Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 195 for “"B. subtilis"”.
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Mutations of the glucose-pts in sublancin-resistant B. subtilis 168 ΔSPΒ
… generating new sublancin-resistant mutants in B. subtilis 168 ΔSPβ. The mutations found support previous findings but also demonstrate the need to look for larger genome changes that may affect the regulation of the glucose phosphotransferase system.
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Diverse primary and secondary structural features are associated with Y complex-dependent mRNA maturation in B. subtilis
In Bacillus subtilis, mRNA processing and decay are primarily initiated by the endonuclease RNase Y. Recent work by our group discovered a set of auxiliary factors called the Y complex that is required for the mRNA maturation of 21 operons. It is unclear, however, how these operons specifically are …
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Understanding the role of TapA in the formation of <i>B. subtilis</i> biofilms: a biochemical and genetic analysis
… biofilm formed by the organism Bacillus subtilis. This study explores the properties of the extracellular matrix which gives structure to the biofilm and protects the encased cells from a variety of environmental threats.<br/><br/>The B. subtilis biofilm matrix is composed of …
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Molecular Cloning and Expression of the Pyrimidine B Gene From Bacillus Subtilis
The aspartate transcarbamylase of Bacillus subtilis is an example of a "vegetative" gene product which ceases to be synthesized once the cells begin to sporulate. To study the regulation of expression of this enzyme, the gene which encodes it (pyrB) was cloned from B. subtilis. Chromosomal DNA from …
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Investigation of Bacillus subtilis as a Biopesticide Against Botrytis cinerea
… Disease Control Spray (concentrated <em>Bacillus subtilis</em> strain QST 713). Both laboratory tests as well as <em>in vivo</em> lab tests were conducted. BiOWiSH<sup>TM</sup>-Aqua results varied widely from plate to plate, regardless of experimental conditions. In some of these plates, …
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Regulation and production of extracellular signaling molecules in Bacillus subtilis
… to alter gene expression. Bacillus subtilis uses cell-cell signals to control genetic competence, sporulation, degradative enzyme production and antibiotic synthesis. A family of peptide signals controls gene expression in B. subtilis by regulating transcription factors. I showed …
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Bacterial production of poly-γ-glutamic acid and evaluation of its effect on the viability of probiotic microorganisms
… the production of γ-PGA by eight bacteria (B. subtilis 23856, B. subtilis 23857, B. subtilis 23858 B. subtilis 23859, B. subtilis natto, B. licheniformis 1525, B. licheniformis 6816 and B. licheniformis 9945a) in GS and E media. B. subtilis natto and B. licheniformis 9945a have been …
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Genetic, biochemical and behavioral studies of the chemotactic response in Bacillus subtilis
… in the major adaptation pathway of Bacillus subtilis chemotaxis were cloned and sequenced. They were found to be homologous to the corresponding enzymes of the Escherichia coli system. While the B. subtilis proteins could substitute for the E. coli proteins, they play opposite roles in the …
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Non-canonical sensing mechanisms in bacteria
… aeruginosa, Helicobacter pylori, and Bacillus subtilis. In the past five decades the study of bacterial chemotaxis has been mainly focused on simple molecules, including amino acids and sugars, which are essential for cells growth and survival. Although the details of the underlying molecular …
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The Protein-protein Interaction Map of the Treponema pallidum Flagellar Apparatus
… Treponema-Interaktoren in E. coli und 30 in B. subtilis vorhergesagt. Die Gene von 23 Orthologen wurden dann in E. coli und/oder B. subtilis (7 Gene) mutiert und deren Phänotyp in Motilitätsassays überprüft. Ich fand dabei 10 neue Proteine in E. coli (amiA, bcsC, rfaH, rpe, rpmJ, ycfH, yciM, …
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Assessing the involvement of BslA in plant-root associated biofilms
… Gram-positive soil-dwelling bacterium Bacillus subtilis. B. subtilis biofilm formation is regulated by signalling networks that result in the production of two essential matrix components; an exopolysaccharide (EPS) and a fibrous protein (TasA), in addition to a bacterial hydrophobin (BslA). …
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Construction of plasmid-based expression and secretion vectors and study of the immobilization of proteins on the surface of Bacillus subtilis cells
… most of the available plasmids for Bacillus subtilis suffer from structural instability because of their rolling-circle replication mechanism. In this work, stable plasmids have been constructed allowing expression of recombinant proteins in the cytoplasm and their secretion into the culture …
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Investigating Epigenetics as a Mechanism to Regulate Bacterial Heterologous Expression
… hosts, namely gram-positive Bacillus subtilis, and gram-negative Escherichia coli and Pseudomonas putida. For the gram-negative strains, we confirmed the expected epigenetic methylation in the wildtype strains. Additionally for Pseudomonas putida, we generated four double-crossover …
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Biochemical and molecular characterization of chemotaxis genes in Bacillus subtilis
… gene, an essential gene for chemotaxis in B. subtilis was cloned, localized and characterized. This gene is contained in a 7.7 kb EcoR1 DNA fragment that was isolated from a $\lambda$ Charon 4A B. subtilis chromosomal DNA library. A 0.7 kb Pst 1 DNA fragment of this 7.7 kb EcoR1 fragment was …
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Molecular characterization of motility genes in Bacillus subtilis
… organisms such as Escherichia coli and Bacillus subtilis. Several genes involved in the process of flagellar synthesis have been identified and characterized from the che/fla operon of B. subtilis, a 26 kilobase (kb) transcription unit. One B. subtilis gene, designated as flhF, was found to …
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Negative Regulation of the accBC Operon in Escherichia Coli
Expression of the accB gene from B. subtilis complemented E. coli AccB activity, but did not cause negative regulation of the accBC genes. A chimeric protein consisting of the amino-terminal 44 amino acids of E. coli AccB fused to the carboxyl-terminal biotinylation domain of B. subtilis AccB …
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Chemotactic Sensory Transduction in Bacillus Subtilis: A New Enzyme and a New Pathway
… chemotaxis towards PTS carbohydrates by B. subtilis requires the transmembrane chemoreceptor McpC; therefore, B. subtilis must employ a different sensory circuit to mediate such chemotaxis. The results in this study indicate that the sensory circuit mediating chemotaxis towards PTS …
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Analyse der Funktion und Regulation des ECF-Sigmafaktors YlaC aus Bacillus subtilis
… In dem Gram-positiven Modellorganismus B. subtilis konnten bislang sieben Gene mit Ähnlichkeiten zu ECF-Sigmafaktoren identifiziert werden (sigX, sigW, sigM, sigY, sigV, sigZ und ylaC), wobei hauptsächlich sigX, sigW und sigM im Zentrum durchgeführter Untersuchungen standen. Im Rahmen der …
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Biochemistry and regulation of CheA in Bacillus subtilis chemotaxis
… described in this thesis shows that Bacillus subtilis CheA differs from other members of this family of bacterial histidine kinases in two respects: it is able to achieve its maximum phosphorylation potential at very low ATP concentrations, and the phosphorylated form of the enzyme is very …
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Characterizing the role of central carbon metabolism and cell wall stress responses in Bacillus subtilis cell wall synthesis
… the gram-positive rod-shaped bacterium Bacillus subtilis consists of a thick peptidoglycan (PG) layer and phosphate containing anionic polymers called teichoic acids. To maintain the rod shape, B. subtilis elongates its lateral cell wall first, followed by cell division. Both tasks are carried …
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