{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/53230"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/53230","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"An ultrastructural and immunocytochemical study of the wheat soil-borne mosaic virus, Polymyxa graminis relationship","abstract":"The effects of various fixatives on viral suspensions, the progress of wheat soil-borne mosaic virus (WSBMV) through Florida 302 wheat, and WSBMV relationship to Polymyxa graminis Led. were studied at the subcellular level. The utilization of electron immunocytochemical techniques using gold-labelled antibodies on viral suspensions and thin sections confirmed that (1) paraformaldehyde provided the best preservation of viral morphology, viral concentration, and antigenicity; (2) P; graminis is the actual vector of the virus; and (3) the labelling pattern of the fungal structures and plant chloroplasts suggests that they may play a role in the synthesis of some viral proteins.","abstract_html":"The effects of various fixatives on viral suspensions, the progress of wheat soil-borne mosaic virus (WSBMV) through Florida 302 wheat, and WSBMV relationship to Polymyxa graminis Led. were studied at the subcellular level. The utilization of electron immunocytochemical techniques using gold-labelled antibodies on viral suspensions and thin sections confirmed that (1) paraformaldehyde provided the best preservation of viral morphology, viral concentration, and antigenicity; (2) P; graminis is the actual vector of the virus; and (3) the labelling pattern of the fungal structures and plant chloroplasts suggests that they may play a role in the synthesis of some viral proteins.","abstract_has_math":false,"creators":["Mayes, Mary Sue"],"institution":"Virginia Polytechnic Institute and State University","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Plant Pathology","degree_department":"Plant Pathology","school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1989,"date_issued":"1989","date_published":"1989","updated_at":"2026-07-22T22:20:00Z","subjects":[],"languages":["en"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10919/53230","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.department","label":"Department","values":["Plant Pathology"]},{"key":"dc:creator","label":"Author","values":["Mayes, Mary Sue"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2015-06-23T19:09:52Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2015-06-23T19:09:52Z"]},{"key":"dc:date.issued","label":"Date","values":["1989"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Polytechnic Institute and State University"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Plant Pathology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/53230"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The effects of various fixatives on viral suspensions, the progress of wheat soil-borne mosaic virus (WSBMV) through Florida 302 wheat, and WSBMV relationship to Polymyxa graminis Led. were studied at the subcellular level. The utilization of electron immunocytochemical techniques using gold-labelled antibodies on viral suspensions and thin sections confirmed that (1) paraformaldehyde provided the best preservation of viral morphology, viral concentration, and antigenicity; (2) P; graminis is the actual vector of the virus; and (3) the labelling pattern of the fungal structures and plant chloroplasts suggests that they may play a role in the synthesis of some viral proteins."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["An ultrastructural and immunocytochemical study of the wheat soil-borne mosaic virus, Polymyxa graminis relationship"]}]}],"canonical_facts":{"dc:contributor.department":["Plant Pathology"],"dc:creator":["Mayes, Mary Sue"],"dc:date.accessioned":["2015-06-23T19:09:52Z"],"dc:date.available":["2015-06-23T19:09:52Z"],"dc:date.issued":["1989"],"dc:description.abstract":["The effects of various fixatives on viral suspensions, the progress of wheat soil-borne mosaic virus (WSBMV) through Florida 302 wheat, and WSBMV relationship to Polymyxa graminis Led. were studied at the subcellular level. 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