An ultrastructural and immunocytochemical study of the wheat soil-borne mosaic virus, Polymyxa graminis relationship

The effects of various fixatives on viral suspensions, the progress of wheat soil-borne mosaic virus (WSBMV) through Florida 302 wheat, and WSBMV relationship to Polymyxa graminis Led. were studied at the subcellular level. The utilization of electron immunocytochemical techniques using gold-labelled antibodies on viral suspensions and thin sections confirmed that (1) paraformaldehyde provided the best preservation of viral morphology, viral concentration, and antigenicity; (2) P; graminis is the actual vector of the virus; and (3) the labelling pattern of the fungal structures and plant chloroplasts suggests that they may play a role in the synthesis of some viral proteins.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Plant Pathology

Department dc:contributor.department

Plant Pathology

Grantor dc:publisher

Virginia Polytechnic Institute and State University

Year dc:date.issued

1989

Author dc:creator

• Mayes, Mary Sue

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Language dc:language.iso

en

Handle dc:identifier.uri

http://hdl.handle.net/10919/53230

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/53230