Adeno-associated virus rep78 expression in Arabidopsis thaliana

Adeno-associated virus type 2 (AAV-2) integrates preferentially into a defined site on human chromosome 19, and has been developed as a gene therapy vector. We propose to use this unique recombination event for site-specific integration of transgenes in plants. This strategy would alleviate problems associated with current plant transformation methods that integrate transgenes randomly. The AAV-2 gene encoding the enzyme that catalyzes the insertion (rep78) was introduced into Arabidopsis thaliana via Agrobacterium-mediated transformation. PCR and sequence analysis confirmed the presence of rep78 in two plant lines. RT-PCR demonstrated rep78 transcription in one plant line, but protein could not be detected in either line.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Biology

Department dc:contributor.department

Biology

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

2002

Author dc:creator

• Sisco, Daniel

Chair dc:contributor.committeechair

• Lederman, Muriel L.

Committee members dc:contributor.committeemember

• Winkel, Brenda S. J.
• Rutherford, Charles L.

• plant transformation
• Adeno-associated virus

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Dc Identifier Other

etd-09222002-193552

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/9706