potenteste. Die mitogene Aktivität einiger monoklonaler Antikörper (mAb) spezifisch für CD28 der Ratte, die alle ruhenden primären Ratten-T-Zellen ohne TZR-Ligation zu Proliferation und IL-2-Produktion anregen können, erlaubte, die …
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Spatial Dynamics and the Mechanoresponse in CD4+ T Cell Activation
… TCR engagement and is further augmented by CD28 costimulation: ensuring T cell survival and conferring numerous functional capabilities. The work in this dissertation highlights the spatial and temporal dynamics that regulate the initial coupling of CD28 with TCR signaling and also dissects …
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Functional characterization of rat CTLA-4 and CD25+CD4+ regulatory T cells
… led to the deactivation of anti-TCR- and anti-CD28 stimulated (costimulation) T cell responses such as reduction in activation marker expression, proliferation, and cytokine IL-2 production. Although T cells stimulated with the superagonistic anti-CD28 antibody alone without TCR engagement also …
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The Characterization of T-Cell Manufacturing For Adoptive T-Cell Therapies
… In addition, I show that expression of CD28 correlates with multiple manufacturing metrics, including final T-cell fold expansion. I propose that the loss of CD28 expression creates a<em> </em>T-cell expansion bottleneck in which certain T-cell clones are heavily favored compared to …
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The Role of Chronically Stimulated and Senescent T Cells in Autoimmunity
… the course of the disease. Human T cells adopt a CD28-CD57+ phenotype in chronic viral infections and this has been hypothesized to result from chronic stimulation, however this phenotype may also be due to direct viral effects on T cells. Here I make use of human MS patients before and after …
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Herstellung und funktionelle Analyse von Immunzytokinen, die Interleukin-2 (IL-2) oder Granulozyten/Makrophagen Kolonie-stimulierenden Faktor (GM-CSF) enthalten, zur Verbesserung einer NDV-modifizierten Tumorvakzine
… hergestellten Konstrukte bs HN-CD3 und bs HN-CD28 sind so beschaffen, dass sie mit einem Arm an das virale Antigen Hämagglutinin-Neuraminidase (HN) auf der Oberfläche der Vakzinezellen binden. Der zweite Arm hingegen ist entweder gegen das T-Zell-Rezeptor-assoziierte CD3-Molekül oder gegen den …
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The role of immunosenescence in autoimmune/autoinflammatory pathogenesis of Behçet's disease
… of senescent CD4+ T cells (CD3+ CD4+ CD27- CD28- cells), CD8+ T cells (CD3+ CD8+ CD27- CD28- cells), and B cells (CD19+ CD27- IgD- cells) were analyzed. The differences among the groups, the correlation with chronological age in HC group, and whether the clinical indicators of disease …
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Staphylococcal Enterotoxin B Induced Clonal Anergy in αβ T Cell Receptor Transgenic Mice
… of costimulation, we examined the effect of the CD28/B7 pathway on the response of T cells to SEB and OVA. The T cell response to both OVA and SEB was enhanced by antibodies to the CD28 coreceptor suggesting that CD28/B7 mediated costimulation plays a role in the signal transduction pathways …
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Characterization and Optimization of Antigen-Specific T Cell Responses During Ex Vivo Expansion of Melanoma Tumor-Infiltrating Lymphocytes
… analysis revealed that the expression of CD28 was significantly reduced in post-REP TILs. By sorting experiment and microarray analysis, we confirmed that the few CD28+ post-REP TILs had superior survival capacity and proliferated after restimulation. We then went on to investigate methods …
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Applying single-molecule localisation microscopy to achieve virtual optical sectioning and study T-cell activation
… require the co-stimulatory triggering of the CD28 transmembrane receptor to become fully activated. However, some immobilised anti-CD28 antibodies, referred to as super-agonists (SA), can directly activate T cells without triggering the TCR. In this thesis, single-molecule tracking techniques …
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Characterising the gene regulatory landscape of CD4+ T cells
… receptor (TCR) recognition of an antigen and CD28 co-stimulation. The role of CD28 co-stimulation requirement in the activation of different T cell subsets has been understudied. Here, I assessed the role of immune disease variants in modulating pathways underlying T cell activation and Treg …
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Transcriptional Regulation of the Inducible Costimulator (ICOS) in T Cells
… inducible costimulator (ICOS) is a member of the CD28 family of costimulatory molecules and plays a central role in T-cell-mediated immunity. In this study, we investigated the molecular regulation of ICOS expression during early activation of CD4+ T cells by T cell receptor and CD28 co-engagement …
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The Role of Alternative Splicing and Alternative Polyadenylation in Regulating Activated T cell Biology
… of human naive CD4+ T cells stimulated with anti-CD28, anti-CD3, and anti-CD3/CD28 for 8 and 48hrs, to further elucidate the signal-specific regulation of alternative splicing and alternative polyadenylation changes upon stimulation. I show that splicing and polyadenylation changes that occur …
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