Functional Consequences of Matrix Metalloproteinase-1 Over-Expression in Human Gliomas

Malignant brain tumors are among the deadliest of human cancers. Despit recent advancements in conventional therapies, glioblastomas remain incurable, largel y due to their ability to invade surrounding tissue. Matrix metalloproteinases are thought to contribute to the invaseive phenotype of human gliomas. Absent in normal brain, matrix metalloproteinase-1 (MMP-1) has been shown to be present in gliomas, and in particular in glioblastoma multiforme (GBM). To begin to examine the role of MMP-1 in these tumore, two human glioma cell lines were stably transfected with MMP-1 cDNA. Confirmation of MMP-1 over-expression in these cells was achieved through real-time PCR and Western blot analysis. The functional consequences of MMP-1 over-expression were analyzed using a collagen type-I invasion assay along with clonogenic and ATP viability assays. Data presented demonstrate that MMP-1 over-expressing cells were more invasive in both cell types and interestingly more clonogenic in on of the glioma cell lines, supporting a possible role for MMP-1 in glioma growth and invasion.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Physiology

Year dc:date.available

2006

Author dc:creator

• Mullet, Emily

Contributors dc:contributor

• Dr. Helen L. Fillmore

• MMP-1
• GBM
• matrix metalloproteinases
• brain tumor
• Life Sciences
• Physiology

Statement dc:rights

• © The Author

Identifier

https://scholarscompass.vcu.edu/etd/778

OAI identifier oai:identifier

oai:scholarscompass.vcu.edu:etd-1777