{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/9706"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/9706","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Adeno-associated virus rep78 expression in Arabidopsis thaliana","abstract":"Adeno-associated virus type 2 (AAV-2) integrates preferentially into a defined site on human chromosome 19, and has been developed as a gene therapy vector. We propose to use this unique recombination event for site-specific integration of transgenes in plants. This strategy would alleviate problems associated with current plant transformation methods that integrate transgenes randomly. The AAV-2 gene encoding the enzyme that catalyzes the insertion (rep78) was introduced into Arabidopsis thaliana via Agrobacterium-mediated transformation. PCR and sequence analysis confirmed the presence of rep78 in two plant lines. RT-PCR demonstrated rep78 transcription in one plant line, but protein could not be detected in either line.","abstract_html":"Adeno-associated virus type 2 (AAV-2) integrates preferentially into a defined site on human chromosome 19, and has been developed as a gene therapy vector. We propose to use this unique recombination event for site-specific integration of transgenes in plants. This strategy would alleviate problems associated with current plant transformation methods that integrate transgenes randomly. The AAV-2 gene encoding the enzyme that catalyzes the insertion (rep78) was introduced into Arabidopsis thaliana via Agrobacterium-mediated transformation. PCR and sequence analysis confirmed the presence of rep78 in two plant lines. RT-PCR demonstrated rep78 transcription in one plant line, but protein could not be detected in either line.","abstract_has_math":false,"creators":["Sisco, Daniel"],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Biology","degree_department":"Biology","school":null,"contributors":[],"advisors":[],"committee_chairs":["Lederman, Muriel L."],"committee_members":["Winkel, Brenda S. J.","Rutherford, Charles L."],"year":2002,"date_issued":"2002-09-06","date_published":"2002-09-06","updated_at":"2026-07-22T22:20:40Z","subjects":["plant transformation","Adeno-associated virus"],"languages":[],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-09222002-193552"],"render_values":[{"text":"etd-09222002-193552","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/9706","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Lederman, Muriel L."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Winkel, Brenda S. 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We propose to use this unique recombination event for site-specific integration of transgenes in plants. This strategy would alleviate problems associated with current plant transformation methods that integrate transgenes randomly. The AAV-2 gene encoding the enzyme that catalyzes the insertion (rep78) was introduced into Arabidopsis thaliana via Agrobacterium-mediated transformation. PCR and sequence analysis confirmed the presence of rep78 in two plant lines. RT-PCR demonstrated rep78 transcription in one plant line, but protein could not be detected in either line."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["ETD"]},{"key":"dc:title","label":"Title","values":["Adeno-associated virus rep78 expression in Arabidopsis thaliana"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Lederman, Muriel L."],"dc:contributor.committeemember":["Winkel, Brenda S. 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