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Showing 1 to 20 of 107 for “"BrDU"”.
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Analysis of the effects of BrdU on DLKP human lung cancer cells by two-dimensional difference gel electrophoresis and mass spectrometry
Bromodeoxyuridine (BrdU) is a thymidiie analogue that incorporates into DNA of dividing cells during the S-phase of the cell cycle. Previous work in laboratories reported that treatment with lOyM BrdU in the human lung carcinoma cell line (DLKP) resulted in increased expression of the cytoskeletal …
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Determinants of cns adult neurogenesis are sex, hormones, mouse strain, age, and brain region
… callosum (CC), and parenchyma. With a short 2 h BrdU pulse, no gender and strain differences are present at 6-8wk. At 6-8mo, the number of BrdU+ cells decreases 2-fold in both sexes, strains and regions indicating a common aging mechanism regulates BrdU incorporation. Strikingly, 2X more BrdU+ …
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ATM-Dependent ERK Signaling in Response to DNA Double Strand Breaks
… carcinoma cells containing bromodeoxyuridine (BrdU)- substituted DNA were treated with Hoechst 33258 followed by long wave-length UV (UV-A) (BrdU photolysis). BrdU photolysis resulted in well-controlled, dose-dependent generation of DSBs equivalent to 0.2 - 20 Gy of IR, as detected by …
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Chronic mild social stress increases neurogenesis in adolescent male rats
… 1, rats were administered Bromodeoxyuridine (BrdU) daily on PND 30-32, or 46-48, to mark neurogenesis at the beginning of the stressor or after the stressor had ceased, respectively. Neither short-term nor chronic stress had an effect on proliferation or survival (evidenced by BrdU and …
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Διερεύνηση της επίδρασης της μικρονευροτροφίνης ΒΝΝ237 στην διαδικασία της νευρογένεσης υπό συνθήκες χρόνιου στρες
… το ΒΝΝ237, ενώ σε όλους τους μύες χορηγήθηκε BrdU (5-βρωμο-2’-δεοξυουριδίνη) είτε τρεις ημέρες (Cohort A), είτε 4 εβδομάδες (Cohort B) πριν την θανάτωσή τους. Τα αποτελέσματα της παρούσας μελέτης καταδεικνύουν ότι η μικρονευροτροφίνη ΒΝΝ237 αναστέλλει τη στρεσοεπαγόμενη μείωση του κυτταρικού …
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Gene amplification in Drosophila ovarian follicle cells as a developmental strategy and model for metazoan DNA replication
… and MCM2-7, as well as the nucleotide analog BrdU, at sites of gene amplification. These studies revealed that the BrdU staining pattern resolves from a focus of incorporation at the third chromosome locus in egg chamber stage lOB, to a coffee-bean structure in stage 11 egg chambers, to a …
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Vaskuläre Progenitorzellen und embryonale Angiogenese
… (14.-19. Entwicklungstag) 24 h lang mit BrdU inkubiert, das KM aus Tibia und Femur entnommen und auf die Chorioallantoismembran transplantiert. Nach mehrtägiger Reinkubation von Empfängern (7.-14. Entwicklungstag) wurden die CAM mit Transplantat sowie ausgewählte Organe des Empfängers …
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Proliferation, Migration, and Survival of Cells in the Telencephalon of the Ball Python, Python regius
… The thymidine analog 5-bromo-2’-deoxyuridine (BrdU) was used to quantify cell proliferation, migration, and survival in the ball python (<em>Python regius</em>). First, to determine the proper dose of BrdU for injection we subcutaneously injected 50mg/kg, 100mg/kg, and 250mg/kg into 15 adult …
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Long-term dispensation and molecular control of endogenous progenitor cells in adult primate telencephalon // Дългосрочна съдба и молекулен контрол на ендогенни прогениторни клетки в краен мозък на възрастни примати
… субекти бяха белязани с bromodeoxyuridine (BrdU), индикатор за ДНК синтеза. Впоследствие, ние изследвахме разпределение и фенотип на оцветени за BrdU клетки в няколко региона от крайния мозък, на различни интервали след исхемия. Исхемията доведе до значително увеличение на количествата de …
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Effects of Proton Radiation and Pomegranates on Hippocampus and Behavior
… was quantified using immunohistochemistry for BrdU+/DCX+ cells. Radiation suppressed cell proliferation (BrdU+) and neurogenesis in the dentate gyrus (BrdU+/DCX+) of the hippocampus. Pomegranate juice increased neurogenesis (DCX+) in the hippocampus of non-irradiated mice. These data highlight …
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Effects of Homocysteine on Cardiac Neural Crest Cell Formation
… on cell proliferation, 5-bromo-2'-deoxyuridine (BrdU) was used to label cells in S- phase of the cell cycle. In control embryos, a significantly higher percentage of newly formed NC cells were in S-phase compared to migrating NC cells or their progenitors within the neural tube. These results …
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Cell Kinetics of Osteoblast Histogenesis in Evolving Rabbit Secondary Haversian Systems Using a Double Labeling Technique with [³H]-Thymidine and Bromodeoxyuridine
… with [3 H]-thymidine and bromodeoxyuridine (BrdU), this study examined cell kinetics in 120 SHSs per rabbit at 12 hour intervals up to 72 hours after labeling. A total of 7 rabbits were injected with alizarin complexone (-1 O days: 20 mg/kg/day), tetracycline (-3 days: 10 mg/kg/day), …
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불멸화 유전자 도입을 통한 베타 세포주 구축
… Soft agar assay 16 6. Immunocytochemistry 16 7. BrdU incorporation assay 16 8. 단일 세포 선별 18 9. 인슐린 정량 18 10. RT-PCR 18 11. MTT assay 19 Ⅲ. 결과 23 Ⅳ. 고찰 49 Ⅴ. 결론 54 참고문헌 55 ABSTRACT 59"
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Effect of Epigallocatechin-3-gallate on a pattern separation task and hippocampal neurogenesis in a mouse model of Down syndrome
… for five days, received a 100mg/kg injection of BrdU, and were perfused two hours later. Coronal sections through the dorsal hippocampus were processed for BrdU labeling, and cells were manually counted throughout the subgranular zone of the dentate gyrus. The euploid controls had significantly …
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The Effect of Endothelin-1 on the expression of CDK Inhibitors p21 & p27 in Bovine Corneal Endothelial Cells
… with 10% serum. 5-Bromo 2-deoxy Uridine (BrdU) incorporation was determined in serum starved cultures in 24-well plates as a measure of cell proliferation. Confluent serum starved cells grown in T-25 flasks were treated with 100nM Endothelin-1 in DMEM. The control cells were left untreated …
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Effects of Food Consumption on Cell Proliferation in the Brain of Python regius
… the thymidine analog 5-bromo-12’-deoxyuridine (BrdU) to quantify and compare cell proliferation in the brain of fasted snakes and at two time points: two days and six days after a meal, which span time periods of during and after SDA response, respectively. Quantification of BrdU-labeled cells …
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Investigating the maintenance of the mouse definitive adrenal cortex
… cells are replenished in the adult mice. BrdU pulse-chase studies provided valuable information about cell division and cell fate under physiological or pathophysiological stimulations. The distribution of adrenocortical cells with nuclei stained positively for BrdU and/or Ki67 was …
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DOES CALCIUM INFLUX THROUGH T-TYPE CALCIUM CHANNEL INDUCE CARDIOMYOCYTE PROLIFERATION?
… in both α1G-/- NMVMs and WT NMVMs. Using BrdU, a DNA synthesis marker, we identified plenty of BrdU positive cardiomyocyte during the first seven days after birth. Cardiomyocyte is special due to its double nucleation property. Our cell cycle studies showed that there is significant …
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Modeling Neural Stem Cell Dynamics in Congenital Heart Disease
… origin of SVZ-derived neuroblasts. Following BrdU tracing, multiplex labeling, and confocal microscopy, I show that the porcine brain contains populations of newly generated (BrdU+/DCX+) neurons in the prefrontal cortex that are produced postnatally. Regional analyses using immunohistochemical …
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